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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_05970 and AO353_05975 overlap by 4 nucleotides
AO353_05975 and AO353_05980 are separated by 280 nucleotides
AO353_05980 and AO353_05985 are separated by 249 nucleotides
AO353_05985 and AO353_05990 are separated by 10 nucleotides
AO353_05970: AO353_05970 - Tryptophan 2,3-dioxygenase (EC 1.13.11.11)
(from data)
, at 1,326,547 to 1,327,413
_05970
AO353_05975: AO353_05975 - Kynurenine formamidase, bacterial (EC 3.5.1.9)
(from data)
, at 1,327,410 to 1,328,075
_05975
AO353_05980: AO353_05980 - cupin, at 1,328,356 to 1,328,700
_05980
AO353_05985: AO353_05985 - 3-hydroxyisobutyrate dehydrogenase (EC 1.1.1.31)
(from data)
, at 1,328,950 to 1,329,834
_05985
AO353_05990: AO353_05990 - Methylmalonate-semialdehyde dehydrogenase (EC 1.2.1.27)
(from data)
, at 1,329,845 to 1,331,368
_05990
Group
Condition
AO353
_05970
AO353
_05975
AO353
_05980
AO353
_05985
AO353
_05990
carbon source
L-Tryptophan (C)
-3.0
-3.3
-2.5
-0.4
-0.3
carbon source
L-Valine (C)
-0.1
-0.5
+0.1
-3.7
-3.5
nitrogen source
L-Tryptophan (N)
-3.3
-3.2
-0.5
-0.4
-0.0
carbon source
L-Isoleucine (C)
+0.1
-0.5
-0.1
-1.4
-0.5
stress
Cisplatin 0.006 mg/ml
-0.3
-0.5
-0.3
-0.4
+0.1
carbon source
L-Aspartic Acid (C)
-0.2
-0.2
-0.1
-0.3
-0.4
carbon source
L-Ornithine
-0.6
-0.0
+0.1
-0.6
-0.1
carbon source
L-Leucine (C)
-0.1
-0.2
+0.0
-0.5
-0.3
carbon source
L-Phenylalanine (C)
-0.1
-0.0
-0.2
-0.7
-0.2
pH
Minimal media pH9
-0.4
-0.1
+0.1
-0.5
-0.0
nitrogen source
Gly-Glu (N)
-0.1
-0.2
-0.5
-0.3
+0.4
stress
copper (II) chloride 2.5 mM
-0.3
-0.1
-0.2
-0.2
+0.3
carbon source
D-Serine (C)
-0.3
+0.2
-0.5
-0.2
+0.3
stress
Cisplatin 0.006 mg/ml
-0.2
-0.3
-0.2
-0.4
+0.4
stress
Nickel (II) chloride 0.625 mM
-0.6
+0.1
-0.3
+0.1
+0.3
carbon source
D-Glucose
-0.3
+0.2
+0.2
-0.4
+0.2
motility
outer cut, LB soft agar motility assay
+0.1
+0.1
-0.2
-0.5
+0.4
carbon source
L-Citrulline
+0.4
-0.0
-0.1
-0.5
+0.4
radiation
Ionizing radiation, 126.196 Gy,
+0.4
+0.3
-0.2
-0.1
-0.2
nitrogen source
L-Glutamine (N)
+0.4
-0.2
-0.1
+0.4
-0.3
carbon source
D-Alanine (C)
+0.1
+0.2
+0.2
-0.5
+0.2
carbon source
D-Glucose
-0.2
+0.3
-0.2
+0.2
+0.2
carbon source
acetate (C)
+0.4
+0.5
-0.8
+0.2
+0.3
anaerobic
N1B4wt with N2E3m denitrifying replicate C
-0.0
+0.3
+0.2
-0.3
+0.3
motility
outer cut, LB soft agar motility assay
+0.7
+0.1
+0.2
-0.2
-0.2
motility
outer cut, LB soft agar motility assay
+0.6
+0.2
+0.2
+0.3
-0.4
stress
Choline chloride 268.5 mM
+0.4
+0.1
+0.2
+0.2
+0.3
stress
Doxycycline hyclate 0.0006 mg/ml
+0.9
+0.1
+0.4
+0.2
-0.3
anaerobic
Anaerobic, acetate donor; nitrate and iron
+0.2
+0.5
-0.1
+0.4
+0.3
carbon source
L-Citrulline
+0.2
+0.1
+0.2
+0.4
+0.5
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