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Gene
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Cofit
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_02485 and AO353_02490 are separated by 28 nucleotides
AO353_02490 and AO353_02495 are separated by 140 nucleotides
AO353_02495 and AO353_02500 are separated by 19 nucleotides
AO353_02500 and AO353_02505 are separated by 186 nucleotides
AO353_02485: AO353_02485 - hypothetical protein, at 531,776 to 533,593
_02485
AO353_02490: AO353_02490 - catalase, at 533,622 to 534,758
_02490
AO353_02495: AO353_02495 - tRNA-Asp, at 534,899 to 534,975
_02495
AO353_02500: AO353_02500 - tRNA-Val, at 534,995 to 535,070
_02500
AO353_02505: AO353_02505 - tRNA-Asp, at 535,257 to 535,333
_02505
Group
Condition
AO353
_02485
AO353
_02490
AO353
_02495
AO353
_02500
AO353
_02505
stress
methylglyoxal 0.008 vol%
-0.0
-0.4
N.D.
N.D.
N.D.
stress
Cobalt chloride 0.32 mM
-0.0
-0.3
N.D.
N.D.
N.D.
stress
1-ethyl-3-methylimidazolium acetate 30 mM
+0.0
-0.3
N.D.
N.D.
N.D.
motility
outer cut, LB soft agar motility assay
+0.1
-0.3
N.D.
N.D.
N.D.
carbon source
L-Aspartic Acid (C)
+0.1
-0.2
N.D.
N.D.
N.D.
stress
Nickel (II) chloride 0.625 mM
+0.1
-0.2
N.D.
N.D.
N.D.
lb
LB
+0.1
-0.2
N.D.
N.D.
N.D.
stress
nitrate 200 mM
+0.1
-0.2
N.D.
N.D.
N.D.
stress
Doxycycline hyclate 0.0006 mg/ml
+0.2
-0.3
N.D.
N.D.
N.D.
stress
Cisplatin 0.006 mg/ml
-0.2
+0.2
N.D.
N.D.
N.D.
carbon source
L-Citrulline
-0.3
+0.4
N.D.
N.D.
N.D.
carbon source
D-Trehalose (C)
+0.2
-0.1
N.D.
N.D.
N.D.
carbon source
L-Citrulline (C)
+0.2
-0.1
N.D.
N.D.
N.D.
carbon source
L-Ornithine
-0.1
+0.2
N.D.
N.D.
N.D.
stress
Cisplatin 0.006 mg/ml
-0.2
+0.4
N.D.
N.D.
N.D.
nitrogen source
Gly-DL-Asp (N)
+0.0
+0.3
N.D.
N.D.
N.D.
pH
Minimal media pH9
+0.0
+0.3
N.D.
N.D.
N.D.
survival
4C survival; 672 hrs
+0.2
+0.2
N.D.
N.D.
N.D.
motility
outer cut, LB soft agar motility assay
+0.1
+0.2
N.D.
N.D.
N.D.
pH
Minimal media pH9
+0.3
-0.0
N.D.
N.D.
N.D.
carbon source
Cytidine (C)
+0.1
+0.2
N.D.
N.D.
N.D.
survival
4C survival; 360 hrs
+0.1
+0.2
N.D.
N.D.
N.D.
carbon source
L-Citrulline
+0.2
+0.2
N.D.
N.D.
N.D.
stress
methylglyoxal 0.009 vol%
+0.2
+0.2
N.D.
N.D.
N.D.
stress
Vancomycin 1 mg/ml
+0.1
+0.3
N.D.
N.D.
N.D.
stress
Sisomicin 0.008 mg/ml
+0.3
+0.1
N.D.
N.D.
N.D.
carbon source
L-Tryptophan (C)
+0.1
+0.3
N.D.
N.D.
N.D.
carbon source
D-Glucose
+0.1
+0.4
N.D.
N.D.
N.D.
carbon source
L-Citrulline
+0.3
+0.3
N.D.
N.D.
N.D.
anaerobic
Anaerobic, acetate donor; nitrate and iron
+0.4
+0.5
N.D.
N.D.
N.D.
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