Gene info for AO356_13815 in Pseudomonas fluorescens FW300-N2C3

Updated annotation (from data): ABC transporter for Carnitine, periplasmic substrate-binding component 1
Rationale: Specific phenotype on Carnitine Hydrochloride. This protein is similar to CaiX of P. syringae or P. aeruginosa (Psyr_2916 and PA5388) which has been shown to be a L-carnitine SBP (see PMID:19919675). Another gene in the cluster, AO356_13855, is also a SBP and is specifically important for L-carnitine utilization. AO356_13855 is similar to CbcX of P. aeruginosa or P. syringae (PA5378 or Psyr_4709) which is specific for choline or glycine betaine (ibid). Nevertheless, insertions in _13855 have a specific defect for carnitine utilization. It is possible that these insertions are polar on the beta-cleavage enzyme (_13850) which is required for carnitine catabolism. However insertions in _13855 in either orientation have a strong phenotype.
Original annotation: glycine/betaine ABC transporter substrate-binding protein
Type 1: Protein-coding gene
Located on scaffold CP012831, - strand, nucleotides 3,134,681 to 3,135,628
See nucleotide sequence around AO356_13815 or for all of scaffold CP012831

Other databases: NCBI, uniprot: A0A0N9WVR6

Nearby Genes

500 ntAO356_13790 and AO356_13795 are separated by 147 nucleotidesAO356_13795 and AO356_13800 are separated by 79 nucleotidesAO356_13800 and AO356_13805 are separated by 310 nucleotidesAO356_13805 and AO356_13810 overlap by 4 nucleotidesAO356_13810 and AO356_13815 are separated by 71 nucleotidesAO356_13815 and AO356_13820 are separated by 425 nucleotidesAO356_13820 and AO356_13825 are separated by 617 nucleotidesAO356_13825 and AO356_13830 are separated by 118 nucleotidesAO356_13830 and AO356_13835 are separated by 382 nucleotidesAO356_13835 and AO356_13840 are separated by 19 nucleotides AO356_13790: AO356_13790 - choline dehydrogenase, at 3,128,255 to 3,129,958 _13790 AO356_13795: AO356_13795 - betaine-aldehyde dehydrogenase, at 3,130,106 to 3,131,578 _13795 AO356_13800: AO356_13800 - transcriptional repressor BetI, at 3,131,658 to 3,132,278 _13800 AO356_13805: AO356_13805 - ABC transporter for Carnitine, ATPase component (from data), at 3,132,589 to 3,133,767 _13805 AO356_13810: AO356_13810 - ABC transporter for Carnitine, permease component (from data), at 3,133,764 to 3,134,609 _13810 AO356_13815: AO356_13815 - ABC transporter for Carnitine, periplasmic substrate-binding component 1 (from data), at 3,134,681 to 3,135,628 _13815 AO356_13820: AO356_13820 - serine dehydratase, at 3,136,054 to 3,137,430 _13820 AO356_13825: AO356_13825 - AraC family transcriptional regulator, at 3,138,048 to 3,139,151 _13825 AO356_13830: AO356_13830 - hypothetical protein, at 3,139,270 to 3,139,533 _13830 AO356_13835: AO356_13835 - gamma-butyrobetaine dioxygenase, at 3,139,916 to 3,141,079 _13835 AO356_13840: AO356_13840 - L-carnitine 3-dehydrogenase, thioesterase-like subunit CdhB (EC 1.1.1.108) (from data), at 3,141,099 to 3,141,575 _13840
Locus Name Description Strand Phenotype
AO356_13790 AO356_13790 choline dehydrogenase - strong
AO356_13795 AO356_13795 betaine-aldehyde dehydrogenase - insig.
AO356_13800 AO356_13800 transcriptional repressor BetI - sig.
AO356_13805 AO356_13805 ABC transporter for Carnitine, ATPase component (from data) - strong
AO356_13810 AO356_13810 ABC transporter for Carnitine, permease component (from data) - strong
AO356_13815 AO356_13815 ABC transporter for Carnitine, periplasmic substrate-binding component 1 (from data) - strong
AO356_13820 AO356_13820 serine dehydratase + sig.
AO356_13825 AO356_13825 AraC family transcriptional regulator + strong
AO356_13830 AO356_13830 hypothetical protein - insig.
AO356_13835 AO356_13835 gamma-butyrobetaine dioxygenase - insig.
AO356_13840 AO356_13840 L-carnitine 3-dehydrogenase, thioesterase-like subunit CdhB (EC 1.1.1.108) (from data) - strong

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