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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_00565 and AO353_00570 are separated by 124 nucleotides
AO353_00570 and AO353_00575 are separated by 101 nucleotides
AO353_00575 and AO353_00580 are separated by 204 nucleotides
AO353_00580 and AO353_00585 are separated by 98 nucleotides
AO353_00565: AO353_00565 - aconitate hydratase, at 118,133 to 120,883
_00565
AO353_00570: AO353_00570 - CAAX protease, at 121,008 to 121,799
_00570
AO353_00575: AO353_00575 - chemotaxis protein, at 121,901 to 123,466
_00575
AO353_00580: AO353_00580 - alpha/beta hydrolase, at 123,671 to 124,348
_00580
AO353_00585: AO353_00585 - cytochrome C oxidase Cbb3, at 124,447 to 125,871
_00585
Group
Condition
AO353
_00565
AO353
_00570
AO353
_00575
AO353
_00580
AO353
_00585
motility
outer cut, LB soft agar motility assay
+0.2
+0.1
-2.7
-0.4
-1.1
pH
Minimal media pH6
-0.0
-0.2
+0.2
-0.2
-1.9
pH
Minimal media pH8
+0.1
+0.0
+0.1
-0.3
-1.5
stress
acetate 68 mM
+0.1
-0.3
-0.6
-0.4
-0.5
survival
4C survival; 672 hrs
+0.0
-0.9
-0.1
-0.0
-0.4
motility
outer cut, LB soft agar motility assay
+0.3
-0.1
-1.1
-0.1
-0.4
survival
4C survival; 192 hrs
+0.2
+0.0
-0.1
-0.1
-1.4
stress
Choline chloride 358 mM
+0.1
-0.2
-0.2
-0.5
-0.3
nitrogen source
L-Glutamine (N)
-0.1
+0.1
+0.1
-0.3
-0.9
nitrogen source
L-Glutamic (N)
-0.0
+0.1
+0.1
-0.3
-0.8
nitrogen source
L-Histidine (N)
+0.0
-0.0
+0.1
-0.3
-0.8
nitrogen source
L-Arginine (N)
+0.1
-0.3
+0.2
+0.1
-1.0
nitrogen source
L-Arginine (N)
+0.1
-0.2
+0.1
-0.2
-0.7
stress
Cisplatin 0.006 mg/ml
-0.3
-0.3
+0.5
-0.4
-0.2
nitrogen source
L-Glutamine (N)
+0.1
+0.1
+0.2
-0.2
-1.1
motility
outer cut, LB soft agar motility assay
+0.4
+0.4
-1.0
-0.0
-0.5
stress
Nalidixic 0.004 mg/ml
+0.1
+0.2
-0.3
-0.4
-0.3
survival
4C survival; 360 hrs
+0.1
+0.1
+0.0
-0.1
-0.8
pH
Minimal media pH6
-0.0
+0.0
+0.4
-0.3
-0.7
carbon source
L-Histidine (C)
+0.0
+0.1
+0.2
+0.0
-1.0
stress
benzoic 0.58 mg/ml
-0.0
+0.4
-0.1
-0.4
-0.3
stress
Sisomicin 0.008 mg/ml
-0.0
-0.2
+0.1
-0.5
+0.3
stress
Cisplatin 0.006 mg/ml
-0.2
+0.4
+0.1
-0.3
-0.2
stress
Lomefloxacin 0.001 mM
+0.1
+0.5
+0.4
-0.5
-0.2
carbon source
L-Citrulline
+0.3
+0.5
+0.0
+0.2
-0.4
carbon source
L-Ornithine
+0.2
+0.4
+0.0
+0.3
-0.2
carbon source
L-Ornithine
+0.5
-0.1
+0.5
+0.2
-0.2
nitrogen source
L-tyrosine (N)
+0.1
+0.4
+0.2
+0.4
-0.1
carbon source
acetate (C)
+0.2
+0.7
-0.3
+0.0
+0.4
carbon source
L-Isoleucine (C)
+0.1
+0.5
+0.1
+0.3
+0.1
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