Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E3

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500 ntAO353_08525 and AO353_08530 are separated by 2 nucleotidesAO353_08530 and AO353_08535 are separated by 132 nucleotidesAO353_08535 and AO353_08540 are separated by 127 nucleotidesAO353_08540 and AO353_08545 are separated by 109 nucleotides AO353_08525: AO353_08525 - purine nucleoside phosphorylase, at 1,873,540 to 1,874,025 _08525 AO353_08530: AO353_08530 - 2OG-Fe(II) oxygenase, at 1,874,028 to 1,874,660 _08530 AO353_08535: AO353_08535 - hypothetical protein, at 1,874,793 to 1,875,545 _08535 AO353_08540: AO353_08540 - alpha/beta hydrolase, at 1,875,673 to 1,876,626 _08540 AO353_08545: AO353_08545 - thiol-disulfide isomerase, at 1,876,736 to 1,877,320 _08545
Group Condition AO353_08525 AO353_08530 AO353_08535 AO353_08540 AO353_08545
survival 4C survival; 672 hrs +0.3 -0.1 -0.2 -1.7 -0.7
survival 4C survival; 360 hrs -0.5 +0.2 -0.4 -0.6 -0.7
pH Minimal media pH9 -0.4 -0.4 -1.0 -0.2 -0.0
carbon source propionate (C) -0.6 -0.3 -0.6 -0.2 -0.3
motility outer cut, LB soft agar motility assay -0.5 +0.2 -0.7 +0.1 -0.9
anaerobic Anaerobic, acetate donor; nitrate and iron -1.6 +0.3 -0.3 +0.1 +0.4
pH Minimal media pH9 -0.6 -0.4 -0.3 -0.1 +0.4
carbon source L-Ornithine -0.2 -0.0 +0.1 +0.5 -1.2
survival 4C survival; 192 hrs +0.4 +0.2 -0.4 -0.1 -0.7
stress 1-ethyl-3-methylimidazolium acetate 15 mM -0.2 +0.3 -0.8 +0.3 -0.2
motility outer cut, LB soft agar motility assay +0.5 +0.4 -1.0 +0.3 -0.9
carbon source L-Citrulline -1.0 +0.3 +0.4 +0.4 -0.6
carbon source L-Ornithine -0.4 -0.1 +0.2 +0.6 -0.4
stress Sisomicin 0.008 mg/ml -0.0 -0.5 +1.0 -0.3 -0.4
stress acetate 68 mM +0.5 +0.1 -0.9 +0.2 +0.2
carbon source L-Citrulline +0.3 +0.2 -0.1 +0.7 -1.0
stress Cholin acetate 38 mM +0.5 +0.2 -0.8 +0.4 +0.0
carbon source L-Citrulline -0.3 +0.3 -0.3 +0.8 -0.2
carbon source D-Glucose +0.4 +0.1 +0.3 +0.5 -0.8
motility outer cut, LB soft agar motility assay +1.2 +0.6 -0.5 -0.4 -0.4
stress Cholin acetate 19 mM +0.2 +0.2 -0.6 +0.5 +0.3
carbon source L-Ornithine +0.7 +0.2 -0.0 +0.6 -0.5
carbon source L-Ornithine +0.1 +0.3 +0.2 +1.0 -0.3
carbon source Ethanol (C) -0.2 +0.4 +0.0 +0.4 +0.8
carbon source L-Citrulline +0.2 -0.2 +0.2 +0.7 +0.6
stress methylglyoxal 0.009 vol% -0.1 +0.1 +1.0 +0.0 +0.6
carbon source L-Alanine (C) +0.5 -0.1 +0.5 +0.2 +0.4
stress Fusidic 0.125 mg/ml +0.5 -0.4 +1.4 -0.4 +0.6
stress Cisplatin 0.006 mg/ml -0.3 -0.2 +0.9 +0.7 +0.8
stress Cisplatin 0.006 mg/ml -0.5 +0.3 +1.2 +0.6 +1.0
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