Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E3

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500 ntAO353_04210 and AO353_04215 are separated by 320 nucleotidesAO353_04215 and AO353_04220 are separated by 42 nucleotidesAO353_04220 and AO353_04225 overlap by 4 nucleotidesAO353_04225 and AO353_04230 overlap by 4 nucleotides AO353_04210: AO353_04210 - peptide chain release factor 2, at 903,742 to 904,623 _04210 AO353_04215: AO353_04215 - diguanylate cyclase, at 904,944 to 905,945 _04215 AO353_04220: AO353_04220 - chemotaxis protein CheY, at 905,988 to 906,998 _04220 AO353_04225: AO353_04225 - chemotaxis protein CheA, at 906,995 to 909,289 _04225 AO353_04230: AO353_04230 - chemotaxis protein CheW, at 909,286 to 909,969 _04230
Group Condition AO353_04210 AO353_04215 AO353_04220 AO353_04225 AO353_04230
motility outer cut, LB soft agar motility assay N.D. +0.1 -1.5 -0.6 -1.8
carbon source propionate (C) N.D. -0.3 -2.0 -0.2 -1.0
carbon source L-Tryptophan (C) N.D. -0.0 -3.2 -0.1 +0.2
carbon source L-Phenylalanine (C) N.D. -0.3 -1.8 +0.1 -0.8
carbon source L-Valine (C) N.D. +0.2 -2.0 +0.1 -0.8
carbon source L-Citrulline N.D. +0.2 -1.3 +0.1 -1.5
carbon source L-Alanine (C) N.D. +0.1 -1.1 -0.2 -1.4
stress nitrate 200 mM N.D. +0.2 -2.0 -0.3 -0.4
motility outer cut, LB soft agar motility assay N.D. +0.3 -1.2 -0.3 -1.0
denitrifying Nitrate reduction, replicate C N.D. -0.1 -1.3 +0.1 -0.9
carbon source L-Citrulline N.D. +0.3 -1.9 +0.1 -0.5
nitrogen source L-tyrosine (N) N.D. -0.2 -2.3 -0.1 +0.6
carbon source L-Leucine (C) N.D. +0.2 -1.7 +0.1 -0.6
nitrogen source NAG (N) N.D. +0.2 -2.1 +0.1 -0.1
carbon source L-Citrulline N.D. +0.4 -2.1 +0.2 -0.4
carbon source L-Citrulline N.D. +0.7 -2.6 -0.1 +0.2
carbon source L-Lactate (C) N.D. -0.0 -1.9 -0.2 +0.3
nitrogen source Thymine (N) N.D. -0.1 -2.0 +0.2 +0.2
carbon source Tween 20 (C) N.D. +0.1 -2.0 +0.1 +0.3
nitrogen source Gly-DL-Asp (N) N.D. +0.1 -1.0 +0.4 -1.0
nitrogen source Parabanic Acid (N) N.D. +0.1 -2.0 +0.2 +0.6
carbon source L-Histidine (C) N.D. +0.1 -1.7 +0.1 +0.6
stress Doxycycline hyclate 0.0006 mg/ml N.D. +0.2 +0.6 +0.5 -1.6
stress acetate 68 mM N.D. +0.1 +1.8 +0.3 +0.2
stress Cholin acetate 19 mM N.D. +0.1 +2.4 +0.5 -0.5
stress acetate 17 mM N.D. +0.1 +2.2 +0.3 +0.4
stress 1-ethyl-3-methylimidazolium acetate 20 mM N.D. +0.0 +2.8 +0.6 -0.2
stress 1-ethyl-3-methylimidazolium acetate 15 mM N.D. +0.0 +2.7 +0.6 -0.1
stress acetate 34 mM N.D. +0.0 +2.4 +0.5 +0.3
stress Cholin acetate 38 mM N.D. +0.1 +2.4 +0.6 +0.3
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