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Gene
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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_07710 and AO353_07715 are separated by 15 nucleotides
AO353_07715 and AO353_07720 are separated by 79 nucleotides
AO353_07720 and AO353_07725 are separated by 235 nucleotides
AO353_07725 and AO353_07730 are separated by 48 nucleotides
AO353_07710: AO353_07710 - DgcA Dimethylglycine demethylase subunit A
(from data)
, at 1,698,990 to 1,701,050
_07710
AO353_07715: AO353_07715 - hydrocarbon binding protein, at 1,701,066 to 1,701,596
_07715
AO353_07720: AO353_07720 - peptidase M19, at 1,701,676 to 1,702,653
_07720
AO353_07725: AO353_07725 - hypothetical protein, at 1,702,889 to 1,703,290
_07725
AO353_07730: AO353_07730 - hypothetical protein, at 1,703,339 to 1,703,755
_07730
Group
Condition
AO353
_07710
AO353
_07715
AO353
_07720
AO353
_07725
AO353
_07730
carbon source
Carnitine Hydrochloride (C)
-3.8
-3.6
-3.6
+0.4
-0.1
nitrogen source
Carnitine Hydrochloride (N)
-2.9
-2.9
-2.4
-0.2
+0.1
stress
Lomefloxacin 0.001 mM
-0.2
-0.3
+0.2
-0.7
-0.7
stress
Doxycycline hyclate 0.0006 mg/ml
+0.1
+0.1
-0.1
-1.3
-0.0
stress
1-ethyl-3-methylimidazolium acetate 30 mM
-0.3
+0.1
-0.2
-0.6
-0.3
anaerobic
Anaerobic, acetate donor; nitrate and iron
-0.4
+0.0
-0.9
-0.2
+0.3
motility
outer cut, LB soft agar motility assay
-0.2
-0.0
-0.2
-0.3
-0.4
stress
Tetracycline 0.0016 mg/ml
+0.1
-0.1
-0.2
-0.7
-0.2
stress
Rifampicin 0.005 mg/ml
+0.1
+0.1
-0.5
-0.4
-0.4
nitrogen source
Thymine (N)
+0.0
+0.2
-0.2
-0.0
-0.9
temperature
LB 30C
-0.1
-0.2
-0.1
+0.1
-0.6
stress
methylglyoxal 0.009 vol%
-0.3
+0.0
+0.2
-0.7
-0.0
stress
Cisplatin 0.006 mg/ml
+0.4
+0.3
+0.1
-1.2
-0.4
nitrogen source
Parabanic Acid (N)
+0.1
-0.2
+0.1
-0.1
-0.6
lb
LB
-0.0
+0.2
-0.1
-0.2
-0.6
carbon source
D-Glucose
+0.1
-0.1
+0.2
+0.1
-1.0
stress
Doxycycline hyclate 0.0002 mg/ml
+0.0
+0.2
+0.1
-0.5
-0.5
carbon source
acetate (C)
+0.3
+0.3
-0.2
-0.1
-0.9
carbon source
L-Leucine (C)
-0.3
+0.1
-0.1
+0.6
-0.7
stress
Cisplatin 0.006 mg/ml
+0.1
+0.3
-0.2
-1.4
+0.8
motility
outer cut, LB soft agar motility assay
+0.2
+0.2
+0.3
-0.1
-0.8
stress
nitrate 200 mM
+0.1
-0.3
+0.3
+0.3
-0.4
stress
Sisomicin 0.008 mg/ml
-0.1
+0.1
+0.4
+0.2
-0.5
carbon source
L-Citrulline
-0.1
-0.2
+0.3
+0.6
-0.2
carbon source
L-Valine (C)
-0.1
-0.1
-0.2
+0.1
+0.7
pH
Minimal media pH9
+0.4
+0.3
+0.3
-0.1
-0.3
denitrifying
Nitrate reduction, replicate B
-0.1
-0.0
-0.2
+0.6
+0.4
nitrogen source
L-Threonine (N)
+0.2
+0.2
+0.3
-0.2
+0.3
carbon source
L-Citrulline
-0.0
+0.3
+0.4
+0.4
+0.5
survival
4C survival; 672 hrs
+0.4
+0.2
+0.0
+0.5
+0.4
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