Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E3

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500 ntAO353_08850 and AO353_08855 are separated by 120 nucleotidesAO353_08855 and AO353_08860 are separated by 254 nucleotidesAO353_08860 and AO353_08865 are separated by 34 nucleotidesAO353_08865 and AO353_08870 overlap by 4 nucleotides AO353_08850: AO353_08850 - thioredoxin, at 1,945,677 to 1,946,006 _08850 AO353_08855: AO353_08855 - GntR family transcriptional regulator, at 1,946,127 to 1,946,831 _08855 AO353_08860: AO353_08860 - amino acid ABC transporter substrate-binding protein, at 1,947,086 to 1,947,871 _08860 AO353_08865: AO353_08865 - ABC transporter permease, at 1,947,906 to 1,948,574 _08865 AO353_08870: AO353_08870 - amino acid ABC transporter permease, at 1,948,571 to 1,949,221 _08870
Group Condition AO353_08850 AO353_08855 AO353_08860 AO353_08865 AO353_08870
carbon source L-Citrulline N.D. -0.9 -0.7 +0.3 -0.2
stress Doxycycline hyclate 0.0006 mg/ml N.D. -0.3 -0.3 -1.1 +0.3
stress Tetracycline 0.0008 mg/ml N.D. -0.7 +0.1 -0.3 -0.2
carbon source Cytidine (C) N.D. +0.1 -0.6 -0.1 -0.4
nitrogen source D-Alanine (N) N.D. -0.3 +0.1 -0.2 -0.4
carbon source L-Leucine (C) N.D. -0.7 -0.4 +0.4 -0.1
stress Sisomicin 0.008 mg/ml N.D. -0.9 +0.0 +0.0 +0.3
nitrogen source Gly-Glu (N) N.D. -0.4 -0.2 +0.4 -0.3
motility outer cut, LB soft agar motility assay N.D. -0.4 -0.4 +0.4 +0.1
anaerobic Anaerobic, acetate donor; nitrate and iron N.D. -0.7 +0.1 +0.1 +0.2
anaerobic N1B4wt with N2E3m denitrifying replicate C N.D. -0.9 +0.4 +0.1 +0.2
carbon source L-Malic (C) N.D. +0.5 -0.3 +0.3 -0.3
carbon source L-Citrulline N.D. -0.3 +0.7 -0.0 -0.3
stress Lomefloxacin 0.001 mM N.D. -0.4 -0.3 +0.5 +0.4
stress copper (II) chloride 2.5 mM N.D. +0.3 +0.4 -0.6 -0.0
stress Cisplatin 0.006 mg/ml N.D. -0.4 +0.1 +0.7 -0.1
motility outer cut, LB soft agar motility assay N.D. -0.4 +0.7 +0.4 -0.2
stress 1-ethyl-3-methylimidazolium acetate 15 mM N.D. +0.3 +0.5 -0.3 +0.2
carbon source D-Glucose N.D. +0.4 +0.2 +0.3 -0.2
lb LB N.D. +0.5 +0.4 -0.2 +0.1
carbon source L-Citrulline N.D. +0.2 +0.4 +0.6 -0.4
carbon source acetate (C) N.D. -0.4 +0.4 +0.8 +0.0
carbon source L-Ornithine N.D. +0.5 +0.3 +0.2 -0.2
carbon source L-Ornithine N.D. +0.5 -0.0 +0.5 -0.0
carbon source L-Serine (C) N.D. +0.4 -0.2 +0.8 +0.0
stress Gentamicin 0.006 mg/ml N.D. +0.7 +0.4 -0.0 +0.0
stress Chloride 500 mM N.D. +0.6 +0.2 +0.2 +0.2
carbon source acetate (C) N.D. +0.6 +0.4 +0.6 -0.2
stress 1-ethyl-3-methylimidazolium lysine 10.5 mM N.D. +0.4 +0.6 +0.2 +0.2
survival 4C survival; 672 hrs N.D. +0.1 +0.4 +1.0 +0.5
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