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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_03485 and AO353_03490 are separated by 182 nucleotides
AO353_03490 and AO353_03495 are separated by 495 nucleotides
AO353_03495 and AO353_03500 are separated by 9 nucleotides
AO353_03500 and AO353_03505 are separated by 185 nucleotides
AO353_03485: AO353_03485 - acetyl-CoA carboxylase, at 745,300 to 745,656
_03485
AO353_03490: AO353_03490 - Fis family transcriptional regulator, at 745,839 to 747,347
_03490
AO353_03495: AO353_03495 - glycine cleavage system protein H, at 747,843 to 748,226
_03495
AO353_03500: AO353_03500 - Glycine dehydrogenase (aminomethyl-transferring) (EC 1.4.4.2)
(from data)
, at 748,236 to 751,088
_03500
AO353_03505: AO353_03505 - serine dehydratase, at 751,274 to 752,650
_03505
Group
Condition
AO353
_03485
AO353
_03490
AO353
_03495
AO353
_03500
AO353
_03505
nitrogen source
Glycine (N)
+0.2
-3.1
-2.3
-3.8
-1.0
nitrogen source
Glycine (N)
-0.2
-2.6
-2.5
-3.1
-0.8
nitrogen source
L-Threonine (N)
+0.1
-2.1
-2.7
-2.8
-0.8
nitrogen source
L-Threonine (N)
+0.1
-2.0
-1.6
-2.8
-0.7
nitrogen source
Gly-Glu (N)
+0.1
-1.0
-1.4
-2.4
+0.0
pH
Minimal media pH9
+0.1
-0.7
-0.6
-1.7
-0.1
pH
Minimal media pH9
+0.3
-0.3
-0.4
-1.4
-0.1
carbon source
L-Phenylalanine (C)
-0.1
-0.5
-0.7
-0.2
+0.1
nitrogen source
Gly-DL-Asp (N)
+0.2
-0.3
-0.5
-0.6
-0.2
stress
Cisplatin 0.006 mg/ml
-0.3
-0.3
-0.5
+0.1
-0.0
carbon source
L-Ornithine
+0.0
-0.3
-0.8
-0.1
+0.1
stress
methylglyoxal 0.009 vol%
+0.2
-0.7
-0.4
+0.0
-0.1
carbon source
L-Ornithine
-0.0
-0.4
-0.6
-0.1
+0.3
nitrogen source
Parabanic Acid (N)
+0.4
+0.2
-0.2
-0.5
-0.7
stress
Lomefloxacin 0.001 mM
-0.0
-0.1
-0.7
+0.3
-0.2
anaerobic
Anaerobic, acetate donor; nitrate and iron
+0.3
-0.5
-0.3
+0.3
-0.2
carbon source
L-Serine (C)
+0.0
-0.1
+1.2
-0.7
-0.8
carbon source
acetate (C)
+0.3
-0.3
-0.2
-0.2
+0.3
carbon source
propionate (C)
+0.2
-0.1
+0.5
-0.4
-0.2
motility
outer cut, LB soft agar motility assay
-0.1
+0.4
-0.7
+0.4
+0.4
stress
Fusidic 0.125 mg/ml
+0.4
-0.2
+0.5
+0.1
+0.1
carbon source
L-Citrulline
+0.3
-0.3
+0.3
+0.2
+0.3
stress
Cholin acetate 38 mM
+0.0
-0.0
+0.7
-0.2
+0.4
carbon source
succinate (C)
+0.1
+0.0
+0.3
+0.2
+0.5
stress
Sisomicin 0.008 mg/ml
+0.5
+0.3
+0.0
+0.1
+0.5
motility
outer cut, LB soft agar motility assay
+0.3
+1.0
+0.2
+0.5
-0.1
carbon source
L-Alanine (C)
-0.1
+0.5
+0.4
+0.5
+0.6
denitrifying
Nitrate reduction, replicate B
-0.1
+0.7
+0.8
+1.0
-0.1
stress
Doxycycline hyclate 0.0006 mg/ml
+0.6
+0.4
+0.4
+0.4
+0.6
denitrifying
Nitrate reduction, replicate C
-0.1
+0.7
+0.8
+0.8
+0.2
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