Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E3

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500 ntAO353_02850 and AO353_02855 are separated by 15 nucleotidesAO353_02855 and AO353_02860 are separated by 3 nucleotidesAO353_02860 and AO353_02865 are separated by 249 nucleotidesAO353_02865 and AO353_02870 are separated by 45 nucleotides AO353_02850: AO353_02850 - flagellar biosynthesis protein FlgD, at 607,323 to 608,051 _02850 AO353_02855: AO353_02855 - flagellar basal-body rod protein FlgC, at 608,067 to 608,510 _02855 AO353_02860: AO353_02860 - flagellar biosynthesis protein FlgB, at 608,514 to 608,921 _02860 AO353_02865: AO353_02865 - chemotaxis protein, at 609,171 to 609,998 _02865 AO353_02870: AO353_02870 - chemotaxis protein CheW, at 610,044 to 610,976 _02870
Group Condition AO353_02850 AO353_02855 AO353_02860 AO353_02865 AO353_02870
motility outer cut, LB soft agar motility assay -0.8 -2.7 -3.5 -3.0 -0.3
motility outer cut, LB soft agar motility assay -1.9 -2.7 -2.8 -2.1 -0.5
motility outer cut, LB soft agar motility assay -1.4 -2.4 -3.0 -2.0 -0.5
survival 4C survival; 360 hrs -1.0 -1.7 -2.9 -1.6 -0.1
survival 4C survival; 192 hrs -0.7 -1.3 -2.0 -1.0 -0.4
survival 4C survival; 672 hrs -0.9 -1.5 -1.4 -1.3 -0.0
stress Cobalt chloride 0.32 mM -0.2 -0.4 -0.4 +0.1 -0.5
pH Minimal media pH9 +0.2 -0.0 -0.6 -0.1 -0.2
stress Cholin acetate 10 mM -0.1 +0.2 -0.5 +0.2 +0.3
carbon source L-Ornithine +0.0 +0.6 -0.4 +0.1 -0.3
carbon source L-Citrulline -0.1 +0.4 -0.2 +0.4 -0.3
stress Benzalkonium Chloride 0.012 mg/ml -0.2 +0.6 -0.4 +0.2 +0.1
carbon source L-Valine (C) -0.5 +0.1 +0.4 +0.4 +0.0
stress nitrate 200 mM +0.1 -0.4 +0.4 +0.2 +0.1
carbon source L-Ornithine +0.3 +0.0 -0.1 +0.5 -0.3
carbon source Trisodium citrate (C) -0.3 +0.3 +0.5 +0.0 -0.0
stress Rifampicin 0.005 mg/ml -0.2 +0.7 +0.6 -0.3 -0.1
stress Cholin acetate 38 mM -0.2 +0.2 +0.3 +0.3 +0.2
stress Thallium(I) acetate 0.016 mg/ml +0.0 +0.2 +0.5 +0.4 -0.3
stress Thallium(I) acetate 0.008 mg/ml +0.2 +0.4 +0.7 +0.0 -0.4
nitrogen source Parabanic Acid (N) +0.0 +0.6 +0.4 +0.2 -0.2
stress methylglyoxal 0.009 vol% +0.2 -0.2 +0.2 +0.3 +0.5
nitrogen source Inosine (N) +0.3 +0.5 +0.5 -0.1 -0.1
carbon source L-Ornithine +0.4 +0.2 +0.6 +0.2 -0.2
stress 1-ethyl-3-methylimidazolium acetate 20 mM +0.0 +0.6 +0.3 +0.3 +0.0
carbon source a-Ketoglutaric (C) +0.3 +0.5 +0.7 +0.2 -0.1
denitrifying Nitrate reduction, replicate C -0.1 +0.6 +1.0 +0.4 -0.2
stress Doxycycline hyclate 0.0006 mg/ml +0.2 +0.5 +0.7 +0.1 +0.3
carbon source D-Glucosamine Hydrochloride (C) +0.4 +0.9 +0.9 -0.0 -0.1
nitrogen source Urea (N) +0.4 +0.7 +0.9 +0.0 +0.3
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