Fitness for 5 genes in Paraburkholderia graminis OAS925

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500 ntABIE53_003536 and ABIE53_003537 are separated by 108 nucleotidesABIE53_003537 and ABIE53_003538 are separated by 301 nucleotidesABIE53_003538 and ABIE53_003539 are separated by 115 nucleotidesABIE53_003539 and ABIE53_003540 are separated by 19 nucleotides ABIE53_003536: ABIE53_003536 - glyceraldehyde 3-phosphate dehydrogenase, at 3,974,802 to 3,975,812 _003536 ABIE53_003537: ABIE53_003537 - transketolase, at 3,975,921 to 3,977,942 _003537 ABIE53_003538: ABIE53_003538 - spermidine synthase, at 3,978,244 to 3,979,125 _003538 ABIE53_003539: ABIE53_003539 - putative glyoxalase superfamily protein PhnB, at 3,979,241 to 3,979,654 _003539 ABIE53_003540: ABIE53_003540 - 16S rRNA (uracil1498-N3)-methyltransferase, at 3,979,674 to 3,980,414 _003540
Group Condition ABIE53_003536 ABIE53_003537 ABIE53_003538 ABIE53_003539 ABIE53_003540
m9 no stress control M9 no stress control -5.4 N.D. -0.1 +0.0 -0.7
stress M9 with 3-methyl-3-butenol 4.5 g/L -5.8 N.D. -0.0 -0.1 -0.1
m9 no stress control M9 no stress control -5.0 N.D. -0.0 -0.2 -0.8
stress M9 with Isopropyl alcohol 21 g/L -5.9 N.D. -0.0 +0.0 -0.1
m9 no stress control M9 no stress control -5.2 N.D. -0.1 +0.0 -0.7
stress M9 with 3-methyl-3-butenol 4.5 g/L -5.7 N.D. -0.1 -0.1 +0.0
stress M9 with Isopropyl alcohol 21 g/L -5.8 N.D. +0.0 +0.1 +0.1
stress M9 with Propan-1-ol 13 g/L -5.7 N.D. +0.1 -0.0 +0.1
carbon source fumaric 20mM -4.9 N.D. -0.1 -0.1 -0.3
stress M9 with 3-methyl-3-butenol 4.5 g/L -5.3 N.D. +0.1 -0.1 -0.0
stress M9 with Isopropyl alcohol 21 g/L -5.2 N.D. -0.0 +0.0 -0.1
carbon source fumaric 20mM -4.6 N.D. -0.2 +0.0 -0.5
stress M9 with 3-methyl-3-butenol 4.5 g/L -5.0 N.D. +0.0 -0.1 -0.1
stress M9 with Propan-1-ol 13 g/L -5.2 N.D. +0.0 -0.0 +0.1
carbon source shik 5mM -4.8 N.D. -0.1 +0.0 -0.1
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 -4.9 N.D. -0.0 -0.1 +0.0
m9 no stress control M9 no stress control -4.1 N.D. -0.2 -0.2 -0.4
stress M9 with Ethanol 2.5 vol% -5.1 N.D. +0.0 +0.1 +0.2
stress M9 with Propan-1-ol 13 g/L -4.9 N.D. -0.0 +0.1 +0.0
stress M9 with Propan-1-ol 13 g/L -4.7 N.D. +0.1 -0.1 +0.2
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent -4.4 N.D. +0.1 -0.2 +0.3
carbon source shik 20mM -4.5 N.D. +0.1 +0.1 +0.2
in planta Plant=no_plant; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_liquid; Collection=outgrowth_R2A_with_Tween_and_Cycloheximide; Time=21_days -4.6 N.D. +0.0 +0.1 +1.1
in planta Plant=no_plant; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_liquid; Collection=outgrowth_R2A_with_Tween_and_Cycloheximide; Time=21_days -4.2 N.D. +0.2 +0.1 +0.6
carbon source 4-Hydroxybenzoic Acid (C) -3.9 N.D. -0.3 +0.0 +0.8
in planta Plant=no_plant; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_R2A_with_Tween_and_Cycloheximide; Time=21_days -4.2 N.D. +0.1 -0.0 +1.3
in planta Plant=no_plant; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_R2A_with_Tween_and_Cycloheximide; Time=21_days -4.0 N.D. +0.3 -0.1 +1.0
in planta Plant=no_plant; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_R2A_with_Tween_and_Cycloheximide; Time=21_days -3.4 N.D. +0.1 -0.2 +1.3
carbon source Acetylated xylan 10 g/L (C) -2.9 N.D. +0.2 +0.2 +1.9
plant outgrowth control Plant outgrowth control -0.9 N.D. +0.5 +0.5 +3.2
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