Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E3

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500 ntAO353_01295 and AO353_01300 are separated by 45 nucleotidesAO353_01300 and AO353_01305 are separated by 17 nucleotidesAO353_01305 and AO353_01310 are separated by 248 nucleotidesAO353_01310 and AO353_01315 are separated by 140 nucleotides AO353_01295: AO353_01295 - alpha/beta hydrolase, at 281,846 to 282,718 _01295 AO353_01300: AO353_01300 - proteophosphoglycan precursor, at 282,764 to 283,324 _01300 AO353_01305: AO353_01305 - hypothetical protein, at 283,342 to 283,947 _01305 AO353_01310: AO353_01310 - radical SAM protein, at 284,196 to 285,164 _01310 AO353_01315: AO353_01315 - TetR family transcriptional regulator, at 285,305 to 285,943 _01315
Group Condition AO353_01295 AO353_01300 AO353_01305 AO353_01310 AO353_01315
motility outer cut, LB soft agar motility assay +0.1 -0.3 -2.8 +0.5 -0.2
motility outer cut, LB soft agar motility assay +0.1 -0.1 -2.3 +0.4 -0.7
survival 4C survival; 672 hrs +0.2 +0.1 -1.4 -0.2 -1.3
survival 4C survival; 360 hrs +0.1 +0.1 -0.6 -0.2 -0.9
motility outer cut, LB soft agar motility assay +0.4 +0.4 -2.5 +0.7 -0.4
anaerobic N1B4wt with N2E3m denitrifying replicate C -0.0 -0.4 -0.1 -1.2 +0.4
carbon source Tween 20 (C) +0.1 +0.1 +0.0 -0.2 -1.3
stress Rifampicin 0.005 mg/ml -0.1 +0.1 -0.5 -0.4 -0.2
stress Lomefloxacin 0.001 mM -0.2 +0.1 -0.1 -0.6 -0.4
nitrogen source Adenosine (N) -0.0 +0.1 +0.1 -0.8 -0.4
carbon source L-Citrulline -0.4 +0.3 +0.1 -0.1 -0.7
carbon source propionate (C) +0.1 +0.0 -0.2 +0.4 -1.2
carbon source L-Citrulline +0.3 +0.3 -0.6 -0.6 -0.0
carbon source Tween 20 (C) +0.0 +0.1 +0.1 +0.3 -1.1
nitrogen source Adenine (N) -0.1 -0.0 +0.1 -0.8 +0.4
nitrogen source nitrate (N) -0.1 +0.1 +0.2 -0.8 +0.2
carbon source acetate (C) -0.2 -0.3 +0.3 +0.5 -0.6
carbon source D-Glucose -0.0 +0.2 -0.2 -0.5 +0.5
stress Choline Lysine 12 mM +0.3 -0.0 -0.2 -0.2 +0.5
stress 1-ethyl-3-methylimidazolium acetate 30 mM +0.1 +0.1 -0.6 +0.2 +0.6
stress Cholin acetate 19 mM +0.1 -0.0 -0.5 +0.3 +0.5
denitrifying Nitrate reduction, replicate B +0.3 -0.1 -0.4 +0.4 +0.5
carbon source L-Citrulline +0.3 +0.4 -0.3 +0.0 +0.3
pH Minimal media pH9 +0.2 -0.1 -0.1 +0.4 +0.6
stress 1-ethyl-3-methylimidazolium lysine 10.5 mM +0.2 +0.1 -0.1 -0.2 +1.4
nitrogen source Parabanic Acid (N) +0.2 +0.2 +0.5 +0.3 +0.3
stress Benzalkonium Chloride 0.012 mg/ml +0.0 +0.1 -0.0 +0.3 +1.3
carbon source L-Citrulline -0.2 +0.4 +0.1 +0.9 +0.5
pH Minimal media pH9 +0.2 +0.3 +0.0 +0.8 +0.6
carbon source butyrate (C) +0.2 -2.3 +0.5 +0.8 +3.3
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