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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_01135 and AO353_01140 are separated by 214 nucleotides
AO353_01140 and AO353_01145 are separated by 142 nucleotides
AO353_01145 and AO353_01150 are separated by 108 nucleotides
AO353_01150 and AO353_01155 are separated by 107 nucleotides
AO353_01135: AO353_01135 - enoyl-CoA hydratase, at 250,811 to 251,500
_01135
AO353_01140: AO353_01140 - acyl-phosphate glycerol 3-phosphate acyltransferase, at 251,715 to 252,437
_01140
AO353_01145: AO353_01145 - magnesium transporter, at 252,580 to 253,551
_01145
AO353_01150: AO353_01150 - amidotransferase, at 253,660 to 254,388
_01150
AO353_01155: AO353_01155 - 3-hydroxyacyl-CoA dehydrogenase, at 254,496 to 256,640
_01155
Group
Condition
AO353
_01135
AO353
_01140
AO353
_01145
AO353
_01150
AO353
_01155
stress
Lomefloxacin 0.001 mM
-1.3
-0.5
-0.8
+0.5
-0.4
pH
Minimal media pH9
-2.0
-0.1
-0.0
-0.0
+0.1
nitrogen source
nitrite (N)
-1.1
+0.1
-0.5
-0.1
-0.1
nitrogen source
Parabanic Acid (N)
-0.6
-0.3
-0.4
-0.1
-0.1
stress
Fusidic 0.125 mg/ml
-0.9
-0.1
-0.2
-0.2
+0.0
nitrogen source
Gly-DL-Asp (N)
-0.5
-0.1
-0.5
-0.1
-0.1
anaerobic
Anaerobic, acetate donor; nitrate and iron
-2.5
+0.1
+0.1
+0.7
+0.3
temperature
LB 30C
-1.1
+0.1
-0.2
+0.2
-0.0
carbon source
L-Ornithine
-0.6
-0.1
+0.1
-0.3
-0.2
stress
Spectinomycin 0.2 mg/ml
-0.5
-0.2
-0.1
-0.3
+0.2
carbon source
L-Ornithine
+0.1
+0.0
-0.6
-0.6
+0.2
nitrogen source
L-Tryptophan (N)
-0.6
+0.2
+0.1
-0.2
-0.2
nitrogen source
Adenosine (N)
-0.4
+0.3
-0.3
+0.0
-0.3
stress
Thallium(I) acetate 0.008 mg/ml
+0.0
-0.3
-0.2
-0.5
+0.3
carbon source
L-Isoleucine (C)
-0.2
+0.4
-0.5
+0.0
-0.2
stress
Cisplatin 0.006 mg/ml
-0.7
+0.4
-0.6
+0.2
+0.2
carbon source
L-Citrulline
-0.5
+0.2
-0.4
-0.1
+0.3
nitrogen source
Glycine (N)
-0.8
+0.3
-0.0
+0.0
+0.1
carbon source
L-Leucine (C)
+0.3
+0.4
-0.6
-0.1
-0.3
carbon source
L-Ornithine
-0.5
+0.2
-0.3
+0.1
+0.1
carbon source
a-Ketoglutaric (C)
-0.7
+0.1
+0.3
-0.0
+0.3
carbon source
L-Citrulline
+0.3
-0.2
-0.4
+0.3
-0.1
stress
acetate 68 mM
+0.8
+0.1
-0.6
-0.2
-0.1
stress
Doxycycline hyclate 0.0006 mg/ml
-0.2
+0.7
-0.6
+0.1
+0.1
motility
outer cut, LB soft agar motility assay
-0.6
+0.5
-0.1
+0.2
+0.0
stress
Thallium(I) acetate 0.016 mg/ml
+0.5
-0.1
-0.4
-0.3
+0.4
stress
Benzalkonium Chloride 0.012 mg/ml
+0.9
-0.1
-0.3
+0.0
+0.1
carbon source
L-Aspartic Acid (C)
+0.8
-0.1
+0.3
-0.0
-0.1
survival
4C survival; 672 hrs
+0.4
+0.3
-0.1
+0.3
+0.2
stress
Nalidixic 0.004 mg/ml
+0.7
+0.2
+0.2
-0.2
+0.2
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