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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_05185 and AO353_05190 are separated by 12 nucleotides
AO353_05190 and AO353_05195 are separated by 2 nucleotides
AO353_05195 and AO353_05200 are separated by 15 nucleotides
AO353_05200 and AO353_05205 are separated by 218 nucleotides
AO353_05185: AO353_05185 - hypothetical protein, at 1,141,138 to 1,141,446
_05185
AO353_05190: AO353_05190 - PTS fructose transporter subunit IIA, at 1,141,459 to 1,141,923
_05190
AO353_05195: AO353_05195 - glmZ(sRNA)-inactivating NTPase, at 1,141,926 to 1,142,783
_05195
AO353_05200: AO353_05200 - phosphocarrier protein HPr, at 1,142,799 to 1,143,071
_05200
AO353_05205: AO353_05205 - peptidase PmbA, at 1,143,290 to 1,144,636
_05205
Group
Condition
AO353
_05185
AO353
_05190
AO353
_05195
AO353
_05200
AO353
_05205
anaerobic
N1B4wt with N2E3m denitrifying replicate C
-1.9
N.D.
-1.9
N.D.
-1.2
carbon source
propionate (C)
-1.2
N.D.
-2.7
N.D.
-0.6
carbon source
propionate (C)
-0.7
N.D.
-2.8
N.D.
-0.4
stress
Cisplatin 0.006 mg/ml
-0.7
N.D.
-0.7
N.D.
-1.7
motility
outer cut, LB soft agar motility assay
-0.8
N.D.
-1.1
N.D.
-1.1
carbon source
L-Valine (C)
-0.5
N.D.
-2.4
N.D.
-0.1
motility
outer cut, LB soft agar motility assay
+0.3
N.D.
-1.8
N.D.
-0.9
motility
outer cut, LB soft agar motility assay
-0.3
N.D.
-1.2
N.D.
-0.8
pH
Minimal media pH9
-1.0
N.D.
-0.8
N.D.
-0.6
denitrifying
Nitrate reduction, replicate C
-0.9
N.D.
-0.2
N.D.
-1.2
pH
Minimal media pH9
-0.7
N.D.
-0.8
N.D.
-0.6
carbon source
L-Leucine (C)
+0.1
N.D.
-1.7
N.D.
-0.4
carbon source
Ethanol (C)
-0.5
N.D.
-1.4
N.D.
+0.1
carbon source
Ethanol (C)
-0.4
N.D.
-1.2
N.D.
+0.0
carbon source
L-Citrulline (C)
-0.2
N.D.
-1.4
N.D.
-0.0
carbon source
m-Inositol (C)
+0.2
N.D.
-1.7
N.D.
-0.1
carbon source
m-Inositol (C)
+0.2
N.D.
-1.5
N.D.
-0.3
carbon source
acetate (C)
+0.3
N.D.
-1.3
N.D.
-0.2
stress
Cisplatin 0.006 mg/ml
+0.4
N.D.
-0.1
N.D.
-1.6
carbon source
L-Proline (C)
+0.5
N.D.
-1.1
N.D.
-0.1
stress
Sisomicin 0.008 mg/ml
+0.9
N.D.
-0.5
N.D.
-0.7
stress
methylglyoxal 0.009 vol%
-1.0
N.D.
+0.6
N.D.
+0.3
stress
methylglyoxal 0.008 vol%
-0.4
N.D.
+1.4
N.D.
-0.4
stress
Doxycycline hyclate 0.0002 mg/ml
-0.8
N.D.
+2.2
N.D.
-0.0
stress
Lomefloxacin 0.001 mM
-0.0
N.D.
+1.5
N.D.
-0.1
stress
Tetracycline 0.0016 mg/ml
-0.5
N.D.
+2.5
N.D.
-0.2
anaerobic
Anaerobic, acetate donor; nitrate and iron
+0.5
N.D.
+2.1
N.D.
-0.4
stress
Rifampicin 0.005 mg/ml
-0.4
N.D.
+2.7
N.D.
+0.3
carbon source
L-Serine (C)
+0.3
N.D.
+2.5
N.D.
+0.2
stress
Doxycycline hyclate 0.0006 mg/ml
-1.2
N.D.
+4.7
N.D.
-0.2
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