Fitness for 5 genes in Bacteroides thetaiotaomicron VPI-5482

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500 ntBT3019 and BT3020 are separated by 81 nucleotidesBT3020 and BT3021 are separated by 144 nucleotidesBT3021 and BT3022 are separated by 74 nucleotidesBT3022 and BT3023 are separated by 388 nucleotides BT3019: BT3019 - putative Na+/Ca2+ exchanger protein, cation antiporter (NCBI ptt file), at 3,818,218 to 3,819,204 BT3019 BT3020: BT3020 - conserved hypothetical protein (NCBI ptt file), at 3,819,286 to 3,820,827 BT3020 BT3021: BT3021 - conserved hypothetical protein (NCBI ptt file), at 3,820,972 to 3,822,324 BT3021 BT3022: BT3022 - hypothetical protein (NCBI ptt file), at 3,822,399 to 3,822,590 BT3022 BT3023: BT3023 - conserved hypothetical protein (NCBI ptt file), at 3,822,979 to 3,823,896 BT3023
Group Condition BT3019 BT3020 BT3021 BT3022 BT3023
carbon source Beta-Lactose 2.5 mg/ml (C) +0.1 +0.0 +0.0 -3.9 +0.0
stress BHIS with Simvastatin 30 uM -0.2 -0.5 -0.1 -3.0 +0.0
stress 0.35mg/ml WT Calprotectin + 1uM saturated Fe-Enterobactin in 50% 50%BHI + 50% Calprotectin buffer + 1x protoporphyrin, cultured for 20 hours. -0.2 -0.1 +0.0 -3.0 -0.3
mouse_post_colonization Post-colonization (Day 10) - Cage5;Mouse3 -0.1 -0.2 +0.2 -3.5 +0.4
stress BHIS with Oxytetracycline 0.000390625 mM -0.4 -0.2 -0.1 -2.1 -0.2
carbon source D-Trehalose 20mM (C) +0.2 -0.2 +0.1 -3.1 +0.1
nitrogen source L-Glutamine (N); Varel_Bryant_medium_Glucose_DTT_NaS_noNitrogen -0.3 -0.0 +0.2 -2.7 -0.0
carbon source (+)-Arabinogalactan (C) +0.1 +0.2 -0.0 -2.7 -0.5
mouse_post_colonization Post-colonization (Day 12) - Cage1;Mouse5 -0.2 +0.0 +0.0 -2.7 +0.0
mouse_post_colonization Post-colonization (Day 12) - Cage5;Mouse2 +0.4 +0.1 +0.1 -3.4 +0.1
carbon source Mannan from Saccharomyces cerevisiae (C) -0.0 +0.2 -0.0 -2.0 -0.8
stress Varel_Bryant_medium_Glucose with Geneticin G418 0.2 mM -0.3 +0.2 +0.0 -2.3 -0.0
stress BHIS with Dimethyl Sulfoxide 0.2 vol% -0.0 +0.1 +0.0 -2.5 +0.3
carbon source Red Arabinan from sugar-beet -0.1 +0.3 +0.1 -1.9 -0.4
stress BHIS with Dimethyl Sulfoxide 0.2 vol% -0.1 +0.1 +0.2 -2.1 +0.2
mouse_post_colonization Post-colonization (Day 12) - Cage1;Mouse3 +0.2 +0.1 +0.2 -2.3 +0.3
stress BHIS with Antimycin A 0.1 mM +0.0 +0.0 +0.2 -2.1 +0.3
stress BHIS with Doxycycline hyclate 0.000390625 mM +0.4 +0.3 +0.4 -2.5 -0.0
mouse_post_colonization Post-colonization (Day 12) - Cage3;Mouse4 +0.3 +0.0 +0.3 -1.9 +0.2
stress Varel_Bryant_medium_Glucose with Chlorpromazine 0.1 mM -0.6 -0.3 -0.2 +2.1 +0.0
stress Varel_Bryant_medium_Glucose with Doxycycline hyclate 0.0001953125 mM -0.5 +0.1 +0.1 +1.8 -0.3
phage SJC15_cps8_1MOI -0.3 -0.2 +0.2 +1.8 +0.4
stress Varel_Bryant_medium_Glucose with Oxytetracycline 0.000390625 mM -0.4 +0.1 +0.0 +2.3 -0.1
stress Varel_Bryant medium with Chlorhexidine diacetate salt 0.0015 mM -0.2 -0.2 +0.1 +2.0 +0.1
mouse_peg_treatment PEG (Day 3) - Cage2;Mouse1 -0.5 +0.3 -0.0 +2.0 +0.2
stress Varel_Bryant_medium_Glucose with Lincomycin 0.0015625 mM -0.1 +0.2 +0.2 +2.1 -0.1
stress Varel_Bryant_medium_Glucose with Chenodeoxycholic 0.5 mg/ml +0.1 +0.1 +0.0 +2.3 +0.1
stress Varel_Bryant_medium_Glucose with Sulfaquinoxaline 0.1 mM -0.2 +0.1 +0.1 +2.6 +0.0
phage ARB14_cps1_0.1MOI +0.2 -0.3 -0.0 +2.5 +0.6
stress Varel_Bryant_medium_Glucose with Phenazine ethosulfate 0.1 mM -0.2 +0.0 +0.2 +2.7 +0.2
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