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Fitness for 5 genes in
Shewanella oneidensis MR-1
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500 nt
metA and atoB are separated by 430 nucleotides
atoB and mmsA are separated by 136 nucleotides
mmsA and SO1679 are separated by 162 nucleotides
SO1679 and SO1680 are separated by 52 nucleotides
SO1676: metA - homoserine O-succinyltransferase (NCBI ptt file), at 1,761,811 to 1,762,752
metA
SO1677: atoB - 3-ketoacyl-CoA thiolase [isoleucine degradation] (EC 2.3.1.16)
(from data)
, at 1,763,183 to 1,764,373
atoB
SO1678: mmsA - methylmalonate-semialdehyde dehydrogenase (NCBI ptt file), at 1,764,510 to 1,766,009
mmsA
SO1679: SO1679 - 2-methylbutanoyl-CoA dehydrogenase (EC 1.3.8.5)
(from data)
, at 1,766,172 to 1,767,329
SO1679
SO1680: SO1680 - enoyl-CoA hydratase/isomerase family protein (NCBI ptt file), at 1,767,382 to 1,768,155
SO1680
Group
Condition
SO1676
SO1677
SO1678
SO1679
SO1680
metA
atoB
mmsA
carbon source
L-Isoleucine (C)
-6.0
-5.1
-5.5
-5.5
-5.0
carbon source
L-Isoleucine (C)
-5.8
-4.6
-5.0
-5.0
-4.3
nitrogen source
L-Isoleucine (N)
-6.9
-1.2
-2.0
-1.6
-0.8
nitrogen source
L-Isoleucine (N)
-5.7
-1.6
-1.7
-2.1
-1.1
nitrogen source
L-Phenylalanine (N)
-7.6
+0.4
-0.3
-0.1
-2.6
carbon source
NAG (C)
-6.7
-1.2
-0.2
-0.5
-1.0
temperature
Minimal 30C
-7.5
-0.6
-0.3
-0.6
-0.5
nitrogen source
L-Phenylalanine (N)
-7.4
+0.5
-0.1
+0.3
-2.9
pH
ShewMM_MES_buffer
-7.6
-0.4
+0.1
-0.6
-0.4
nitrogen source
L-Serine (N)
-7.8
-0.2
-0.9
-0.1
+0.3
nitrogen source
L-Asparagine (N)
-7.6
+0.1
-0.3
-0.1
-0.6
carbon source
pyruvate (C)
-7.7
-0.3
-0.1
+0.1
-0.4
carbon source
Cytidine (C)
-7.9
-0.4
+0.4
-0.0
-0.4
carbon source
propionate (C)
-6.3
-0.5
-0.6
-0.6
-0.4
nitrogen source
L-Arginine (N)
-7.9
-0.2
-0.3
+0.3
-0.2
nitrogen source
L-Threonine (N)
-8.7
+0.2
-0.1
+0.2
+0.2
pH
ShewMM
-7.7
-0.3
+0.4
-0.1
-0.4
nitrogen source
L-Threonine (N)
-8.4
+0.3
+0.0
+0.1
+0.4
nitrogen source
L-Serine (N)
-7.5
-0.3
-0.0
-0.0
+0.4
nitrogen source
Putrescine (N)
-7.2
+0.1
+0.0
+0.3
-0.6
nitrogen source
Ammonium chloride (N)
-7.8
+0.0
+0.1
+0.4
+0.1
nitrogen source
NAG (N)
-7.3
+0.5
+0.3
+0.5
-0.5
nitrogen source
Ammonium chloride (N)
-7.2
+0.6
+0.4
+0.5
-0.2
nitrogen source
NAG (N)
-7.4
+0.4
+1.1
+0.6
-0.6
nitrogen source
L-Alanine (N)
-7.5
+0.6
+1.1
+1.1
-0.6
nitrogen source
Ammonium chloride (N)
-6.8
+0.8
+0.4
+0.6
-0.0
carbon source
D,L-Lactate (C)
-6.5
+0.4
+0.3
+1.1
-0.1
nitrogen source
L-Alanine (N)
-6.2
+0.7
+0.8
+0.4
-0.5
carbon source
L-Lactate (C)
-6.5
+1.5
+1.9
+2.0
+0.1
carbon source
L-Lactate (C)
-6.5
+2.1
+2.4
+2.8
+0.2
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