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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_04695 and AO353_04700 overlap by 4 nucleotides
AO353_04700 and AO353_04705 overlap by 1 nucleotides
AO353_04705 and AO353_04710 are separated by 11 nucleotides
AO353_04710 and AO353_04715 are separated by 8 nucleotides
AO353_04695: AO353_04695 - alginate biosynthesis protein, at 1,014,136 to 1,015,515
_04695
AO353_04700: AO353_04700 - transcriptional regulator, at 1,015,512 to 1,016,996
_04700
AO353_04705: AO353_04705 - poly(beta-D-mannuronate) C5 epimerase, at 1,016,996 to 1,018,615
_04705
AO353_04710: AO353_04710 - alginate O-acetyltransferase, at 1,018,627 to 1,020,075
_04710
AO353_04715: AO353_04715 - poly(beta-D-mannuronate) lyase, at 1,020,084 to 1,021,208
_04715
Group
Condition
AO353
_04695
AO353
_04700
AO353
_04705
AO353
_04710
AO353
_04715
anaerobic
Anaerobic, acetate donor; nitrate and iron
-0.3
-1.0
-0.3
-0.8
-0.5
stress
1-ethyl-3-methylimidazolium chloride 170 mM
-0.5
-0.5
-0.3
-0.3
-0.7
stress
Thallium(I) acetate 0.016 mg/ml
-0.1
-0.9
-0.1
-0.1
-0.5
stress
Doxycycline hyclate 0.0002 mg/ml
-0.3
-0.2
-0.1
-0.4
-0.4
stress
Cobalt chloride 0.32 mM
-0.3
-0.2
+0.0
-0.3
-0.7
stress
nitrate 200 mM
-0.3
-0.5
-0.1
-0.3
-0.1
carbon source
Cytidine (C)
-0.2
-0.3
-0.2
-0.2
-0.4
stress
Paraquat dichloride 0.032 mg/mL
-0.1
-0.1
-0.1
-0.4
-0.5
stress
methylglyoxal 0.009 vol%
-0.5
-0.2
+0.0
-0.3
-0.3
stress
Cisplatin 0.006 mg/ml
-0.4
-0.4
+0.1
-0.1
-0.2
stress
Lomefloxacin 0.001 mM
-0.2
-0.2
-0.3
-0.3
-0.1
carbon source
L-Ornithine
-0.1
-0.4
-0.1
-0.5
-0.0
carbon source
acetate (C)
-0.4
-0.2
+0.0
-0.5
-0.0
stress
Sisomicin 0.008 mg/ml
-0.2
-0.4
+0.1
-0.4
-0.1
stress
Cisplatin 0.006 mg/ml
+0.0
-0.9
-0.1
+0.0
-0.1
stress
Thallium(I) acetate 0.008 mg/ml
-0.1
-0.6
+0.1
-0.1
-0.3
stress
Spectinomycin 0.15 mg/ml
-0.1
-0.7
-0.1
-0.0
+0.1
carbon source
acetate (C)
-0.4
-0.3
-0.0
+0.2
-0.2
stress
Furfuryl Alcohol 0.125 vol%
-0.2
+0.2
-0.2
-0.1
-0.3
stress
1-ethyl-3-methylimidazolium lysine 10.5 mM
-0.4
-0.6
-0.1
+0.0
+0.2
carbon source
L-Serine (C)
-0.2
-0.2
+0.2
-0.2
-0.3
stress
Doxycycline hyclate 0.0006 mg/ml
-0.1
+0.2
+0.1
-0.2
-0.5
carbon source
L-Aspartic Acid (C)
+0.3
-0.3
-0.1
+0.3
-0.2
carbon source
L-Citrulline
-0.5
+0.4
-0.2
-0.2
+0.5
pH
Minimal media pH9
-0.1
-0.4
+0.2
+0.1
+0.3
motility
outer cut, LB soft agar motility assay
+0.6
-0.3
-0.2
+0.1
-0.1
motility
outer cut, LB soft agar motility assay
+0.8
-0.2
+0.0
-0.2
+0.1
carbon source
L-Ornithine
-0.1
+0.4
+0.1
-0.2
+0.4
carbon source
L-Citrulline
+0.1
+0.3
+0.3
-0.2
+0.4
stress
Cholin acetate 10 mM
+0.1
+0.2
+0.2
+0.2
+0.4
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