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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_06085 and AO353_06090 are separated by 138 nucleotides
AO353_06090 and AO353_06095 are separated by 109 nucleotides
AO353_06095 and AO353_06100 are separated by 11 nucleotides
AO353_06100 and AO353_06105 are separated by 614 nucleotides
AO353_06085: AO353_06085 - omega amino acid--pyruvate aminotransferase, at 1,357,404 to 1,358,753
_06085
AO353_06090: AO353_06090 - LysR family transcriptional regulator, at 1,358,892 to 1,359,812
_06090
AO353_06095: AO353_06095 - TetR family transcriptional regulator, at 1,359,922 to 1,360,572
_06095
AO353_06100: AO353_06100 - thymidylate synthase, at 1,360,584 to 1,361,165
_06100
AO353_06105: AO353_06105 - short-chain dehydrogenase, at 1,361,780 to 1,362,466
_06105
Group
Condition
AO353
_06085
AO353
_06090
AO353
_06095
AO353
_06100
AO353
_06105
nitrogen source
Uridine (N)
-3.8
-3.9
+0.1
-0.2
-0.0
nitrogen source
Thymine (N)
-2.7
-2.3
-0.0
-0.0
+0.2
carbon source
Uridine (C)
-1.1
-1.2
+0.1
-1.4
-0.1
carbon source
L-Citrulline
-0.1
-0.1
-0.5
-1.5
-0.2
motility
outer cut, LB soft agar motility assay
+0.2
-0.7
-0.5
-1.5
+0.2
anaerobic
Anaerobic, acetate donor; nitrate and iron
+0.3
+0.0
+0.1
-3.1
+0.4
carbon source
L-Citrulline
+0.2
-0.1
-0.4
-2.2
+0.4
carbon source
L-Phenylalanine (C)
-0.0
-0.3
+0.0
-1.6
+0.1
survival
4C survival; 672 hrs
-0.4
-0.3
+0.3
-1.5
+0.1
carbon source
propionate (C)
+0.0
+0.1
-0.4
-1.2
-0.2
stress
Doxycycline hyclate 0.0006 mg/ml
-0.1
-0.2
-0.1
-1.3
-0.1
stress
Cobalt chloride 0.32 mM
+0.1
-0.2
+0.0
-1.1
-0.4
carbon source
L-Isoleucine (C)
-0.3
+0.1
-0.0
-1.5
+0.4
carbon source
pyruvate (C)
+0.1
-0.1
+0.1
-1.3
-0.2
nitrogen source
L-Histidine (N)
-0.1
+0.3
-0.1
-1.4
+0.1
nitrogen source
Carnitine Hydrochloride (N)
+0.3
-0.1
+0.0
-1.0
-0.3
motility
outer cut, LB soft agar motility assay
-0.0
+0.3
-0.2
-0.6
-0.5
carbon source
L-Citrulline
-0.1
+0.2
-0.1
-1.1
+0.2
carbon source
L-Leucine (C)
+0.2
-0.5
-0.3
-0.7
+0.3
nitrogen source
L-Glutamine (N)
-0.0
+0.3
+0.0
-1.2
+0.1
stress
Cisplatin 0.006 mg/ml
-0.6
-0.0
+0.5
+0.3
-0.5
stress
acetate 34 mM
+0.0
-0.4
-0.2
+0.8
+0.2
denitrifying
Nitrate reduction, replicate C
+0.2
-0.2
+0.1
+0.9
-0.3
pH
Minimal media pH9
-0.6
-0.6
+0.3
+1.5
+0.2
carbon source
L-Tryptophan (C)
-0.3
+0.2
-0.1
+0.9
+0.3
temperature
LB 20C
-0.0
+0.2
+0.1
+1.1
-0.2
pH
Minimal media pH8
-0.1
+0.1
-0.1
+1.3
+0.1
stress
Thallium(I) acetate 0.008 mg/ml
-0.2
+0.0
+0.1
+1.4
-0.1
carbon source
L-Citrulline
+0.1
-0.1
+0.1
+1.4
+0.7
carbon source
D-Glucose
+0.1
-0.2
+0.4
+1.4
+0.5
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