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Gene
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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_09055 and AO353_09060 are separated by 178 nucleotides
AO353_09060 and AO353_09065 are separated by 102 nucleotides
AO353_09065 and AO353_09070 are separated by 213 nucleotides
AO353_09070 and AO353_09075 are separated by 33 nucleotides
AO353_09055: AO353_09055 - HAD family hydrolase, at 1,987,882 to 1,988,586
_09055
AO353_09060: AO353_09060 - hypothetical protein, at 1,988,765 to 1,989,091
_09060
AO353_09065: AO353_09065 - hypothetical protein, at 1,989,194 to 1,989,619
_09065
AO353_09070: AO353_09070 - ammonia channel protein, at 1,989,833 to 1,991,170
_09070
AO353_09075: AO353_09075 - transcriptional regulator, at 1,991,204 to 1,991,542
_09075
Group
Condition
AO353
_09055
AO353
_09060
AO353
_09065
AO353
_09070
AO353
_09075
stress
Cobalt chloride 0.32 mM
-0.8
-2.9
-1.0
-0.1
N.D.
nitrogen source
Uridine (N)
-0.4
-2.7
-0.3
-1.3
N.D.
stress
Sisomicin 0.008 mg/ml
-0.2
-3.1
-0.6
-0.2
N.D.
stress
Paraquat dichloride 0.032 mg/mL
-0.0
-2.9
-1.1
+0.1
N.D.
stress
Cobalt chloride 0.32 mM
-0.3
-1.9
-0.8
-0.2
N.D.
stress
Tetracycline 0.0008 mg/ml
+0.1
-2.6
-0.7
+0.0
N.D.
carbon source
m-Inositol (C)
-0.2
-2.2
-0.5
-0.1
N.D.
carbon source
m-Inositol (C)
-0.1
-1.7
-1.0
-0.2
N.D.
carbon source
acetate (C)
-0.2
-1.6
-1.1
-0.0
N.D.
nitrogen source
Thymine (N)
-0.2
-1.1
-0.3
-1.2
N.D.
carbon source
L-Phenylalanine (C)
-0.6
-1.4
-0.7
-0.1
N.D.
carbon source
L-Leucine (C)
-0.3
-1.9
-0.4
-0.2
N.D.
carbon source
D-Mannose (C)
-0.1
-1.9
-0.6
-0.0
N.D.
stress
Spectinomycin 0.15 mg/ml
+0.1
-1.7
-0.8
-0.1
N.D.
nitrogen source
Glycine (N)
-0.3
+0.1
-0.2
-1.9
N.D.
anaerobic
N1B4wt with N2E3m denitrifying replicate C
+0.1
-2.4
-0.2
+0.1
N.D.
stress
Benzalkonium Chloride 0.012 mg/ml
-0.3
-2.3
+0.3
-0.1
N.D.
stress
Benzethonium chloride 0.025 mM
+0.6
-2.2
-0.1
-0.2
N.D.
carbon source
acetate (C)
+0.1
-0.8
-1.3
+0.3
N.D.
stress
Rifampicin 0.005 mg/ml
+0.6
-1.4
-0.5
+0.1
N.D.
nitrogen source
L-Threonine (N)
+0.2
+0.7
+0.0
-2.1
N.D.
carbon source
L-Ornithine
+0.3
+1.5
-1.6
-0.1
N.D.
carbon source
butyrate (C)
-0.3
+2.3
+0.0
-0.2
N.D.
carbon source
L-Ornithine
+0.7
+2.1
-0.5
-0.4
N.D.
carbon source
L-Aspartic Acid (C)
+0.3
+2.0
+0.4
+0.2
N.D.
carbon source
L-Asparagine (C)
+0.2
+2.0
+1.0
+0.1
N.D.
motility
outer cut, LB soft agar motility assay
+0.7
+1.3
+1.0
+0.5
N.D.
carbon source
L-Asparagine (C)
+0.3
+2.4
+1.2
+0.1
N.D.
carbon source
L-Ornithine
-0.3
+4.1
+0.8
+0.1
N.D.
carbon source
L-Serine (C)
+0.3
+4.6
+0.9
-0.1
N.D.
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