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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_01645 and AO353_01650 are separated by 159 nucleotides
AO353_01650 and AO353_01655 are separated by 131 nucleotides
AO353_01655 and AO353_01660 are separated by 136 nucleotides
AO353_01660 and AO353_01665 are separated by 232 nucleotides
AO353_01645: AO353_01645 - hypothetical protein, at 345,123 to 345,881
_01645
AO353_01650: AO353_01650 - chemotaxis protein, at 346,041 to 348,008
_01650
AO353_01655: AO353_01655 - GntR family transcriptional regulator, at 348,140 to 349,546
_01655
AO353_01660: AO353_01660 - short-chain dehydrogenase, at 349,683 to 350,444
_01660
AO353_01665: AO353_01665 - NADP-dependent oxidoreductase, at 350,677 to 351,681
_01665
Group
Condition
AO353
_01645
AO353
_01650
AO353
_01655
AO353
_01660
AO353
_01665
motility
outer cut, LB soft agar motility assay
-2.3
+0.0
-0.0
+0.1
-0.6
motility
outer cut, LB soft agar motility assay
-1.7
+0.0
-0.2
+0.0
-0.3
survival
4C survival; 360 hrs
-1.4
+0.3
-0.0
-0.1
-0.2
survival
4C survival; 192 hrs
-1.1
+0.0
-0.0
-0.2
-0.1
stress
Cisplatin 0.006 mg/ml
-0.5
-0.1
-0.1
-0.4
+0.1
pH
Minimal media pH9
-0.2
-0.2
-0.2
-0.4
-0.1
anaerobic
Anaerobic, acetate donor; nitrate and iron
-0.3
-0.5
+0.3
-0.2
-0.2
motility
outer cut, LB soft agar motility assay
-0.8
+0.6
-0.3
-0.0
-0.1
carbon source
L-Glutamine (C)
-0.4
-0.2
+0.1
+0.1
-0.2
survival
4C survival; 672 hrs
-1.2
+0.7
+0.1
-0.1
+0.0
carbon source
acetate (C)
-0.2
+0.6
-0.1
-0.3
-0.3
stress
methylglyoxal 0.009 vol%
-0.5
+0.1
+0.2
+0.2
-0.2
carbon source
L-Leucine (C)
-0.4
+0.1
+0.3
+0.0
-0.2
carbon source
D-Trehalose (C)
-0.2
+0.4
-0.2
-0.0
-0.1
denitrifying
Nitrate reduction, replicate B
-0.3
+0.3
+0.3
-0.4
-0.0
stress
Paraquat dichloride 0.032 mg/mL
+0.3
-0.4
+0.0
-0.2
+0.1
carbon source
L-Citrulline
-0.4
+0.4
-0.2
+0.2
+0.1
carbon source
acetate (C)
-0.4
+0.4
+0.1
+0.2
-0.3
stress
Spectinomycin 0.2 mg/ml
+0.4
-0.1
+0.1
+0.1
-0.3
nitrogen source
Adenosine (N)
-0.0
+0.4
-0.1
+0.2
-0.2
carbon source
L-Ornithine
-0.2
+0.4
-0.1
+0.2
-0.2
stress
Thallium(I) acetate 0.008 mg/ml
+0.3
+0.1
-0.1
-0.2
+0.2
carbon source
propionate (C)
-0.3
+0.5
+0.0
+0.1
-0.1
carbon source
L-Citrulline
-0.7
+0.5
+0.2
+0.0
+0.3
stress
perchlorate 75 mM
+0.2
+0.3
+0.1
-0.1
-0.2
stress
methylglyoxal 0.008 vol%
-0.1
+0.4
+0.1
-0.2
+0.2
stress
Cobalt chloride 0.16 mM
+0.2
+0.2
+0.2
+0.1
-0.3
carbon source
L-Proline (C)
-0.1
+0.0
+0.2
+0.5
-0.1
carbon source
L-Ornithine
-0.2
+0.2
+0.1
+0.1
+0.3
carbon source
L-Aspartic Acid (C)
+0.3
+0.4
+0.0
+0.2
-0.2
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