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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_05395 and AO353_05400 are separated by 10 nucleotides
AO353_05400 and AO353_05405 are separated by 10 nucleotides
AO353_05405 and AO353_05410 overlap by 1 nucleotides
AO353_05410 and AO353_05415 are separated by 120 nucleotides
AO353_05395: AO353_05395 - peptide ABC transporter permease, at 1,189,568 to 1,190,578
_05395
AO353_05400: AO353_05400 - peptide transporter, at 1,190,589 to 1,191,500
_05400
AO353_05405: AO353_05405 - peptide ABC transporter ATP-binding protein, at 1,191,511 to 1,192,479
_05405
AO353_05410: AO353_05410 - peptide ABC transporter ATP-binding protein, at 1,192,479 to 1,193,450
_05410
AO353_05415: AO353_05415 - hypothetical protein, at 1,193,571 to 1,193,801
_05415
Group
Condition
AO353
_05395
AO353
_05400
AO353
_05405
AO353
_05410
AO353
_05415
nitrogen source
Gly-Glu (N)
-2.6
-2.7
-3.9
-2.7
-0.4
nitrogen source
Gly-DL-Asp (N)
-1.8
-2.8
-3.2
-2.5
-1.2
stress
methylglyoxal 0.008 vol%
+0.2
+0.1
+0.0
+0.1
-4.0
motility
outer cut, LB soft agar motility assay
-0.4
-0.6
-0.3
-0.2
-1.8
stress
Nickel (II) chloride 0.625 mM
-0.8
-0.0
-0.4
-0.2
-0.7
nitrogen source
D-Alanine (N)
+0.1
-0.1
-0.2
-0.2
-1.3
carbon source
L-Citrulline
-0.1
+0.1
+0.1
-0.3
-1.4
stress
Dimethyl Sulfoxide 7.5 vol%
-0.1
+0.1
-0.2
+0.0
-1.4
carbon source
L-Citrulline
-0.1
-0.6
-0.7
-0.3
+0.4
lb
LB
-0.6
+0.3
+0.2
+0.1
-1.3
survival
4C survival; 672 hrs
-0.3
-0.5
-0.9
-0.5
+0.9
stress
Sisomicin 0.008 mg/ml
+0.1
+0.3
+0.1
+0.2
-1.4
carbon source
L-Citrulline
-0.5
-0.1
-0.5
-0.2
+0.8
pH
Minimal media pH9
-0.4
-0.3
-0.5
+0.1
+0.6
motility
outer cut, LB soft agar motility assay
-0.3
-0.5
+0.2
-0.5
+0.5
pH
Minimal media pH9
-0.0
-0.4
-0.6
+0.0
+0.6
carbon source
L-Leucine (C)
+0.5
+0.2
-0.2
-0.0
-0.9
stress
Doxycycline hyclate 0.0006 mg/ml
+0.0
+0.3
-0.3
+0.5
-0.6
survival
4C survival; 360 hrs
-0.3
-0.4
-0.2
-0.0
+0.9
carbon source
L-Citrulline
-0.4
+0.5
-0.4
-0.3
+0.8
nitrogen source
L-Histidine (N)
-0.3
-0.2
+0.0
-0.2
+0.9
carbon source
D-Glucose
+0.2
-0.3
-0.1
-0.2
+1.1
stress
Chloride 500 mM
-0.2
+0.2
-0.2
-0.1
+1.0
anaerobic
Anaerobic, acetate donor; nitrate and iron
+0.5
-0.0
-0.3
+0.1
+0.8
stress
methylglyoxal 0.009 vol%
+0.6
+0.3
+0.3
+0.4
-0.2
stress
Cisplatin 0.006 mg/ml
+0.1
+1.2
+0.2
-0.0
+0.1
carbon source
propionate (C)
+0.1
+0.9
+0.3
+0.5
-0.1
nitrogen source
L-Serine (N)
+0.4
+0.2
+0.1
+0.1
+0.9
carbon source
propionate (C)
+0.5
+0.6
+0.6
+0.7
-0.2
denitrifying
Nitrate reduction, replicate B
+0.2
+0.2
+0.3
+0.1
+1.5
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