Gene info for Pf6N2E2_480 in Pseudomonas fluorescens FW300-N2E2

Updated annotation (from data): 3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) (EC 1.2.4.4)
Rationale: Specifically important for utilizing L-Isoleucine. Automated validation from mutant phenotype: the predicted function (1.2.4.4) was linked to the condition via a SEED subsystem. This annotation was also checked manually.
Original annotation: Branched-chain alpha-keto acid dehydrogenase, E1 component, beta subunit (EC 1.2.4.4)
Type 1: Protein-coding gene
Located on scaffold unitig_0|quiver.unitig_1|quiver|pilon, - strand, nucleotides 546,161 to 547,219
See nucleotide sequence around Pf6N2E2_480 or for all of scaffold unitig_0|quiver.unitig_1|quiver|pilon

Other databases: refseq: TK06_RS02400, uniprot: A0A159ZTL1

Nearby Genes

500 ntPf6N2E2_475 and Pf6N2E2_476 are separated by 110 nucleotidesPf6N2E2_476 and Pf6N2E2_477 are separated by 175 nucleotidesPf6N2E2_477 and Pf6N2E2_478 are separated by 283 nucleotidesPf6N2E2_478 and Pf6N2E2_479 are separated by 3 nucleotidesPf6N2E2_479 and Pf6N2E2_480 are separated by 0 nucleotidesPf6N2E2_480 and Pf6N2E2_481 are separated by 1 nucleotidesPf6N2E2_481 and Pf6N2E2_482 are separated by 192 nucleotidesPf6N2E2_482 and Pf6N2E2_483 are separated by 151 nucleotidesPf6N2E2_483 and Pf6N2E2_484 are separated by 320 nucleotidesPf6N2E2_484 and Pf6N2E2_485 are separated by 196 nucleotides Pf6N2E2_475 - Transcriptional regulator, AraC family, at 540,187 to 540,981 _475 Pf6N2E2_476 - Permease of the drug/metabolite transporter (DMT) superfamily, at 541,092 to 542,021 _476 Pf6N2E2_477 - Branched-chain amino acid aminotransferase (EC 2.6.1.42), at 542,197 to 543,216 _477 Pf6N2E2_478 - Dihydrolipoyl dehydrogenase (EC 1.8.1.4) (from data), at 543,500 to 544,885 _478 Pf6N2E2_479 - Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase (EC 2.3.1.168) (from data), at 544,889 to 546,160 _479 Pf6N2E2_480 - 3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) (EC 1.2.4.4) (from data), at 546,161 to 547,219 _480 Pf6N2E2_481 - branched-chain alpha-ketoacid dehydrogenase, E1 component alpha subunit (EC 1.2.4.4) (from data), at 547,221 to 548,456 _481 Pf6N2E2_482 - Transcriptional regulator BkdR of isoleucine and valine catabolism operon, at 548,649 to 549,137 _482 Pf6N2E2_483 - FIG146518: Zn-dependent hydrolases, including glyoxylases, at 549,289 to 550,152 _483 Pf6N2E2_484 - Aldose 1-epimerase, at 550,473 to 551,342 _484 Pf6N2E2_485 - TRAP-type C4-dicarboxylate transport system, large permease component, at 551,539 to 552,819 _485
Locus Name Description Strand Phenotype
Pf6N2E2_475 Transcriptional regulator, AraC family - insig.
Pf6N2E2_476 Permease of the drug/metabolite transporter (DMT) superfamily + sig.
Pf6N2E2_477 Branched-chain amino acid aminotransferase (EC 2.6.1.42) - strong
Pf6N2E2_478 Dihydrolipoyl dehydrogenase (EC 1.8.1.4) (from data) - strong
Pf6N2E2_479 Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase (EC 2.3.1.168) (from data) - strong
Pf6N2E2_480 3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) (EC 1.2.4.4) (from data) - strong
Pf6N2E2_481 branched-chain alpha-ketoacid dehydrogenase, E1 component alpha subunit (EC 1.2.4.4) (from data) - strong
Pf6N2E2_482 Transcriptional regulator BkdR of isoleucine and valine catabolism operon + strong
Pf6N2E2_483 FIG146518: Zn-dependent hydrolases, including glyoxylases - insig.
Pf6N2E2_484 Aldose 1-epimerase - insig.
Pf6N2E2_485 TRAP-type C4-dicarboxylate transport system, large permease component - insig.

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