Gene info for AO356_20240 in Pseudomonas fluorescens FW300-N2C3

Updated annotation (from data): L-arabinose 1-dehydrogenase (EC 1.1.1.46)
Rationale: This is specifically important for L-arabinose utilization. Some closely related proteins in other Pseudomonas are important for D-galactose utilization as well, so this is probably also a D-galactose 1-dehydrogenase. However mutants in this gene had little phenotype in D-galactose (two replicates). No other dehydrogenase was identified as specifically important on D-galactose.
Original annotation: short-chain dehydrogenase
Type 1: Protein-coding gene
Located on scaffold CP012831, + strand, nucleotides 4,524,502 to 4,525,320
See nucleotide sequence around AO356_20240 or for all of scaffold CP012831

Other databases: NCBI, uniprot: A0A0N7H2L2

Nearby Genes

500 ntAO356_20215 and AO356_20220 are separated by 185 nucleotidesAO356_20220 and AO356_20225 are separated by 15 nucleotidesAO356_20225 and AO356_20230 are separated by 292 nucleotidesAO356_20230 and AO356_20235 overlap by 4 nucleotidesAO356_20235 and AO356_20240 are separated by 236 nucleotidesAO356_20240 and AO356_20245 are separated by 174 nucleotidesAO356_20245 and AO356_20250 are separated by 75 nucleotidesAO356_20250 and AO356_20255 are separated by 10 nucleotidesAO356_20255 and AO356_20260 are separated by 148 nucleotidesAO356_20260 and AO356_20265 are separated by 36 nucleotides AO356_20215: AO356_20215 - radical SAM protein, at 4,520,019 to 4,520,987 _20215 AO356_20220: AO356_20220 - hypothetical protein, at 4,521,173 to 4,521,778 _20220 AO356_20225: AO356_20225 - proteophosphoglycan precursor, at 4,521,794 to 4,522,354 _20225 AO356_20230: AO356_20230 - GntR family transcriptional regulator, at 4,522,647 to 4,523,393 _20230 AO356_20235: AO356_20235 - L-arabinolactonase (EC 3.1.1.15) / D-galactonolactonase (EC 3.1.1.25) (from data), at 4,523,390 to 4,524,265 _20235 AO356_20240: AO356_20240 - L-arabinose 1-dehydrogenase (EC 1.1.1.46) (from data), at 4,524,502 to 4,525,320 _20240 AO356_20245: AO356_20245 - sugar ABC transporter substrate-binding protein, at 4,525,495 to 4,526,499 _20245 AO356_20250: AO356_20250 - L-arabinose transporter ATP-binding protein, at 4,526,575 to 4,528,119 _20250 AO356_20255: AO356_20255 - arabinose ABC transporter permease, at 4,528,130 to 4,529,098 _20255 AO356_20260: AO356_20260 - termination factor Rho, at 4,529,247 to 4,529,627 _20260 AO356_20265: AO356_20265 - hypothetical protein, at 4,529,664 to 4,530,008 _20265
Locus Name Description Strand Phenotype
AO356_20215 AO356_20215 radical SAM protein + strong
AO356_20220 AO356_20220 hypothetical protein + strong
AO356_20225 AO356_20225 proteophosphoglycan precursor + insig.
AO356_20230 AO356_20230 GntR family transcriptional regulator + strong
AO356_20235 AO356_20235 L-arabinolactonase (EC 3.1.1.15) / D-galactonolactonase (EC 3.1.1.25) (from data) + strong
AO356_20240 AO356_20240 L-arabinose 1-dehydrogenase (EC 1.1.1.46) (from data) + cofit
AO356_20245 AO356_20245 sugar ABC transporter substrate-binding protein + strong
AO356_20250 AO356_20250 L-arabinose transporter ATP-binding protein + strong
AO356_20255 AO356_20255 arabinose ABC transporter permease + strong
AO356_20260 AO356_20260 termination factor Rho - insig.
AO356_20265 AO356_20265 hypothetical protein - insig.

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