Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E3

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500 ntAO353_06085 and AO353_06090 are separated by 138 nucleotidesAO353_06090 and AO353_06095 are separated by 109 nucleotidesAO353_06095 and AO353_06100 are separated by 11 nucleotidesAO353_06100 and AO353_06105 are separated by 614 nucleotides AO353_06085: AO353_06085 - omega amino acid--pyruvate aminotransferase, at 1,357,404 to 1,358,753 _06085 AO353_06090: AO353_06090 - LysR family transcriptional regulator, at 1,358,892 to 1,359,812 _06090 AO353_06095: AO353_06095 - TetR family transcriptional regulator, at 1,359,922 to 1,360,572 _06095 AO353_06100: AO353_06100 - thymidylate synthase, at 1,360,584 to 1,361,165 _06100 AO353_06105: AO353_06105 - short-chain dehydrogenase, at 1,361,780 to 1,362,466 _06105
Group Condition AO353_06085 AO353_06090 AO353_06095 AO353_06100 AO353_06105
nitrogen source Uridine (N) -3.8 -3.9 +0.1 -0.2 -0.0
nitrogen source Thymine (N) -2.7 -2.3 -0.0 -0.0 +0.2
carbon source Uridine (C) -1.1 -1.2 +0.1 -1.4 -0.1
carbon source L-Citrulline -0.1 -0.1 -0.5 -1.5 -0.2
motility outer cut, LB soft agar motility assay +0.2 -0.7 -0.5 -1.5 +0.2
anaerobic Anaerobic, acetate donor; nitrate and iron +0.3 +0.0 +0.1 -3.1 +0.4
carbon source L-Citrulline +0.2 -0.1 -0.4 -2.2 +0.4
carbon source L-Phenylalanine (C) -0.0 -0.3 +0.0 -1.6 +0.1
survival 4C survival; 672 hrs -0.4 -0.3 +0.3 -1.5 +0.1
carbon source propionate (C) +0.0 +0.1 -0.4 -1.2 -0.2
stress Doxycycline hyclate 0.0006 mg/ml -0.1 -0.2 -0.1 -1.3 -0.1
stress Cobalt chloride 0.32 mM +0.1 -0.2 +0.0 -1.1 -0.4
carbon source L-Isoleucine (C) -0.3 +0.1 -0.0 -1.5 +0.4
carbon source pyruvate (C) +0.1 -0.1 +0.1 -1.3 -0.2
nitrogen source L-Histidine (N) -0.1 +0.3 -0.1 -1.4 +0.1
nitrogen source Carnitine Hydrochloride (N) +0.3 -0.1 +0.0 -1.0 -0.3
motility outer cut, LB soft agar motility assay -0.0 +0.3 -0.2 -0.6 -0.5
carbon source L-Citrulline -0.1 +0.2 -0.1 -1.1 +0.2
carbon source L-Leucine (C) +0.2 -0.5 -0.3 -0.7 +0.3
nitrogen source L-Glutamine (N) -0.0 +0.3 +0.0 -1.2 +0.1
stress Cisplatin 0.006 mg/ml -0.6 -0.0 +0.5 +0.3 -0.5
stress acetate 34 mM +0.0 -0.4 -0.2 +0.8 +0.2
denitrifying Nitrate reduction, replicate C +0.2 -0.2 +0.1 +0.9 -0.3
pH Minimal media pH9 -0.6 -0.6 +0.3 +1.5 +0.2
carbon source L-Tryptophan (C) -0.3 +0.2 -0.1 +0.9 +0.3
temperature LB 20C -0.0 +0.2 +0.1 +1.1 -0.2
pH Minimal media pH8 -0.1 +0.1 -0.1 +1.3 +0.1
stress Thallium(I) acetate 0.008 mg/ml -0.2 +0.0 +0.1 +1.4 -0.1
carbon source L-Citrulline +0.1 -0.1 +0.1 +1.4 +0.7
carbon source D-Glucose +0.1 -0.2 +0.4 +1.4 +0.5
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