Fitness for 5 genes in Bacteroides thetaiotaomicron VPI-5482

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500 ntBT0129 and BT0130 are separated by 12 nucleotidesBT0130 and BT0131 are separated by 381 nucleotidesBT0131 and BT0132 are separated by 239 nucleotidesBT0132 and BT0133 overlap by 17 nucleotides BT0129: BT0129 - mannonate dehydratase (NCBI ptt file), at 124,349 to 125,536 BT0129 BT0130: BT0130 - putative oxidoreductase (NCBI ptt file), at 125,549 to 126,364 BT0130 BT0131: BT0131 - putative transposase (NCBI ptt file), at 126,746 to 127,678 BT0131 BT0132: BT0132 - alpha-glucosidase, putative (NCBI ptt file), at 127,918 to 129,852 BT0132 BT0133: BT0133 - putative sugar phosphate isomerase (NCBI ptt file), at 129,836 to 130,669 BT0133
Group Condition BT0129 BT0130 BT0131 BT0132 BT0133
stress BHIS with Dimethyl Sulfoxide 10 vol% +0.4 -3.0 N.D. -0.3 -0.3
stress BHIS with Dimethyl Sulfoxide 10 vol% -0.0 -2.1 N.D. -0.7 +0.0
stress BHIS with Antimycin A 0.1 mM -0.1 -1.4 N.D. -0.5 -0.6
stress BHIS with Dimetridazole 0.00625 mM +0.1 -1.6 N.D. +0.0 -1.0
stress Varel_Bryant medium with Irgasan 0.0025 mM +0.1 -1.3 N.D. -0.6 -0.4
carbon source B-glucan from yeast; autoclaved 2.5 mg/ml (C) -0.4 -1.3 N.D. +0.1 -0.6
stress Varel_Bryant_medium_Glucose with Zinc Pyrithione 0.05 mM -0.3 -0.4 N.D. +0.1 -1.6
stress BHIS with Cephalothin 0.1 mM -0.5 -1.7 N.D. -0.0 +0.1
stress BHIS with Oxytetracycline 0.0001953125 mM -0.2 -2.5 N.D. +0.2 +0.5
stress BHIS with Dimethyl Sulfoxide 9 vol% +0.0 -1.5 N.D. -0.8 +0.4
stress BHIS with Doxycycline hyclate 0.0001953125 mM +0.4 -1.4 N.D. -0.5 -0.3
stress BHIS with Chloride 350 mM +0.5 -1.7 N.D. -0.5 -0.0
stress BHIS with Fusidic 0.002 mg/ml +0.2 -1.8 N.D. -0.2 +0.2
stress BHIS with Doxycycline hyclate 0.00009765625 mM +0.3 -1.4 N.D. +0.3 -0.5
stress BHIS with Cephalothin 0.625 mg/ml -0.3 -1.5 N.D. +0.5 +0.2
carbon source Mannan from Saccharomyces cerevisiae (C) +0.4 +0.6 N.D. -0.3 -1.3
carbon source Mannan from Saccharomyces cerevisiae (C) +0.6 +0.6 N.D. -1.1 -0.5
carbon source Mannan from Saccharomyces cerevisiae; filtered 2.5 mg/ml (C) +0.4 +0.7 N.D. -0.6 -0.8
nitrogen source L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen +0.5 +0.9 N.D. +0.5 -1.3
stress BHIS with Trimethoprim 0.0125 mM -0.8 +0.5 N.D. +0.6 +0.5
stress Varel_Bryant_medium_Glucose with Bile salts 0.5 mg/mL; pH 7 +0.5 +1.2 N.D. -0.4 -0.3
stress Varel_Bryant_medium_Glucose with Chenodeoxycholic 0.25 mg/ml +0.6 +1.1 N.D. -0.4 -0.3
stress BHIS with Doxycycline hyclate 0.0001953125 mM -0.3 -0.4 N.D. +0.9 +0.8
stress Varel_Bryant_medium_Glucose with Sulfaquinoxaline 0.2 mM -0.1 +1.8 N.D. +0.1 -0.4
stress BHIS with Chlorpromazine 0.1 mM -0.4 +0.7 N.D. +0.6 +0.7
stress BHIS with Thioridazine 0.025 mM -0.3 +0.7 N.D. +0.3 +1.1
survival aerobic shaking 48 hr copy A -0.3 +0.5 N.D. +0.6 +1.0
stress BHIS with 5-Fluorouracil 0.2 mM -0.4 +1.5 N.D. +0.3 +0.6
stress BHIS with Zinc Pyrithione 0.05 mM -0.0 +1.7 N.D. +0.1 +0.6
stress BHIS with Thioridazine 0.0125 mM -0.6 +2.2 N.D. +0.3 +1.0
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