Fitness for 5 genes in Bacteroides thetaiotaomicron VPI-5482

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500 ntBT4714 and BT4715 are separated by 105 nucleotidesBT4715 and BT4716 are separated by 136 nucleotidesBT4716 and BT4717 are separated by 6 nucleotidesBT4717 and BT4718 are separated by 142 nucleotides BT4714: BT4714 - periplasmic beta-glucosidase precursor, xylosidase/arabinosidase (NCBI ptt file), at 6,188,192 to 6,190,501 BT4714 BT4715: BT4715 - non-specific DNA-binding protein Dps (NCBI ptt file), at 6,190,607 to 6,191,083 BT4715 BT4716: BT4716 - redox-sensitive transcriptional activator (NCBI ptt file), at 6,191,220 to 6,192,146 BT4716 BT4717: BT4717 - integral membrane protein, putative permease (NCBI ptt file), at 6,192,153 to 6,193,085 BT4717 BT4718: BT4718 - putative aquaporin (NCBI ptt file), at 6,193,228 to 6,193,896 BT4718
Group Condition BT4714 BT4715 BT4716 BT4717 BT4718
carbon source Heparin from porcine intestinal mucosa (C) +0.2 -0.1 -1.0 -0.7 -0.9
carbon source Mannan from Saccharomyces cerevisiae; filtered 2.5 mg/ml (C) -0.4 -0.6 -0.3 -0.4 -0.5
carbon source Mannan from Saccharomyces cerevisiae (C) -0.5 -0.6 -0.6 -0.9 +0.4
carbon source Mannan from Saccharomyces cerevisiae; autoclaved 2.5 mg/ml (C) -0.3 -0.9 -0.7 -0.2 +0.1
survival aerobic shaking 48 hr copy B +0.1 -0.4 -1.4 +0.1 -0.3
mouse_peg_treatment PEG (Day 1) - Cage4;Mouse1 +0.0 -0.9 -0.3 -0.6 -0.1
phage DAC16_cps7_10MOI +0.1 -1.4 -0.4 +0.1 -0.1
phage SJC25_Acapsular_0.1MOI +0.4 -1.0 -0.7 -0.2 -0.2
mouse_post_colonization Post-colonization (Day 2) - Cage3;Mouse2 +0.1 -0.2 -1.2 +0.1 -0.4
stress BHIS with Paraquat dichloride 0.1 mg/ml +0.3 -0.2 -0.7 -0.0 -0.8
mouse_peg_treatment PEG (Day 3) - Cage1;Mouse2 +0.1 -0.7 -0.7 +0.3 -0.5
stress BHIS with Thioridazine 0.0125 mM +0.1 -0.2 -0.7 +0.3 -0.8
phage SJC16_cps8_1MOI +0.6 -0.4 -1.0 +0.1 -0.6
phage HNL05_cps7_0.1MOI +0.0 -0.8 -0.6 +0.4 -0.3
phage ARB14_cps1_0.1MOI +0.2 -0.2 -0.8 +0.4 -0.5
stress Varel_Bryant_medium_Glucose with Trimethoprim 0.025 mM +0.4 -1.2 -0.1 +0.4 -0.3
nitrogen source L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen +0.5 -0.3 -0.7 +0.3 -0.6
stress BHIS with Sulfaquinoxaline 0.1 mM +0.4 -0.9 -0.4 -0.1 +0.3
phage SJC16_cps8_10MOI +0.5 -0.1 -0.8 +0.3 -0.4
phage SJC12_cps8_0.1MOI +0.4 -0.3 -0.3 +0.4 -0.7
mouse_coculture cage1; mouse1; day3; diet=StandardDiet; sample=FecalPellet; coculture=Bbreve_UCC2003 +0.3 +0.4 -0.9 +0.2 -0.3
stress BHIS with Sulfamonomethoxine 0.2 mM +0.5 +0.2 -0.6 +0.3 -0.5
mouse_coculture cage1; mouse3; day4; diet=StandardDiet; sample=FecalPellet; coculture=Bbreve_UCC2003 +0.3 +0.5 -0.7 +0.2 -0.3
stress BHIS with Chlorpromazine 0.2 mM +0.3 -0.0 -0.4 +0.8 -0.5
stress Varel_Bryant_medium_Glucose with Ciprofloxacin Hydrochloride 0.025 mM +0.5 -0.7 -0.1 +0.4 +0.4
nitrogen source L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen +0.2 -0.0 -0.7 +1.1 +0.0
survival aerobic shaking 48 hr copy A +0.4 +0.2 -0.8 +0.7 +0.2
stress BHIS with Simvastatin 30 uM +0.3 +0.6 -0.5 -0.3 +0.7
stress Varel_Bryant_medium_Glucose with Bile salts 0.5 mg/mL; pH 7 +0.2 +1.0 -0.1 +0.4 -0.3
stress BHIS with Lithocholic 0.4 mg/ml +0.3 +0.7 +0.4 +0.6 +0.1
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