Gene info for Pf6N2E2_2047 in Pseudomonas fluorescens FW300-N2E2

Updated annotation (from data): D-glucosaminate dehydratase (EC 4.3.1.9)
Rationale: Specifically important for: D-Glucosamine Hydrochloride. This enzyme had not previously been linked to a gene. This is the second step in catabolism of glucosamine, and the 'beta' form of the enzyme was expected to be PLP-dependent and about this size. Iwamoto et al (2003) purified a non-specific 'alpha' enzyme for this reaction (PMID: 12686150)
Original annotation: D-serine deaminase (EC 4.3.1.18)
Type 1: Protein-coding gene
Located on scaffold unitig_0|quiver.unitig_1|quiver|pilon, - strand, nucleotides 2,438,916 to 2,440,133
See nucleotide sequence around Pf6N2E2_2047 or for all of scaffold unitig_0|quiver.unitig_1|quiver|pilon

Other databases: refseq: TK06_RS10360, uniprot: A0A159ZWW4

Nearby Genes

500 ntPf6N2E2_2042 and Pf6N2E2_2043 are separated by 43 nucleotidesPf6N2E2_2043 and Pf6N2E2_2044 are separated by 60 nucleotidesPf6N2E2_2044 and Pf6N2E2_2045 are separated by 48 nucleotidesPf6N2E2_2045 and Pf6N2E2_2046 are separated by 198 nucleotidesPf6N2E2_2046 and Pf6N2E2_2047 are separated by 138 nucleotidesPf6N2E2_2047 and Pf6N2E2_2048 are separated by 41 nucleotidesPf6N2E2_2048 and Pf6N2E2_2049 are separated by 234 nucleotidesPf6N2E2_2049 and Pf6N2E2_2050 are separated by 25 nucleotidesPf6N2E2_2050 and Pf6N2E2_2051 overlap by 4 nucleotidesPf6N2E2_2051 and Pf6N2E2_2052 are separated by 9 nucleotides Pf6N2E2_2042 - Acetoacetyl-CoA synthetase (EC 6.2.1.16), at 2,431,553 to 2,433,508 _2042 Pf6N2E2_2043 - Twitching motility protein PilT, at 2,433,552 to 2,434,739 _2043 Pf6N2E2_2044 - Histidine kinase/response regulator hybrid protein, at 2,434,800 to 2,437,337 _2044 Pf6N2E2_2045 - Peptidyl-prolyl cis-trans isomerase PpiC (EC 5.2.1.8), at 2,437,386 to 2,437,661 _2045 Pf6N2E2_2046 - 2-dehydro-3-deoxygluconate kinase (EC 2.7.1.45), at 2,437,860 to 2,438,777 _2046 Pf6N2E2_2047 - D-glucosaminate dehydratase (EC 4.3.1.9) (from data), at 2,438,916 to 2,440,133 _2047 Pf6N2E2_2048 - Transcriptional regulator, IclR family, at 2,440,175 to 2,440,939 _2048 Pf6N2E2_2049 - Bona fide RidA/YjgF/TdcF/RutC subgroup, at 2,441,174 to 2,441,560 _2049 Pf6N2E2_2050 - ABC transporter for D-Glucosamine, putative ATPase component (from data), at 2,441,586 to 2,442,377 _2050 Pf6N2E2_2051 - ABC transporter for D-Glucosamine Hydrochloride, permease component 1 (from data), at 2,442,374 to 2,443,036 _2051 Pf6N2E2_2052 - ABC transporter for D-Glucosamine Hydrochloride, permease component 2 (from data), at 2,443,046 to 2,443,708 _2052
Locus Name Description Strand Phenotype
Pf6N2E2_2042 Acetoacetyl-CoA synthetase (EC 6.2.1.16) + sig.
Pf6N2E2_2043 Twitching motility protein PilT - insig.
Pf6N2E2_2044 Histidine kinase/response regulator hybrid protein + strong
Pf6N2E2_2045 Peptidyl-prolyl cis-trans isomerase PpiC (EC 5.2.1.8) + insig.
Pf6N2E2_2046 2-dehydro-3-deoxygluconate kinase (EC 2.7.1.45) - insig.
Pf6N2E2_2047 D-glucosaminate dehydratase (EC 4.3.1.9) (from data) - strong
Pf6N2E2_2048 Transcriptional regulator, IclR family - sig.
Pf6N2E2_2049 Bona fide RidA/YjgF/TdcF/RutC subgroup - no data
Pf6N2E2_2050 ABC transporter for D-Glucosamine, putative ATPase component (from data) - no data
Pf6N2E2_2051 ABC transporter for D-Glucosamine Hydrochloride, permease component 1 (from data) - strong
Pf6N2E2_2052 ABC transporter for D-Glucosamine Hydrochloride, permease component 2 (from data) - strong

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