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Gene
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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_05710 and AO353_05715 overlap by 1 nucleotides
AO353_05715 and AO353_05720 overlap by 4 nucleotides
AO353_05720 and AO353_05725 are separated by 163 nucleotides
AO353_05725 and AO353_05730 are separated by 496 nucleotides
AO353_05710: AO353_05710 - (Fe-S)-binding protein, at 1,261,089 to 1,262,543
_05710
AO353_05715: AO353_05715 - lactate utilization protein B/C, at 1,262,543 to 1,263,214
_05715
AO353_05720: AO353_05720 - 4Fe-4S ferredoxin, at 1,263,211 to 1,266,021
_05720
AO353_05725: AO353_05725 - tmRNA, at 1,266,185 to 1,266,460
_05725
AO353_05730: AO353_05730 - hypothetical protein, at 1,266,957 to 1,267,229
_05730
Group
Condition
AO353
_05710
AO353
_05715
AO353
_05720
AO353
_05725
AO353
_05730
carbon source
D-Lactate (C)
-3.8
-4.6
-4.1
-0.1
-0.3
carbon source
D-Lactate (C)
-3.8
-4.4
-3.7
+0.1
-0.1
carbon source
D,L-Lactate (C)
-3.6
-4.4
-0.6
-0.1
-0.2
carbon source
D,L-Lactate (C)
-3.3
-4.1
-0.7
-0.2
-0.0
carbon source
L-Lactate (C)
-3.5
-4.3
+0.2
+0.1
+0.1
carbon source
L-Lactate (C)
-3.4
-4.3
+0.3
+0.1
+0.1
carbon source
Glycerol (C)
-2.1
-2.6
-2.2
-0.5
+0.1
carbon source
Glycerol (C)
-2.0
-2.4
-2.3
-0.3
+0.2
stress
Fusidic 0.125 mg/ml
-0.3
-0.2
-0.5
-1.1
-0.2
stress
copper (II) chloride 2.5 mM
+0.0
-0.1
+0.2
-0.5
-1.5
carbon source
L-Valine (C)
-0.8
-0.0
-0.4
-0.1
-0.5
carbon source
m-Inositol (C)
-0.2
-0.2
-0.0
-0.6
-0.4
motility
outer cut, LB soft agar motility assay
-0.3
-0.4
-0.3
-0.2
-0.3
carbon source
L-Phenylalanine (C)
-0.1
-0.3
-0.0
+0.1
-1.1
stress
Spectinomycin 0.2 mg/ml
-0.2
-0.1
-0.2
-0.8
-0.0
anaerobic
Anaerobic, acetate donor; nitrate and iron
-0.1
+0.2
-0.2
-0.0
-1.1
carbon source
acetate (C)
-0.3
-0.2
+0.2
-0.7
-0.4
nitrogen source
Uridine (N)
-0.3
-0.2
+0.1
-0.1
-0.5
nitrogen source
D-Glucosamine Hydrochloride (N)
-0.2
-0.3
-0.1
-0.7
+0.2
carbon source
acetate (C)
-0.5
-0.4
+0.0
+0.3
-0.4
pH
Minimal media pH9
-0.2
-0.3
-0.0
+0.4
-0.5
survival
4C survival; 672 hrs
+0.1
-0.1
+0.3
-0.2
-0.7
stress
Sisomicin 0.008 mg/ml
-0.2
-0.6
-0.1
-0.1
+0.5
stress
Spectinomycin 0.1 mg/ml
-0.4
-0.3
-0.0
-0.3
+0.5
motility
outer cut, LB soft agar motility assay
-0.4
-0.2
-0.2
+0.4
+0.3
stress
Cisplatin 0.006 mg/ml
-0.2
-0.2
+0.5
-0.2
+0.1
carbon source
L-Citrulline
-0.1
+0.3
+0.5
-0.4
+0.2
nitrogen source
Parabanic Acid (N)
+0.1
-0.3
-0.1
+0.6
+0.1
carbon source
D-Glucose
+0.2
-0.1
-0.0
+0.5
+0.9
stress
nitrate 200 mM
+0.4
+0.3
+0.1
+0.5
+0.1
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