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Fitness for 5 genes in
Shewanella oneidensis MR-1
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500 nt
SO0208.1 and SO0211 are separated by 22 nucleotides
SO0211 and murB are separated by 277 nucleotides
murB and birA overlap by 8 nucleotides
birA and panK are separated by 2 nucleotides
SO0208.1: SO0208.1 - putative transposase (NCBI ptt file), at 222,590 to 222,898
SO0208.1
SO0211: SO0211 - ISSod10, transposase OrfA (NCBI ptt file), at 222,921 to 223,361
SO0211
SO0213: murB - UDP-N-acetylenolpyruvoylglucosamine reductase (NCBI ptt file), at 223,639 to 224,664
murB
SO0214: birA - birA bifunctional protein (NCBI ptt file), at 224,657 to 225,616
birA
SO0215: panK - pantothenate kinase (NCBI ptt file), at 225,619 to 226,569
panK
Group
Condition
SO0208.1
SO0211
SO0213
SO0214
SO0215
murB
birA
panK
stress
Carbenicillin 0.008 mg/ml
-4.8
+0.4
N.D.
N.D.
N.D.
micoeukaryotes
C.elegans, mixed culture-2
-4.2
+0.0
N.D.
N.D.
N.D.
evt
fumarate electron acceptor
-4.0
+0.1
N.D.
N.D.
N.D.
evt
LB; anaerobic
-3.7
+0.4
N.D.
N.D.
N.D.
survival
Survival in PBS at 22C, experiment2, 13 day
-3.5
+0.3
N.D.
N.D.
N.D.
flight_in_space
LB; anaerobic; flight
-2.2
+0.0
N.D.
N.D.
N.D.
rnaprotect test
LB - with RNA protect
-2.0
-0.3
N.D.
N.D.
N.D.
flight_in_space
fumarate electron acceptor; flight
-2.3
+0.5
N.D.
N.D.
N.D.
evt
fumarate electron acceptor
-2.0
+0.2
N.D.
N.D.
N.D.
carbon source
Inosine (C)
-1.5
-0.2
N.D.
N.D.
N.D.
ground_control
Fumarate electron acceptor; ground control
-1.5
-0.0
N.D.
N.D.
N.D.
survival
Survival in PBS at 22C, experiment1, 6 day
-1.5
-0.0
N.D.
N.D.
N.D.
survival
Survival in PBS at 22C, experiment2, 6 day
-1.4
-0.0
N.D.
N.D.
N.D.
motility
M1 center
-1.8
+0.3
N.D.
N.D.
N.D.
nitrogen source
L-Methionine (N)
-0.9
-0.5
N.D.
N.D.
N.D.
ground_control
fumarate electron acceptor
-1.1
-0.3
N.D.
N.D.
N.D.
stress
copper (II) chloride 0.32 mM
-1.2
-0.2
N.D.
N.D.
N.D.
evt
Ferric citrate electron acceptor
-1.3
-0.1
N.D.
N.D.
N.D.
survival
Survival in PBS at 22C, experiment1, 13 day
-1.2
-0.1
N.D.
N.D.
N.D.
stress
Nickel (II) chloride 0.4 mM
-0.8
-0.5
N.D.
N.D.
N.D.
stress
Chlorite 0.64 mM
-1.0
-0.3
N.D.
N.D.
N.D.
carbon source
L-Glutamine (C)
-1.1
-0.2
N.D.
N.D.
N.D.
pH
ShewMM
-1.2
+0.3
N.D.
N.D.
N.D.
stress
Cobalt chloride 0.055 mM
-1.1
+0.4
N.D.
N.D.
N.D.
evt
fumarate electron acceptor
+1.1
-0.2
N.D.
N.D.
N.D.
nitrogen source
L-Serine (N)
+1.5
-0.2
N.D.
N.D.
N.D.
nitrogen source
L-Serine (N)
+1.4
+0.2
N.D.
N.D.
N.D.
ground_control
LB anaerobic; ground control
+1.6
+0.3
N.D.
N.D.
N.D.
ground_control
Fumarate electron acceptor; ground control
+2.1
-0.1
N.D.
N.D.
N.D.
motility
M5 outer
+2.0
+0.1
N.D.
N.D.
N.D.
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