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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_08575 and AO353_08580 are separated by 187 nucleotides
AO353_08580 and AO353_08585 are separated by 199 nucleotides
AO353_08585 and AO353_08590 are separated by 53 nucleotides
AO353_08590 and AO353_08595 are separated by 667 nucleotides
AO353_08575: AO353_08575 - ABC transporter substrate-binding protein, at 1,883,539 to 1,884,633
_08575
AO353_08580: AO353_08580 - spermidine/putrescine ABC transporter substrate-binding protein, at 1,884,821 to 1,885,915
_08580
AO353_08585: AO353_08585 - Gamma-aminobutyrate:alpha-ketoglutarate aminotransferase (EC 2.6.1.19)
(from data)
, at 1,886,115 to 1,887,479
_08585
AO353_08590: AO353_08590 - glutamine synthetase, at 1,887,533 to 1,888,891
_08590
AO353_08595: AO353_08595 - Gamma-glutamyl-putrescine synthetase (EC 6.3.1.11)
(from data)
, at 1,889,559 to 1,890,935
_08595
Group
Condition
AO353
_08575
AO353
_08580
AO353
_08585
AO353
_08590
AO353
_08595
carbon source
Putrescine (C)
+0.4
-4.0
-2.0
-2.5
-1.2
nitrogen source
Putrescine (N)
+0.1
-1.2
-0.5
-0.0
-1.9
carbon source
acetate (C)
+1.1
-0.7
+0.5
-1.7
-2.3
carbon source
L-Arginine (C)
+0.3
-0.3
-0.1
+0.1
-3.1
carbon source
acetate (C)
+2.8
-1.5
+0.3
-2.3
-2.1
carbon source
L-Arginine (C)
-0.1
-0.1
+0.0
+0.2
-2.8
motility
outer cut, LB soft agar motility assay
-0.5
-0.1
+0.3
-0.5
-0.3
nitrogen source
L-Lysine (N)
+1.4
-2.0
-0.3
-0.2
+0.1
carbon source
propionate (C)
-0.6
-0.5
+0.2
+0.3
-0.3
nitrogen source
L-Lysine (N)
+1.3
-1.7
-0.4
-0.4
+0.3
nitrogen source
Adenosine (N)
-0.7
-0.6
+0.2
+0.2
+0.2
stress
methylglyoxal 0.009 vol%
+0.2
-0.2
-0.4
-0.7
+0.4
anaerobic
Anaerobic, acetate donor; nitrate and iron
+0.2
+0.2
+0.2
-0.8
-0.3
stress
Tetracycline 0.0016 mg/ml
-0.7
+0.0
-0.3
+0.6
-0.2
carbon source
L-Citrulline (C)
+0.6
+0.0
+0.2
+0.5
-1.8
stress
Rifampicin 0.005 mg/ml
-0.6
+0.1
+0.1
+0.5
+0.2
stress
Doxycycline hyclate 0.0002 mg/ml
-0.5
-0.0
+0.2
+0.6
+0.1
stress
Fusidic 0.125 mg/ml
-0.4
+0.3
+0.2
+0.5
-0.1
stress
Doxycycline hyclate 0.0006 mg/ml
-0.5
+0.2
+0.3
+1.0
+0.0
carbon source
L-Citrulline
+0.3
-0.3
+0.3
+0.6
+0.0
stress
acetate 34 mM
+0.3
+0.2
-0.1
-0.1
+0.7
stress
Cholin acetate 38 mM
+0.5
+0.2
+0.1
-0.2
+0.5
stress
Cisplatin 0.006 mg/ml
+0.7
-0.3
+0.6
+0.5
-0.1
carbon source
L-Ornithine
-0.0
+0.1
-0.1
+0.2
+1.7
stress
Cisplatin 0.006 mg/ml
+1.0
-0.0
+0.4
+0.7
+0.6
pH
Minimal media pH9
+0.8
-0.2
+0.8
+1.0
+0.3
carbon source
L-Ornithine
+0.1
+0.2
+0.2
+0.1
+2.3
pH
Minimal media pH9
+1.3
-0.4
+0.9
+1.1
+0.4
carbon source
L-Ornithine
-0.4
+0.5
+0.2
-0.3
+3.5
carbon source
L-Ornithine
-0.3
+0.2
+0.1
-0.1
+4.6
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