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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_07350 and AO353_07355 are separated by 107 nucleotides
AO353_07355 and AO353_07360 overlap by 10 nucleotides
AO353_07360 and AO353_07365 are separated by 74 nucleotides
AO353_07365 and AO353_07370 are separated by 36 nucleotides
AO353_07350: AO353_07350 - 2', 3'-cyclic nucleotide 2'-phosphodiesterase, at 1,615,650 to 1,616,879
_07350
AO353_07355: AO353_07355 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase, at 1,616,987 to 1,617,505
_07355
AO353_07360: AO353_07360 - dihydroneopterin aldolase, at 1,617,496 to 1,617,849
_07360
AO353_07365: AO353_07365 - glycerol-3-phosphate acyltransferase, at 1,617,924 to 1,618,493
_07365
AO353_07370: AO353_07370 - tRNA threonylcarbamoyl adenosine modification protein TsaD, at 1,618,530 to 1,619,555
_07370
Group
Condition
AO353
_07350
AO353
_07355
AO353
_07360
AO353
_07365
AO353
_07370
stress
Nickel (II) chloride 0.625 mM
N.D.
-3.0
N.D.
-1.1
N.D.
stress
Nickel (II) chloride 0.625 mM
N.D.
-2.9
N.D.
-0.5
N.D.
pH
Minimal media pH6
N.D.
-3.3
N.D.
-0.1
N.D.
pH
Minimal media pH6
N.D.
-3.4
N.D.
-0.0
N.D.
stress
Cobalt chloride 0.16 mM
N.D.
-3.3
N.D.
-0.0
N.D.
nitrogen source
L-Citrulline (N)
N.D.
-2.8
N.D.
-0.4
N.D.
motility
outer cut, LB soft agar motility assay
N.D.
-2.4
N.D.
-0.7
N.D.
temperature
LB 10C
N.D.
-3.0
N.D.
-0.1
N.D.
temperature
LB 20C
N.D.
-2.9
N.D.
-0.2
N.D.
carbon source
Fumarate (C)
N.D.
-2.8
N.D.
-0.2
N.D.
stress
Vancomycin 1 mg/ml
N.D.
-2.9
N.D.
-0.1
N.D.
pH
Minimal media pH8
N.D.
-2.6
N.D.
-0.2
N.D.
nitrogen source
L-Histidine (N)
N.D.
-2.5
N.D.
-0.3
N.D.
nitrogen source
L-Glutamic (N)
N.D.
-2.8
N.D.
+0.0
N.D.
stress
benzoic 0.58 mg/ml
N.D.
-2.7
N.D.
-0.1
N.D.
nitrogen source
L-Glutamine (N)
N.D.
-2.6
N.D.
-0.1
N.D.
temperature
LB 20C
N.D.
-2.5
N.D.
-0.3
N.D.
nitrogen source
Putrescine (N)
N.D.
-2.3
N.D.
-0.4
N.D.
carbon source
Carnitine Hydrochloride (C)
N.D.
-2.6
N.D.
-0.1
N.D.
nitrogen source
L-Histidine (N)
N.D.
-2.5
N.D.
-0.1
N.D.
stress
Cobalt chloride 0.16 mM
N.D.
-2.9
N.D.
+0.3
N.D.
temperature
LB 10C
N.D.
-2.4
N.D.
-0.2
N.D.
survival
4C survival; 192 hrs
N.D.
-2.5
N.D.
-0.1
N.D.
pH
Minimal media pH7
N.D.
-2.5
N.D.
-0.1
N.D.
nitrogen source
D-Serine (N)
N.D.
-2.6
N.D.
+0.1
N.D.
stress
Furfuryl Alcohol 0.0625 vol%
N.D.
-2.2
N.D.
-0.3
N.D.
survival
4C survival; 360 hrs
N.D.
-2.6
N.D.
+0.1
N.D.
stress
Cobalt chloride 0.32 mM
N.D.
-3.2
N.D.
+1.0
N.D.
stress
Ceftazidime 0.12 mg/ml
N.D.
-2.3
N.D.
+0.2
N.D.
stress
Cobalt chloride 0.32 mM
N.D.
-2.9
N.D.
+1.0
N.D.
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