Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E3

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500 ntAO353_02835 and AO353_02840 are separated by 730 nucleotidesAO353_02840 and AO353_02845 are separated by 172 nucleotidesAO353_02845 and AO353_02850 are separated by 31 nucleotidesAO353_02850 and AO353_02855 are separated by 15 nucleotides AO353_02835: AO353_02835 - chemotaxis protein CheY, at 601,437 to 602,162 _02835 AO353_02840: AO353_02840 - ribonucleotide-diphosphate reductase subunit alpha, at 602,893 to 605,787 _02840 AO353_02845: AO353_02845 - flagellar biosynthesis protein FlgE, at 605,960 to 607,291 _02845 AO353_02850: AO353_02850 - flagellar biosynthesis protein FlgD, at 607,323 to 608,051 _02850 AO353_02855: AO353_02855 - flagellar basal-body rod protein FlgC, at 608,067 to 608,510 _02855
Group Condition AO353_02835 AO353_02840 AO353_02845 AO353_02850 AO353_02855
motility outer cut, LB soft agar motility assay -0.4 N.D. -2.8 -1.9 -2.7
motility outer cut, LB soft agar motility assay -0.2 N.D. -2.8 -1.4 -2.4
motility outer cut, LB soft agar motility assay +0.6 N.D. -2.5 -0.8 -2.7
survival 4C survival; 360 hrs -0.1 N.D. -2.2 -1.0 -1.7
survival 4C survival; 672 hrs -0.2 N.D. -2.0 -0.9 -1.5
survival 4C survival; 192 hrs +0.1 N.D. -1.0 -0.7 -1.3
anaerobic Anaerobic, acetate donor; nitrate and iron -2.5 N.D. -0.1 -0.1 -0.1
carbon source butyrate (C) -1.2 N.D. +0.0 -0.0 -0.1
carbon source L-Valine (C) -0.8 N.D. +0.1 -0.5 +0.1
carbon source acetate (C) -0.8 N.D. -0.1 -0.2 +0.1
stress methylglyoxal 0.009 vol% -0.8 N.D. +0.3 +0.2 -0.2
stress Cisplatin 0.006 mg/ml -0.8 N.D. +0.2 +0.3 +0.1
stress methylglyoxal 0.008 vol% -0.4 N.D. +0.2 +0.1 +0.2
denitrifying Nitrate reduction, replicate C -0.4 N.D. +0.1 -0.1 +0.6
carbon source L-Citrulline -0.4 N.D. +0.4 -0.1 +0.4
stress Rifampicin 0.005 mg/ml -0.5 N.D. +0.3 -0.2 +0.7
stress Cholin acetate 19 mM -0.3 N.D. +0.4 +0.2 +0.3
carbon source L-Ornithine -0.2 N.D. +0.2 +0.0 +0.6
nitrogen source Parabanic Acid (N) -0.4 N.D. +0.4 +0.0 +0.6
stress Benzalkonium Chloride 0.012 mg/ml +0.3 N.D. +0.0 -0.2 +0.6
stress Doxycycline hyclate 0.0006 mg/ml -0.3 N.D. +0.4 +0.2 +0.5
stress 1-ethyl-3-methylimidazolium acetate 20 mM +0.3 N.D. +0.2 +0.0 +0.6
carbon source L-Tryptophan (C) +0.2 N.D. +0.5 +0.1 +0.4
pH Minimal media pH6 +0.5 N.D. +0.3 +0.1 +0.3
carbon source a-Ketoglutaric (C) +0.0 N.D. +0.4 +0.3 +0.5
pH Minimal media pH9 +0.7 N.D. +0.2 +0.3 +0.3
pH Minimal media pH6 +0.3 N.D. +0.5 +0.3 +0.4
nitrogen source Inosine (N) +0.2 N.D. +0.6 +0.3 +0.5
nitrogen source Urea (N) -0.0 N.D. +0.5 +0.4 +0.7
carbon source D-Glucosamine Hydrochloride (C) +0.1 N.D. +0.8 +0.4 +0.9
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