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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_08105 and AO353_08110 are separated by 234 nucleotides
AO353_08110 and AO353_08115 are separated by 77 nucleotides
AO353_08115 and AO353_08120 are separated by 240 nucleotides
AO353_08120 and AO353_08125 overlap by 1 nucleotides
AO353_08105: AO353_08105 - pilus assembly protein PilG, at 1,790,186 to 1,790,590
_08105
AO353_08110: AO353_08110 - glutathione synthetase, at 1,790,825 to 1,791,778
_08110
AO353_08115: AO353_08115 - energy transducer TonB, at 1,791,856 to 1,792,755
_08115
AO353_08120: AO353_08120 - hypothetical protein, at 1,792,996 to 1,793,565
_08120
AO353_08125: AO353_08125 - Holliday junction resolvase, at 1,793,565 to 1,794,002
_08125
Group
Condition
AO353
_08105
AO353
_08110
AO353
_08115
AO353
_08120
AO353
_08125
stress
methylglyoxal 0.008 vol%
-0.5
-3.5
-1.6
-0.3
N.D.
carbon source
propionate (C)
-0.2
-2.9
-2.4
+0.7
N.D.
stress
Cholin acetate 19 mM
-0.2
-1.9
-1.7
-0.6
N.D.
pH
Minimal media pH9
-0.6
-1.6
-1.6
-0.5
N.D.
stress
1-ethyl-3-methylimidazolium acetate 30 mM
-1.2
-1.2
-2.2
+0.3
N.D.
stress
Cholin acetate 38 mM
-0.5
-2.1
-1.5
-0.1
N.D.
survival
4C survival; 672 hrs
-1.5
-0.3
-2.1
-0.2
N.D.
stress
acetate 34 mM
+0.2
-2.2
-1.7
+0.1
N.D.
nitrogen source
D-Glucosamine Hydrochloride (N)
-0.4
+0.2
-2.9
-0.4
N.D.
stress
acetate 17 mM
-0.2
-1.8
-1.3
-0.2
N.D.
stress
Cobalt chloride 0.32 mM
-0.4
-2.7
-1.0
+0.6
N.D.
survival
4C survival; 192 hrs
-0.3
-0.3
-2.7
-0.2
N.D.
stress
1-ethyl-3-methylimidazolium acetate 15 mM
+0.0
-2.0
-1.4
-0.0
N.D.
stress
acetate 68 mM
-0.1
-2.1
-1.5
+0.5
N.D.
stress
Spectinomycin 0.2 mg/ml
-1.2
-1.8
-0.2
+0.0
N.D.
stress
methylglyoxal 0.009 vol%
+0.7
-2.5
-1.1
-0.4
N.D.
carbon source
propionate (C)
+0.1
-2.5
-1.6
+0.8
N.D.
stress
1-ethyl-3-methylimidazolium acetate 20 mM
+0.3
-1.7
-1.5
-0.0
N.D.
stress
Furfuryl Alcohol 0.25 vol%
+0.6
-2.1
-1.1
-0.0
N.D.
motility
outer cut, LB soft agar motility assay
-0.3
-0.7
-2.1
+0.4
N.D.
motility
outer cut, LB soft agar motility assay
+1.0
-0.7
-2.1
-0.6
N.D.
carbon source
Uridine (C)
+0.2
+0.7
-2.4
-0.4
N.D.
carbon source
Tween 20 (C)
-0.4
-0.7
-1.2
+0.9
N.D.
stress
Rifampicin 0.005 mg/ml
-0.6
+0.9
-1.5
-0.2
N.D.
stress
Doxycycline hyclate 0.0006 mg/ml
+0.1
+0.9
-0.5
-1.9
N.D.
stress
Lomefloxacin 0.001 mM
-1.1
+2.2
-1.7
-0.6
N.D.
nitrogen source
L-Phenylalanine (N)
+0.4
+1.0
-1.5
+0.3
N.D.
stress
nitrate 200 mM
+0.2
+2.3
-2.0
+0.1
N.D.
stress
Fusidic 0.125 mg/ml
-0.1
+2.3
-0.6
-0.6
N.D.
stress
perchlorate 75 mM
+0.3
+3.2
-1.8
+0.1
N.D.
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