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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E3
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500 nt
AO353_00490 and AO353_00495 are separated by 143 nucleotides
AO353_00495 and AO353_00500 are separated by 114 nucleotides
AO353_00500 and AO353_00505 are separated by 100 nucleotides
AO353_00505 and AO353_00510 are separated by 30 nucleotides
AO353_00490: AO353_00490 - PasA protein, at 98,158 to 98,682
_00490
AO353_00495: AO353_00495 - DNA topoisomerase I, at 98,826 to 101,447
_00495
AO353_00500: AO353_00500 - TonB box-like protein, at 101,562 to 101,798
_00500
AO353_00505: AO353_00505 - 3-ketoacyl-CoA thiolase, at 101,899 to 103,074
_00505
AO353_00510: AO353_00510 - multifunctional fatty acid oxidation complex subunit alpha, at 103,105 to 105,252
_00510
Group
Condition
AO353
_00490
AO353
_00495
AO353
_00500
AO353
_00505
AO353
_00510
pH
Minimal media pH9
-1.2
-4.9
-0.5
-2.7
-3.0
carbon source
Tween 20 (C)
-0.7
-2.7
-0.4
-3.5
-3.5
carbon source
Tween 20 (C)
-1.0
-2.2
-0.5
-3.6
-3.4
carbon source
L-Tryptophan (C)
-1.1
-3.6
-0.6
-2.3
-2.6
pH
Minimal media pH9
-1.4
-3.3
-0.0
-2.4
-2.3
motility
outer cut, LB soft agar motility assay
-0.5
-3.6
-0.4
-1.6
-1.7
motility
outer cut, LB soft agar motility assay
-0.9
-3.1
-0.8
-0.5
-1.7
carbon source
L-Citrulline (C)
+0.1
-2.3
-0.6
-0.9
-2.6
motility
outer cut, LB soft agar motility assay
+0.1
-3.4
+0.1
-1.5
-1.4
carbon source
L-Leucine (C)
-1.0
+0.4
+0.3
-2.2
-3.2
carbon source
L-Valine (C)
-0.9
+0.3
-0.1
-2.7
-2.3
carbon source
L-Arginine (C)
-0.5
-1.8
+0.1
-1.3
-1.9
stress
Spectinomycin 0.15 mg/ml
-0.4
-2.5
+0.1
-0.9
-1.6
survival
4C survival; 360 hrs
-0.3
-3.0
+0.1
-0.7
-1.3
carbon source
L-Alanine (C)
-2.8
-2.4
+0.1
+0.3
-0.1
nitrogen source
Parabanic Acid (N)
-0.8
-2.9
-0.9
-0.4
+0.5
survival
4C survival; 672 hrs
-0.2
-2.9
+2.5
-1.8
-1.5
carbon source
Trisodium citrate (C)
-3.5
+1.5
-0.2
-0.6
-0.8
stress
Cisplatin 0.006 mg/ml
+1.3
-2.1
+0.2
-1.0
-1.4
carbon source
Trisodium citrate (C)
-2.9
+1.3
-0.3
-0.5
-0.5
stress
Doxycycline hyclate 0.0002 mg/ml
+0.5
-3.2
-0.8
+1.0
+0.9
stress
Sisomicin 0.008 mg/ml
+0.1
+4.8
-0.9
-2.4
-2.5
stress
Lomefloxacin 0.001 mM
-0.5
-2.6
-0.4
+1.1
+2.1
stress
Rifampicin 0.005 mg/ml
+0.2
-3.0
+0.5
+0.5
+1.8
stress
Tetracycline 0.0016 mg/ml
-0.6
-1.7
+0.5
+1.2
+2.0
stress
Doxycycline hyclate 0.0006 mg/ml
+0.9
-1.9
-0.2
+1.6
+2.0
carbon source
acetate (C)
-0.8
+4.1
+1.3
+0.5
+0.3
stress
Benzalkonium Chloride 0.01 mg/ml
+0.7
-0.0
+0.2
+2.7
+2.5
stress
Paraquat dichloride 0.032 mg/mL
+0.3
+5.6
+0.0
+0.7
+1.0
stress
Benzalkonium Chloride 0.012 mg/ml
+0.8
-0.4
+0.3
+4.0
+3.8
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