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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_04455 and AO353_04460 are separated by 24 nucleotides
AO353_04460 and AO353_04465 are separated by 28 nucleotides
AO353_04465 and AO353_04470 are separated by 240 nucleotides
AO353_04470 and AO353_04475 are separated by 190 nucleotides
AO353_04455: AO353_04455 - Glucosamine-6-phosphate deaminase [isomerizing], alternative (EC 3.5.99.6)
(from data)
, at 957,107 to 958,117
_04455
AO353_04460: AO353_04460 - N-acetylglucosamine-specific PTS system, I, HPr, and IIA components (nagF)
(from data)
, at 958,142 to 960,658
_04460
AO353_04465: AO353_04465 - N-acetylglucosamine-specific PTS system, IIBC components (nagE)
(from data)
, at 960,687 to 962,402
_04465
AO353_04470: AO353_04470 - hypothetical protein, at 962,643 to 962,942
_04470
AO353_04475: AO353_04475 - hypothetical protein, at 963,133 to 963,522
_04475
Group
Condition
AO353
_04455
AO353
_04460
AO353
_04465
AO353
_04470
AO353
_04475
nitrogen source
NAG (N)
-3.7
-3.1
-2.6
-0.2
+0.5
carbon source
D-Glucosamine Hydrochloride (C)
-3.7
-0.0
+0.1
+0.2
+0.3
pH
Minimal media pH9
-0.9
-0.3
-0.0
-0.4
-0.5
motility
outer cut, LB soft agar motility assay
-0.1
-0.1
+0.1
-0.0
-1.4
nitrogen source
L-Histidine (N)
-0.3
-0.4
-0.2
-0.0
-0.4
carbon source
L-Phenylalanine (C)
-0.8
-0.0
+0.2
-0.0
-0.7
stress
Lomefloxacin 0.001 mM
+0.2
-0.6
+0.2
+0.3
-1.3
motility
outer cut, LB soft agar motility assay
-0.2
-0.4
+0.2
+0.1
-0.9
stress
Doxycycline hyclate 0.0006 mg/ml
-0.8
-0.1
-0.1
-0.2
+0.0
carbon source
L-Malic (C)
-0.2
-0.1
+0.0
+0.1
-1.0
nitrogen source
D-Glucosamine Hydrochloride (N)
-1.7
+0.3
+0.0
+0.1
+0.3
carbon source
D-Trehalose (C)
-0.2
+0.1
-0.0
-0.0
-0.9
nitrogen source
L-Lysine (N)
+0.1
-0.1
-0.2
+0.2
-0.8
stress
Cholin acetate 38 mM
-0.1
+0.3
-0.2
+0.1
-0.8
carbon source
L-Aspartic Acid (C)
-0.2
-0.0
-0.0
+0.3
-0.7
stress
Paraquat dichloride 0.032 mg/mL
-0.1
+0.4
-0.1
+0.0
-1.0
carbon source
L-Ornithine
-0.3
-0.2
+0.2
+0.1
-0.4
stress
1-ethyl-3-methylimidazolium acetate 30 mM
+0.0
+0.3
-0.0
-0.0
-0.8
carbon source
L-Glutamic (C)
-0.2
+0.4
-0.2
+0.1
-0.5
anaerobic
Anaerobic, acetate donor; nitrate and iron
-0.8
+0.2
-0.1
+0.5
-0.3
carbon source
L-Histidine (C)
-0.1
+0.4
-0.0
+0.0
-0.7
carbon source
propionate (C)
-0.2
+0.2
+0.3
+0.2
-0.3
carbon source
acetate (C)
+0.1
+0.3
+0.0
+0.4
-0.4
stress
Cisplatin 0.006 mg/ml
+0.1
-0.0
-0.4
-0.4
+1.1
carbon source
L-Tryptophan (C)
-0.0
+0.1
+0.3
-0.3
+0.8
carbon source
Uridine (C)
+0.2
-0.0
+0.2
+0.2
+0.7
carbon source
L-Citrulline
+0.1
+0.2
+0.2
-0.1
+0.9
carbon source
L-Citrulline
-0.0
+0.4
+0.1
+0.1
+1.0
carbon source
L-Ornithine
+0.4
+0.3
+0.1
-0.1
+0.9
survival
4C survival; 672 hrs
+0.4
+1.3
+1.4
-0.1
-1.0
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