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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_07770 and AO353_07775 are separated by 446 nucleotides
AO353_07775 and AO353_07780 are separated by 431 nucleotides
AO353_07780 and AO353_07785 are separated by 66 nucleotides
AO353_07785 and AO353_07790 overlap by 4 nucleotides
AO353_07770: AO353_07770 - AraC family transcriptional regulator, at 1,710,766 to 1,711,869
_07770
AO353_07775: AO353_07775 - serine dehydratase, at 1,712,316 to 1,713,692
_07775
AO353_07780: AO353_07780 - Carnitine ABC transporter, substrate-binding component CaiX
(from data)
, at 1,714,124 to 1,715,074
_07780
AO353_07785: AO353_07785 - ABC transporter for Carnitine, permease component
(from data)
, at 1,715,141 to 1,715,986
_07785
AO353_07790: AO353_07790 - ABC transporter for Carnitine, ATPase component
(from data)
, at 1,715,983 to 1,717,161
_07790
Group
Condition
AO353
_07770
AO353
_07775
AO353
_07780
AO353
_07785
AO353
_07790
carbon source
Carnitine Hydrochloride (C)
-3.2
-0.6
-3.0
-3.8
-2.9
nitrogen source
Carnitine Hydrochloride (N)
-0.9
-0.4
-1.0
-1.5
-2.1
carbon source
L-Leucine (C)
-1.0
-0.4
+0.1
-0.2
-0.2
survival
4C survival; 672 hrs
-0.0
+0.7
-1.5
-0.0
-0.1
stress
Cholin acetate 38 mM
-0.3
+0.4
-0.2
-0.2
-0.6
carbon source
L-Ornithine
-0.1
-0.0
+0.3
-1.6
+0.6
carbon source
L-Phenylalanine (C)
+0.3
+0.1
+0.2
-0.8
-0.5
stress
nitrate 200 mM
+0.2
-0.0
+0.2
-0.4
-0.6
carbon source
L-Citrulline
-0.7
+0.3
+0.7
-0.2
-0.6
carbon source
D-Alanine (C)
+0.0
-0.1
-0.4
-0.5
+0.5
lb
LB
-0.3
+0.3
+0.1
+0.3
-0.7
stress
methylglyoxal 0.009 vol%
-0.1
+0.3
+0.3
-0.2
-0.6
stress
Lomefloxacin 0.001 mM
-0.1
+0.1
+0.5
+0.1
-0.8
survival
4C survival; 360 hrs
-0.5
+0.4
-0.4
+0.0
+0.3
carbon source
L-Citrulline
-0.8
+0.0
+0.3
+0.3
+0.1
stress
Doxycycline hyclate 0.0006 mg/ml
-0.5
-0.1
+0.3
+0.4
-0.1
carbon source
D-Glucose
+0.5
+0.2
+0.4
-0.7
-0.4
stress
Thallium(I) acetate 0.016 mg/ml
-0.3
-0.3
+0.2
+0.2
+0.2
stress
Choline chloride 268.5 mM
-0.1
+0.3
-0.0
+0.4
-0.5
pH
Minimal media pH9
+0.6
-0.1
+0.1
-0.5
+0.0
anaerobic
Anaerobic, acetate donor; nitrate and iron
+0.5
+0.3
+0.1
-0.2
-0.6
stress
Thallium(I) acetate 0.008 mg/ml
-0.7
+0.0
+0.4
+0.2
+0.6
nitrogen source
Gly-DL-Asp (N)
+1.2
-0.3
+0.0
+0.1
-0.5
carbon source
L-Ornithine
+0.5
-0.1
+0.6
+0.0
-0.4
motility
outer cut, LB soft agar motility assay
+0.7
+0.4
-0.1
+0.2
-0.6
stress
Sisomicin 0.008 mg/ml
-0.3
+0.8
+0.1
+0.0
-0.2
carbon source
L-Ornithine
+0.6
+0.1
+0.4
-0.5
+0.2
carbon source
L-Citrulline
-0.6
+0.3
+0.6
+0.4
+0.5
carbon source
L-Serine (C)
+0.1
+0.5
+1.0
+0.5
+0.2
stress
Paraquat dichloride 0.032 mg/mL
-0.3
-0.3
+1.1
+0.8
+1.3
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