Gene info for BWI76_RS03235 in Klebsiella michiganensis M5al

Updated annotation (from data): Neopullulanase (EC 3.2.1.135)
Rationale: Specifically important for utilizing a-Cyclodextrin. Automated validation from mutant phenotype: the predicted function (3.2.1.135) was linked to the condition via a SEED subsystem. This annotation was also checked manually.
Original annotation: alpha-glycosidase
Type 1: Protein-coding gene
Located on scaffold NZ_CP020657, - strand, nucleotides 666,521 to 668,317
See nucleotide sequence around BWI76_RS03235 or for all of scaffold NZ_CP020657

Other databases: NCBI, uniprot: A0A285B8Y2

Nearby Genes

500 ntBWI76_RS03210 and BWI76_RS03215 are separated by 258 nucleotidesBWI76_RS03215 and BWI76_RS03220 are separated by 79 nucleotidesBWI76_RS03220 and BWI76_RS03225 are separated by 429 nucleotidesBWI76_RS03225 and BWI76_RS03230 are separated by 48 nucleotidesBWI76_RS03230 and BWI76_RS03235 overlap by 4 nucleotidesBWI76_RS03235 and BWI76_RS03240 are separated by 14 nucleotidesBWI76_RS03240 and BWI76_RS03245 are separated by 0 nucleotidesBWI76_RS03245 and BWI76_RS03250 are separated by 2 nucleotidesBWI76_RS03250 and BWI76_RS03255 are separated by 419 nucleotidesBWI76_RS03255 and BWI76_RS03260 are separated by 20 nucleotides BWI76_RS03210: BWI76_RS03210 - integrase, at 662,271 to 662,372 _RS03210 BWI76_RS03215: BWI76_RS03215 - hypothetical protein, at 662,631 to 663,731 _RS03215 BWI76_RS03220: BWI76_RS03220 - hypothetical protein, at 663,811 to 664,334 _RS03220 BWI76_RS03225: BWI76_RS03225 - transcriptional regulator, at 664,764 to 665,570 _RS03225 BWI76_RS03230: BWI76_RS03230 - hypothetical protein, at 665,619 to 666,524 _RS03230 BWI76_RS03235: BWI76_RS03235 - Neopullulanase (EC 3.2.1.135) (from data), at 666,521 to 668,317 _RS03235 BWI76_RS03240: BWI76_RS03240 - maltodextrin ABC transporter permease, at 668,332 to 669,165 _RS03240 BWI76_RS03245: BWI76_RS03245 - maltodextrin ABC transporter permease, at 669,166 to 670,449 _RS03245 BWI76_RS03250: BWI76_RS03250 - maltose ABC transporter substrate-binding protein, at 670,452 to 671,681 _RS03250 BWI76_RS03255: BWI76_RS03255 - hypothetical protein, at 672,101 to 673,141 _RS03255 BWI76_RS03260: BWI76_RS03260 - UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase, at 673,162 to 674,169 _RS03260
Locus Name Description Strand Phenotype
BWI76_RS03210 BWI76_RS03210 integrase + no data
BWI76_RS03215 BWI76_RS03215 hypothetical protein + insig.
BWI76_RS03220 BWI76_RS03220 hypothetical protein - insig.
BWI76_RS03225 BWI76_RS03225 transcriptional regulator - strong
BWI76_RS03230 BWI76_RS03230 hypothetical protein - sig.
BWI76_RS03235 BWI76_RS03235 Neopullulanase (EC 3.2.1.135) (from data) - strong
BWI76_RS03240 BWI76_RS03240 maltodextrin ABC transporter permease - strong
BWI76_RS03245 BWI76_RS03245 maltodextrin ABC transporter permease - strong
BWI76_RS03250 BWI76_RS03250 maltose ABC transporter substrate-binding protein - strong
BWI76_RS03255 BWI76_RS03255 hypothetical protein + cofit
BWI76_RS03260 BWI76_RS03260 UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase + strong

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