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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_02525 and AO353_02530 are separated by 91 nucleotides
AO353_02530 and AO353_02535 are separated by 84 nucleotides
AO353_02535 and AO353_02540 are separated by 106 nucleotides
AO353_02540 and AO353_02545 are separated by 84 nucleotides
AO353_02525: AO353_02525 - molecular chaperone, at 537,739 to 538,059
_02525
AO353_02530: AO353_02530 - glycerol acyltransferase, at 538,151 to 540,025
_02530
AO353_02535: AO353_02535 - C4-dicarboxylate ABC transporter, at 540,110 to 541,261
_02535
AO353_02540: AO353_02540 - cupin, at 541,368 to 541,679
_02540
AO353_02545: AO353_02545 - MFS transporter, at 541,764 to 542,948
_02545
Group
Condition
AO353
_02525
AO353
_02530
AO353
_02535
AO353
_02540
AO353
_02545
stress
Sisomicin 0.008 mg/ml
-1.0
-0.2
-0.5
-0.2
-0.3
stress
Doxycycline hyclate 0.0006 mg/ml
-0.9
-0.0
-0.7
-0.3
+0.1
stress
Thallium(I) acetate 0.008 mg/ml
-0.8
+0.1
-0.2
-0.0
-0.1
stress
1-ethyl-3-methylimidazolium lysine 10.5 mM
-0.4
-0.3
-0.2
-0.3
+0.1
nitrogen source
Parabanic Acid (N)
-0.4
+0.1
+0.1
-0.6
-0.2
carbon source
acetate (C)
+0.5
-0.1
+0.2
-0.6
-0.7
carbon source
D-Glucose
+0.1
+0.2
-0.0
-0.3
-0.5
stress
Tetracycline 0.0016 mg/ml
+0.5
-0.3
-0.1
-0.1
-0.5
pH
Minimal media pH9
-0.3
-0.2
+0.1
+0.2
-0.3
carbon source
L-Citrulline
-0.8
+0.3
+0.2
-0.1
-0.0
stress
1-ethyl-3-methylimidazolium acetate 30 mM
-0.5
-0.0
-0.2
+0.2
+0.2
carbon source
L-Citrulline (C)
-0.1
-0.1
-0.0
+0.4
-0.4
carbon source
D-Glucose
-0.4
+0.1
+0.2
-0.3
+0.1
stress
methylglyoxal 0.009 vol%
+0.2
+0.2
-0.5
+0.3
-0.3
carbon source
acetate (C)
+0.7
-0.2
+0.0
-0.2
-0.3
stress
Paraquat dichloride 0.032 mg/mL
+0.2
+0.0
-0.7
+0.2
+0.3
stress
1-ethyl-3-methylimidazolium acetate 20 mM
-0.3
-0.1
-0.2
+0.2
+0.4
stress
Spectinomycin 0.15 mg/ml
-0.4
+0.2
+0.2
+0.2
-0.0
carbon source
L-Alanine (C)
+0.6
+0.0
-0.0
-0.0
-0.5
motility
outer cut, LB soft agar motility assay
-0.1
+0.3
-0.1
+0.4
-0.3
motility
outer cut, LB soft agar motility assay
-0.8
+0.3
+0.4
+0.3
+0.3
stress
Cisplatin 0.006 mg/ml
+0.3
+0.2
+0.2
-0.3
+0.1
pH
Minimal media pH7
+0.5
+0.1
-0.2
+0.2
+0.0
carbon source
L-Ornithine
+0.9
-0.0
-0.3
+0.4
-0.1
stress
Cisplatin 0.006 mg/ml
+0.5
+0.0
+0.3
-0.1
+0.2
motility
outer cut, LB soft agar motility assay
+0.4
-0.0
+0.1
+0.3
+0.2
carbon source
L-Proline (C)
+0.9
+0.2
+0.2
-0.0
-0.0
carbon source
L-Ornithine
+0.0
+0.5
+0.3
+0.0
+0.4
anaerobic
Anaerobic, acetate donor; nitrate and iron
+0.3
+0.3
+0.5
+0.0
+0.4
carbon source
L-Ornithine
+0.4
+0.1
+0.5
+0.7
+0.2
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