Gene info for Pf6N2E2_4704 in Pseudomonas fluorescens FW300-N2E2

Updated annotation (from data): Dimethylglycine dehydrogenase (ferredoxin), FeS subunit DgcB (EC 1.5.7.-)
Rationale: Specific phenotype: utilization of Carnitine. DgcB is quite similar to csal_0991, which is the putative FeS component of a N,N-dimethylglycine/sarcosine dehydrogenase that forms formaldehyde and glycine (PMC2293255). However the Pseudomonas enzyme probably acts primarily on dimethylglycine in vivo, as another sarcosine-specific system is important for carnitine utilization (Pf6N2E2_4720).
Original annotation: Predicted L-lactate dehydrogenase, Iron-sulfur cluster-binding subunit YkgF
Type 1: Protein-coding gene
Located on scaffold unitig_0|quiver.unitig_1|quiver|pilon, + strand, nucleotides 5,436,140 to 5,438,089
See nucleotide sequence around Pf6N2E2_4704 or for all of scaffold unitig_0|quiver.unitig_1|quiver|pilon

Other databases: refseq: TK06_RS23510, uniprot: A0A160A207

Nearby Genes

500 ntPf6N2E2_4699 and Pf6N2E2_4700 are separated by 234 nucleotidesPf6N2E2_4700 and Pf6N2E2_4701 are separated by 80 nucleotidesPf6N2E2_4701 and Pf6N2E2_4702 are separated by 15 nucleotidesPf6N2E2_4702 and Pf6N2E2_4703 are separated by 66 nucleotidesPf6N2E2_4703 and Pf6N2E2_4704 are separated by 27 nucleotidesPf6N2E2_4704 and Pf6N2E2_4705 overlap by 1 nucleotidesPf6N2E2_4705 and Pf6N2E2_4706 are separated by 13 nucleotidesPf6N2E2_4706 and Pf6N2E2_4707 are separated by 250 nucleotidesPf6N2E2_4707 and Pf6N2E2_4708 are separated by 888 nucleotidesPf6N2E2_4708 and Pf6N2E2_4709 overlap by 42 nucleotides Pf6N2E2_4699 - FIG00953563: hypothetical protein, at 5,431,632 to 5,432,033 _4699 Pf6N2E2_4700 - Microsomal dipeptidase (EC 3.4.13.19), at 5,432,268 to 5,433,245 _4700 Pf6N2E2_4701 - FIG00953113: hypothetical protein, at 5,433,326 to 5,433,856 _4701 Pf6N2E2_4702 - DgcA Dimethylglycine demethylase subunit A, at 5,433,872 to 5,435,932 _4702 Pf6N2E2_4703 - hypothetical protein, at 5,435,999 to 5,436,112 _4703 Pf6N2E2_4704 - Dimethylglycine dehydrogenase (ferredoxin), FeS subunit DgcB (EC 1.5.7.-) (from data), at 5,436,140 to 5,438,089 _4704 Pf6N2E2_4705 - Electron transfer flavoprotein, alpha subunit, at 5,438,089 to 5,439,309 _4705 Pf6N2E2_4706 - Electron transfer flavoprotein, beta subunit, at 5,439,323 to 5,440,093 _4706 Pf6N2E2_4707 - acetyltransferase, GNAT family, at 5,440,344 to 5,440,910 _4707 Pf6N2E2_4708 - hypothetical protein, at 5,441,799 to 5,442,002 _4708 Pf6N2E2_4709 - Cholesterol oxidase (EC 1.1.3.6), at 5,441,961 to 5,445,413 _4709
Locus Name Description Strand Phenotype
Pf6N2E2_4699 FIG00953563: hypothetical protein - sig.
Pf6N2E2_4700 Microsomal dipeptidase (EC 3.4.13.19) + strong
Pf6N2E2_4701 FIG00953113: hypothetical protein + strong
Pf6N2E2_4702 DgcA Dimethylglycine demethylase subunit A + strong
Pf6N2E2_4703 hypothetical protein - insig.
Pf6N2E2_4704 Dimethylglycine dehydrogenase (ferredoxin), FeS subunit DgcB (EC 1.5.7.-) (from data) + strong
Pf6N2E2_4705 Electron transfer flavoprotein, alpha subunit + strong
Pf6N2E2_4706 Electron transfer flavoprotein, beta subunit + sig.
Pf6N2E2_4707 acetyltransferase, GNAT family + insig.
Pf6N2E2_4708 hypothetical protein - insig.
Pf6N2E2_4709 Cholesterol oxidase (EC 1.1.3.6) + insig.

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