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Gene
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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_01175 and AO353_01180 are separated by 12 nucleotides
AO353_01180 and AO353_01185 are separated by 302 nucleotides
AO353_01185 and AO353_01190 are separated by 211 nucleotides
AO353_01190 and AO353_01195 are separated by 137 nucleotides
AO353_01175: AO353_01175 - hypothetical protein, at 260,151 to 261,101
_01175
AO353_01180: AO353_01180 - hypothetical protein, at 261,114 to 262,523
_01180
AO353_01185: AO353_01185 - superfamily II DNA/RNA helicase, SNF2 family protein, at 262,826 to 263,167
_01185
AO353_01190: AO353_01190 - hypothetical protein, at 263,379 to 263,615
_01190
AO353_01195: AO353_01195 - hypothetical protein, at 263,753 to 264,358
_01195
Group
Condition
AO353
_01175
AO353
_01180
AO353
_01185
AO353
_01190
AO353
_01195
pH
Minimal media pH9
-0.1
-0.1
+0.3
-4.4
-0.2
anaerobic
Anaerobic, acetate donor; nitrate and iron
-0.1
+0.5
-0.1
-3.0
-0.3
nitrogen source
L-Serine (N)
-0.1
+0.0
-0.5
-1.5
-0.0
stress
Doxycycline hyclate 0.0006 mg/ml
-0.4
-0.1
+0.3
-2.2
+0.5
motility
outer cut, LB soft agar motility assay
-0.0
+0.3
+0.4
-2.6
-0.0
pH
Minimal media pH9
-0.2
-0.1
+0.1
-1.1
-0.4
carbon source
L-Ornithine
-0.2
+0.3
+0.5
-2.2
-0.0
stress
Sisomicin 0.008 mg/ml
-0.2
+0.1
-0.1
-1.1
-0.3
stress
Rifampicin 0.005 mg/ml
+0.1
+0.0
-0.1
-1.3
-0.3
carbon source
Ethanol (C)
+0.2
+0.0
-0.1
-1.6
-0.1
nitrogen source
Gly-Glu (N)
-0.1
-0.0
-0.2
-1.3
+0.1
stress
Paraquat dichloride 0.032 mg/mL
-0.0
-0.0
+0.0
-1.6
+0.2
carbon source
succinate (C)
+0.2
-0.0
+0.2
-1.7
-0.0
nitrogen source
Thymine (N)
-0.3
+0.1
-0.1
-1.2
+0.2
carbon source
butyrate (C)
+0.0
+0.0
-0.3
-1.1
+0.2
nitrogen source
Gly-DL-Asp (N)
-0.1
+0.2
-0.1
-1.3
+0.4
stress
Doxycycline hyclate 0.0002 mg/ml
+0.1
-0.2
+0.3
-1.0
+0.0
carbon source
L-Tryptophan (C)
-0.0
+0.2
+0.2
-1.2
+0.0
carbon source
propionate (C)
+0.1
+0.1
+0.5
-1.4
+0.1
stress
Cobalt chloride 0.32 mM
+0.3
+0.1
+0.3
-0.9
-0.4
stress
Cisplatin 0.006 mg/ml
-0.3
-0.1
-0.3
+1.0
-0.3
stress
nitrate 200 mM
+0.2
+0.2
-0.5
+0.6
-0.1
stress
A22 0.002 mg/ml
+0.1
-0.1
-0.0
+0.9
-0.3
stress
1-ethyl-3-methylimidazolium acetate 30 mM
+0.1
+0.0
+0.3
+0.7
-0.4
carbon source
L-Citrulline
+0.1
+0.2
+0.6
+0.7
-0.4
carbon source
L-Ornithine
+0.0
-0.0
-0.2
+1.3
+0.2
carbon source
L-Citrulline
+0.1
+0.2
-0.2
+1.0
+0.2
pH
Minimal media pH7
-0.2
+0.1
+0.3
+1.3
-0.1
stress
Thallium(I) acetate 0.016 mg/ml
+0.3
+0.0
-0.0
+1.3
-0.1
temperature
LB 20C
+0.1
+0.0
+0.3
+1.2
+0.3
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AO353_01180
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AO353_01185
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AO353_01190
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