Gene info for H281DRAFT_05725 in Paraburkholderia bryophila 376MFSha3.1

Updated annotation (from data): 2,3-dehydroadipyl-CoA hydratase / enoyl-CoA hydratase (EC 4.2.1.17)
Rationale: Specifically important for phenylacetate utilization. 51% identical to the characterized enzyme (paaF = Q845K2) from Pseudomonas sp. Y2, which is part of the aerobic phenylacetyl-CoA pathway. Also, 84% identical to enoyl-CoA hydratase Crt2 = H16_A3307 from Ralstonia eutropha (PMID:30243533).
Original annotation: short chain enoyl-CoA hydratase
Type 1: Protein-coding gene
Located on scaffold H281DRAFT_scaffold00014.14, + strand, nucleotides 145,047 to 145,823
See nucleotide sequence around H281DRAFT_05725 or for all of scaffold H281DRAFT_scaffold00014.14

Other databases: NCBI, uniprot: A0A2Z5MEB0

Nearby Genes

500 ntH281DRAFT_05720 and H281DRAFT_05721 are separated by 201 nucleotidesH281DRAFT_05721 and H281DRAFT_05722 are separated by 1 nucleotidesH281DRAFT_05722 and H281DRAFT_05723 are separated by 53 nucleotidesH281DRAFT_05723 and H281DRAFT_05724 are separated by 121 nucleotidesH281DRAFT_05724 and H281DRAFT_05725 are separated by 131 nucleotides H281DRAFT_05720: H281DRAFT_05720 - phenylacetate-CoA ligase, at 139,050 to 140,354 _05720 H281DRAFT_05721: H281DRAFT_05721 - acyl-CoA thioesterase, at 140,556 to 141,044 _05721 H281DRAFT_05722: H281DRAFT_05722 - 1,2-epoxyphenylacetyl-CoA isomerase (EC 5.3.3.18) (from data), at 141,046 to 141,837 _05722 H281DRAFT_05723: H281DRAFT_05723 - bifunctional thiolase PaaJ: 3-oxo-5,6-didehydrosuberyl-CoA thiolase (EC 2.3.1.223); 3-oxoadipyl-CoA thiolase (EC 2.3.1.174) (from data), at 141,891 to 143,093 _05723 H281DRAFT_05724: H281DRAFT_05724 - 3-oxo-5,6-didehydrosuberyl-CoA semialdehyde dehydrogenase PaaZ (EC:1.2.1.91) (from data), at 143,215 to 144,915 _05724 H281DRAFT_05725: H281DRAFT_05725 - 2,3-dehydroadipyl-CoA hydratase / enoyl-CoA hydratase (EC 4.2.1.17) (from data), at 145,047 to 145,823 _05725
Locus Name Description Strand Phenotype
H281DRAFT_05720 H281DRAFT_05720 phenylacetate-CoA ligase - strong
H281DRAFT_05721 H281DRAFT_05721 acyl-CoA thioesterase - cofit
H281DRAFT_05722 H281DRAFT_05722 1,2-epoxyphenylacetyl-CoA isomerase (EC 5.3.3.18) (from data) - strong
H281DRAFT_05723 H281DRAFT_05723 bifunctional thiolase PaaJ: 3-oxo-5,6-didehydrosuberyl-CoA thiolase (EC 2.3.1.223); 3-oxoadipyl-CoA thiolase (EC 2.3.1.174) (from data) - strong
H281DRAFT_05724 H281DRAFT_05724 3-oxo-5,6-didehydrosuberyl-CoA semialdehyde dehydrogenase PaaZ (EC:1.2.1.91) (from data) - strong
H281DRAFT_05725 H281DRAFT_05725 2,3-dehydroadipyl-CoA hydratase / enoyl-CoA hydratase (EC 4.2.1.17) (from data) + strong

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