Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E3

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500 ntAO353_07145 and AO353_07150 are separated by 67 nucleotidesAO353_07150 and AO353_07155 are separated by 167 nucleotidesAO353_07155 and AO353_07160 are separated by 135 nucleotidesAO353_07160 and AO353_07165 are separated by 34 nucleotides AO353_07145: AO353_07145 - anhydro-N-acetylmuramic acid kinase, at 1,570,694 to 1,571,785 _07145 AO353_07150: AO353_07150 - iron-sulfur cluster insertion protein ErpA, at 1,571,853 to 1,572,203 _07150 AO353_07155: AO353_07155 - N-acetyl-gamma-glutamyl-phosphate reductase, at 1,572,371 to 1,573,405 _07155 AO353_07160: AO353_07160 - hypothetical protein, at 1,573,541 to 1,573,972 _07160 AO353_07165: AO353_07165 - 2-nitropropane dioxygenase, at 1,574,007 to 1,574,975 _07165
Group Condition AO353_07145 AO353_07150 AO353_07155 AO353_07160 AO353_07165
motility outer cut, LB soft agar motility assay -2.9 N.D. -2.1 N.D. -0.1
carbon source L-Leucine (C) -1.4 N.D. -2.5 N.D. -0.4
motility outer cut, LB soft agar motility assay -2.9 N.D. -1.3 N.D. -0.0
pH Minimal media pH6 -0.2 N.D. -4.1 N.D. +0.2
carbon source Carnitine Hydrochloride (C) -1.3 N.D. -2.9 N.D. +0.1
motility outer cut, LB soft agar motility assay -2.4 N.D. -1.4 N.D. -0.0
carbon source D-Ribose (C) -0.1 N.D. -3.8 N.D. +0.0
carbon source D,L-Lactate (C) -0.1 N.D. -3.7 N.D. +0.1
pH Minimal media pH8 +0.2 N.D. -4.0 N.D. +0.1
pH Minimal media pH7 +0.3 N.D. -4.0 N.D. +0.1
nitrogen source Putrescine (N) +0.1 N.D. -3.7 N.D. +0.1
carbon source D-Glucose (C) -0.0 N.D. -3.6 N.D. +0.0
carbon source D-Gluconic Acid (C) +0.1 N.D. -3.5 N.D. -0.1
carbon source succinate (C) +0.1 N.D. -3.6 N.D. -0.1
carbon source D-Fructose (C) +0.1 N.D. -3.6 N.D. +0.1
carbon source L-Lactate (C) +0.1 N.D. -3.6 N.D. +0.0
carbon source D-Gluconic Acid (C) +0.3 N.D. -3.6 N.D. -0.0
pH Minimal media pH6 -0.2 N.D. -3.3 N.D. +0.2
carbon source a-Ketoglutaric (C) +0.3 N.D. -3.6 N.D. +0.0
carbon source Fumarate (C) +0.5 N.D. -3.7 N.D. +0.1
carbon source a-Ketoglutaric (C) +0.6 N.D. -3.5 N.D. -0.2
nitrogen source Urea (N) +0.3 N.D. -3.1 N.D. -0.2
carbon source D-Lactate (C) +0.3 N.D. -3.3 N.D. -0.0
pH Minimal media pH8 +0.3 N.D. -3.3 N.D. +0.1
pH Minimal media pH9 +0.6 N.D. -3.2 N.D. -0.0
nitrogen source Adenosine (N) +0.9 N.D. -3.3 N.D. +0.0
carbon source propionate (C) +0.8 N.D. -3.1 N.D. -0.1
stress Doxycycline hyclate 0.0002 mg/ml +2.7 N.D. -1.3 N.D. +0.2
stress Rifampicin 0.005 mg/ml +3.5 N.D. -1.5 N.D. -0.1
stress Doxycycline hyclate 0.0006 mg/ml +4.6 N.D. +0.1 N.D. +0.3
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