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Gene
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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_07725 and AO353_07730 are separated by 48 nucleotides
AO353_07730 and AO353_07735 are separated by 121 nucleotides
AO353_07735 and AO353_07740 are separated by 193 nucleotides
AO353_07740 and AO353_07745 are separated by 86 nucleotides
AO353_07725: AO353_07725 - hypothetical protein, at 1,702,889 to 1,703,290
_07725
AO353_07730: AO353_07730 - hypothetical protein, at 1,703,339 to 1,703,755
_07730
AO353_07735: AO353_07735 - AraC family transcriptional regulator, at 1,703,877 to 1,704,827
_07735
AO353_07740: AO353_07740 - ABC transporter for Carnitine, substrate-binding component
(from data)
, at 1,705,021 to 1,705,965
_07740
AO353_07745: AO353_07745 - NADPH:quinone reductase, at 1,706,052 to 1,706,939
_07745
Group
Condition
AO353
_07725
AO353
_07730
AO353
_07735
AO353
_07740
AO353
_07745
carbon source
Carnitine Hydrochloride (C)
+0.4
-0.1
-3.4
-3.1
-4.4
nitrogen source
Carnitine Hydrochloride (N)
-0.2
+0.1
-1.8
-1.7
-1.9
stress
Doxycycline hyclate 0.0006 mg/ml
-1.3
-0.0
-1.9
+0.5
+0.1
motility
outer cut, LB soft agar motility assay
-0.1
-0.8
-0.8
-0.4
-0.5
stress
Cisplatin 0.006 mg/ml
-1.2
-0.4
-0.2
-0.1
-0.2
anaerobic
Anaerobic, acetate donor; nitrate and iron
-0.2
+0.3
-1.2
-0.5
-0.5
carbon source
acetate (C)
-0.1
-0.9
-0.4
-0.4
-0.0
carbon source
L-Ornithine
-0.2
-0.3
-0.9
-0.0
-0.2
stress
Lomefloxacin 0.001 mM
-0.7
-0.7
+0.3
-0.2
-0.3
stress
Rifampicin 0.005 mg/ml
-0.4
-0.4
-0.2
-0.1
-0.4
nitrogen source
L-Arginine (N)
-0.1
-0.4
-0.3
-0.3
-0.4
carbon source
D-Glucose
-0.1
-0.4
-0.1
-0.2
-0.6
carbon source
D-Glucose
+0.1
-1.0
-0.1
+0.1
-0.5
stress
acetate 68 mM
-0.1
-0.8
-0.3
-0.2
+0.2
stress
Sisomicin 0.008 mg/ml
+0.2
-0.5
+0.0
-0.7
+0.1
nitrogen source
Thymine (N)
-0.0
-0.9
+0.3
-0.0
-0.2
carbon source
L-Aspartic Acid (C)
-0.2
+0.4
-0.4
-0.2
-0.4
stress
Cisplatin 0.006 mg/ml
-1.4
+0.8
+0.5
-0.0
-0.7
carbon source
L-Citrulline
+0.6
-0.2
-0.6
+0.1
-0.3
stress
1-ethyl-3-methylimidazolium chloride 170 mM
-0.2
-0.7
-0.1
+0.2
+0.4
carbon source
L-Phenylalanine (C)
+0.0
-0.3
+0.1
-0.9
+0.7
carbon source
L-Citrulline (C)
+0.2
-0.3
-0.8
+0.3
+0.3
carbon source
L-Leucine (C)
+0.6
-0.7
-0.0
+0.3
-0.4
lb
LB
-0.2
-0.6
+0.3
+0.4
+0.1
carbon source
L-Citrulline
+0.4
+0.5
+0.1
-0.7
-0.3
motility
outer cut, LB soft agar motility assay
-0.3
-0.4
+0.7
-0.1
+0.5
carbon source
L-Ornithine
-0.2
-0.3
+0.9
+0.1
-0.1
denitrifying
Nitrate reduction, replicate B
+0.6
+0.4
+0.2
-0.5
-0.2
carbon source
L-Valine (C)
+0.1
+0.7
+0.2
+0.2
-0.5
survival
4C survival; 672 hrs
+0.5
+0.4
+0.3
+0.2
+0.2
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