Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E3

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500 ntAO353_06760 and AO353_06765 are separated by 161 nucleotidesAO353_06765 and AO353_06770 are separated by 56 nucleotidesAO353_06770 and AO353_06775 overlap by 8 nucleotidesAO353_06775 and AO353_06780 are separated by 364 nucleotides AO353_06760: AO353_06760 - hypothetical protein, at 1,501,830 to 1,502,348 _06760 AO353_06765: AO353_06765 - hypothetical protein, at 1,502,510 to 1,503,208 _06765 AO353_06770: AO353_06770 - NAD-dependent dehydratase, at 1,503,265 to 1,504,194 _06770 AO353_06775: AO353_06775 - dTDP-4-dehydrorhamnose reductase, at 1,504,187 to 1,505,071 _06775 AO353_06780: AO353_06780 - DNA repair helicase Lhr (from data), at 1,505,436 to 1,509,752 _06780
Group Condition AO353_06760 AO353_06765 AO353_06770 AO353_06775 AO353_06780
stress Doxycycline hyclate 0.0006 mg/ml -1.0 +0.3 N.D. -1.4 -0.2
stress Rifampicin 0.005 mg/ml +0.1 -0.2 N.D. -2.2 +0.1
stress 1-ethyl-3-methylimidazolium acetate 30 mM -0.2 -0.5 N.D. -1.1 -0.2
carbon source acetate (C) -0.2 -0.1 N.D. -1.7 +0.3
carbon source acetate (C) +0.1 +0.1 N.D. -2.0 +0.0
stress Lomefloxacin 0.001 mM -0.3 -0.6 N.D. -0.9 -0.0
carbon source propionate (C) +0.4 +0.3 N.D. -2.7 +0.8
motility outer cut, LB soft agar motility assay +0.3 -0.3 N.D. -1.1 -0.1
stress Doxycycline hyclate 0.0002 mg/ml +0.2 -0.1 N.D. -1.2 -0.1
carbon source a-Ketoglutaric (C) -0.0 -0.2 N.D. -1.0 +0.1
carbon source L-Alanine (C) +0.3 -0.9 N.D. -0.1 -0.2
survival 4C survival; 672 hrs +0.3 -0.5 N.D. -0.6 -0.1
carbon source D-Glucose +0.3 -0.7 N.D. -0.3 +0.1
anaerobic N1B4wt with N2E3m denitrifying replicate C -0.1 -1.3 N.D. +0.9 +0.1
motility outer cut, LB soft agar motility assay +0.4 -0.1 N.D. -1.0 +0.4
carbon source L-Ornithine +0.7 -0.1 N.D. -0.9 +0.0
stress Paraquat dichloride 0.032 mg/mL -0.3 -0.2 N.D. +0.7 -0.2
stress Cisplatin 0.006 mg/ml +0.5 -0.4 N.D. +1.6 -1.7
carbon source propionate (C) +0.3 +0.4 N.D. -1.4 +0.8
stress Cholin acetate 19 mM +0.1 -0.5 N.D. +0.8 -0.1
carbon source Uridine (C) +0.2 -0.2 N.D. +0.6 -0.3
carbon source L-Valine (C) +0.4 -0.9 N.D. +1.1 -0.2
pH Minimal media pH9 +0.0 -0.5 N.D. +0.9 +0.1
stress Cisplatin 0.006 mg/ml -0.0 -0.4 N.D. +2.3 -1.4
stress methylglyoxal 0.009 vol% -0.4 -0.3 N.D. +1.7 -0.4
carbon source L-Ornithine +0.7 -0.5 N.D. +0.5 +0.0
carbon source L-Citrulline +0.1 -0.6 N.D. +1.1 +0.1
stress nitrate 200 mM -0.3 +0.4 N.D. +1.0 -0.1
pH Minimal media pH9 +0.8 -0.3 N.D. +0.7 -0.0
stress Fusidic 0.125 mg/ml -0.1 +0.0 N.D. +2.1 -0.0
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