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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_04870 and AO353_04875 are separated by 21 nucleotides
AO353_04875 and AO353_04880 overlap by 1 nucleotides
AO353_04880 and AO353_04885 are separated by 137 nucleotides
AO353_04885 and AO353_04890 are separated by 38 nucleotides
AO353_04870: AO353_04870 - AraC family transcriptional regulator, at 1,075,648 to 1,076,523
_04870
AO353_04875: AO353_04875 - hypothetical protein, at 1,076,545 to 1,077,597
_04875
AO353_04880: AO353_04880 - TonB-dependent receptor, at 1,077,597 to 1,080,038
_04880
AO353_04885: AO353_04885 - multifunctional 2',3'-cyclic-nucleotide 2'-phosphodiesterase/5'-nucleotidase/3'-nucleotidase, at 1,080,176 to 1,081,876
_04885
AO353_04890: AO353_04890 - biopolymer transporter ExbB, at 1,081,915 to 1,082,559
_04890
Group
Condition
AO353
_04870
AO353
_04875
AO353
_04880
AO353
_04885
AO353
_04890
anaerobic
Anaerobic, acetate donor; nitrate and iron
+0.3
-0.2
+0.1
-0.1
-1.3
carbon source
D-Glucose
+0.3
-0.3
-0.1
-0.5
-0.5
carbon source
L-Alanine (C)
+0.2
+0.3
-0.0
-0.9
-0.2
carbon source
L-Valine (C)
-0.2
-0.7
+0.3
+0.1
-0.1
survival
4C survival; 672 hrs
+0.3
-0.3
+0.1
-0.7
+0.2
denitrifying
Nitrate reduction, replicate C
-1.2
+0.0
-0.1
+0.4
+0.4
motility
outer cut, LB soft agar motility assay
+0.4
-0.3
+0.1
+0.0
-0.6
nitrogen source
Parabanic Acid (N)
-0.3
+0.4
-0.1
+0.0
-0.4
stress
Cisplatin 0.006 mg/ml
+0.3
+0.0
-0.4
-0.6
+0.5
carbon source
pyruvate (C)
-0.6
-0.4
+0.0
+0.2
+0.5
stress
Lomefloxacin 0.001 mM
-0.6
+0.1
+0.2
-0.4
+0.5
carbon source
L-Leucine (C)
-0.2
-0.4
+0.1
+0.2
+0.2
pH
Minimal media pH9
+0.1
-0.4
+0.0
-0.3
+0.5
denitrifying
Nitrate reduction, replicate B
-0.9
+0.3
+0.0
+0.4
+0.2
carbon source
L-Citrulline
-0.5
+0.5
-0.0
-0.0
+0.1
stress
Thallium(I) acetate 0.008 mg/ml
+0.3
+0.3
+0.0
-0.2
-0.3
motility
outer cut, LB soft agar motility assay
+0.4
+0.6
-0.0
+0.2
-1.1
stress
Doxycycline hyclate 0.0006 mg/ml
+0.4
+0.4
-0.0
-0.6
+0.1
carbon source
m-Inositol (C)
-0.4
+0.2
+0.1
+0.1
+0.4
carbon source
L-Citrulline (C)
+0.3
+0.2
+0.1
+0.3
-0.5
pH
Minimal media pH7
+0.3
-0.4
+0.1
-0.1
+0.5
lb
LB
+0.3
+0.3
+0.0
+0.2
-0.4
carbon source
L-Citrulline
+0.4
+0.5
-0.2
+0.0
-0.0
pH
Minimal media pH9
+0.2
+0.5
+0.1
+0.6
-0.3
stress
Sisomicin 0.008 mg/ml
+0.5
+0.5
+0.1
-0.1
+0.2
anaerobic
N1B4wt with N2E3m denitrifying replicate C
+0.3
+0.4
+0.0
+0.5
+0.3
carbon source
L-Ornithine
+3.2
+0.5
+0.1
-0.3
+0.5
carbon source
L-Ornithine
+3.5
+0.9
+0.3
-0.1
-0.4
carbon source
L-Ornithine
+6.5
+0.8
-0.0
+0.2
+0.0
carbon source
L-Ornithine
+7.4
+0.8
+0.1
-0.2
+0.2
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