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Gene
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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_06760 and AO353_06765 are separated by 161 nucleotides
AO353_06765 and AO353_06770 are separated by 56 nucleotides
AO353_06770 and AO353_06775 overlap by 8 nucleotides
AO353_06775 and AO353_06780 are separated by 364 nucleotides
AO353_06760: AO353_06760 - hypothetical protein, at 1,501,830 to 1,502,348
_06760
AO353_06765: AO353_06765 - hypothetical protein, at 1,502,510 to 1,503,208
_06765
AO353_06770: AO353_06770 - NAD-dependent dehydratase, at 1,503,265 to 1,504,194
_06770
AO353_06775: AO353_06775 - dTDP-4-dehydrorhamnose reductase, at 1,504,187 to 1,505,071
_06775
AO353_06780: AO353_06780 - DNA repair helicase Lhr
(from data)
, at 1,505,436 to 1,509,752
_06780
Group
Condition
AO353
_06760
AO353
_06765
AO353
_06770
AO353
_06775
AO353
_06780
stress
Doxycycline hyclate 0.0006 mg/ml
-1.0
+0.3
N.D.
-1.4
-0.2
stress
Rifampicin 0.005 mg/ml
+0.1
-0.2
N.D.
-2.2
+0.1
stress
1-ethyl-3-methylimidazolium acetate 30 mM
-0.2
-0.5
N.D.
-1.1
-0.2
carbon source
acetate (C)
-0.2
-0.1
N.D.
-1.7
+0.3
carbon source
acetate (C)
+0.1
+0.1
N.D.
-2.0
+0.0
stress
Lomefloxacin 0.001 mM
-0.3
-0.6
N.D.
-0.9
-0.0
carbon source
propionate (C)
+0.4
+0.3
N.D.
-2.7
+0.8
motility
outer cut, LB soft agar motility assay
+0.3
-0.3
N.D.
-1.1
-0.1
stress
Doxycycline hyclate 0.0002 mg/ml
+0.2
-0.1
N.D.
-1.2
-0.1
carbon source
a-Ketoglutaric (C)
-0.0
-0.2
N.D.
-1.0
+0.1
carbon source
L-Alanine (C)
+0.3
-0.9
N.D.
-0.1
-0.2
survival
4C survival; 672 hrs
+0.3
-0.5
N.D.
-0.6
-0.1
carbon source
D-Glucose
+0.3
-0.7
N.D.
-0.3
+0.1
anaerobic
N1B4wt with N2E3m denitrifying replicate C
-0.1
-1.3
N.D.
+0.9
+0.1
motility
outer cut, LB soft agar motility assay
+0.4
-0.1
N.D.
-1.0
+0.4
carbon source
L-Ornithine
+0.7
-0.1
N.D.
-0.9
+0.0
stress
Paraquat dichloride 0.032 mg/mL
-0.3
-0.2
N.D.
+0.7
-0.2
stress
Cisplatin 0.006 mg/ml
+0.5
-0.4
N.D.
+1.6
-1.7
carbon source
propionate (C)
+0.3
+0.4
N.D.
-1.4
+0.8
stress
Cholin acetate 19 mM
+0.1
-0.5
N.D.
+0.8
-0.1
carbon source
Uridine (C)
+0.2
-0.2
N.D.
+0.6
-0.3
carbon source
L-Valine (C)
+0.4
-0.9
N.D.
+1.1
-0.2
pH
Minimal media pH9
+0.0
-0.5
N.D.
+0.9
+0.1
stress
Cisplatin 0.006 mg/ml
-0.0
-0.4
N.D.
+2.3
-1.4
stress
methylglyoxal 0.009 vol%
-0.4
-0.3
N.D.
+1.7
-0.4
carbon source
L-Ornithine
+0.7
-0.5
N.D.
+0.5
+0.0
carbon source
L-Citrulline
+0.1
-0.6
N.D.
+1.1
+0.1
stress
nitrate 200 mM
-0.3
+0.4
N.D.
+1.0
-0.1
pH
Minimal media pH9
+0.8
-0.3
N.D.
+0.7
-0.0
stress
Fusidic 0.125 mg/ml
-0.1
+0.0
N.D.
+2.1
-0.0
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