Gene info for BT2158 in Bacteroides thetaiotaomicron VPI-5482

Updated annotation (from data): glycoside 3-dehydrogenase (EC 1.1.99.13)
Rationale: This is a glycoside 3-dehydrogenase (PMID:38898276). It was difficult to map insertions in this gene because of a paralog, but reexamination of the data suggests that this gene has a similar fitness pattern as the surrounding genes and is important for growth on trehalose, leucrose, palatinose, and raffinose. And a clean deletion mutant failed to grow on trehalose. It has a TAT (twin arginine transport) signal and is probably exported together with its NAD(P) cofactor, as with the glucose:fructose oxidoreductase Gfo. It's unclear what the electron acceptor could be, given that this enzyme is in the periplasm and B.theta has a very limited electron transport chain. After cleavage by BT2157 (or BT2156, see PMID:38898276) and hydration by BT2157, the resulting 3-ketoglucose is reduced by BT2159, which appears to be cytoplasmic.
Original annotation: putative dehydrogenases and related proteins (NCBI ptt file)
Type 1: Protein-coding gene
Located on scaffold 241, - strand, nucleotides 2,716,447 to 2,717,922
See nucleotide sequence around BT2158 or for all of scaffold 241

Other databases: MicrobesOnline, NCBI, refseq: BT_RS10925, uniprot: Q8A5T0

Nearby Genes

500 ntBT2153 and BT2154 are separated by 109 nucleotidesBT2154 and BT2155 are separated by 159 nucleotidesBT2155 and BT2156 are separated by 68 nucleotidesBT2156 and BT2157 are separated by 139 nucleotidesBT2157 and BT2158 are separated by 52 nucleotidesBT2158 and BT2159 are separated by 7 nucleotidesBT2159 and BT2160 are separated by 252 nucleotidesBT2160 and BT2161 are separated by 41 nucleotidesBT2161 and BT2162 are separated by 14 nucleotidesBT2162 and BT2163 are separated by 2 nucleotides BT2153: BT2153 - putative Fe-S oxidoreductases (NCBI ptt file), at 2,710,904 to 2,712,235 BT2153 BT2154: BT2154 - putative long-chain-fatty-acid--CoA ligase (NCBI ptt file), at 2,712,345 to 2,714,018 BT2154 BT2155: BT2155 - hypothetical protein (NCBI ptt file), at 2,714,178 to 2,714,399 BT2155 BT2156: BT2156 - putative sugar phosphate isomerase/epimerase (NCBI ptt file), at 2,714,468 to 2,715,382 BT2156 BT2157: BT2157 - 3-keto-alpha-glucoside 1,2-lyase; 3-ketoglucose dehydratase (from data), at 2,715,522 to 2,716,394 BT2157 BT2158: BT2158 - glycoside 3-dehydrogenase (EC 1.1.99.13) (from data), at 2,716,447 to 2,717,922 BT2158 BT2159: BT2159 - 3-ketoglucose reductase (NAD(P)H) (from data), at 2,717,930 to 2,719,048 BT2159 BT2160: BT2160 - SusR4: regulator of disaccharide catabolism (SusR4) (from data), at 2,719,301 to 2,720,986 BT2160 BT2161: BT2161 - 50S ribosomal protein L9 (NCBI ptt file), at 2,721,028 to 2,721,471 BT2161 BT2162: BT2162 - 30S ribosomal protein S18 (NCBI ptt file), at 2,721,486 to 2,721,758 BT2162 BT2163: BT2163 - 30S ribosomal protein S6 (NCBI ptt file), at 2,721,761 to 2,722,105 BT2163
Locus Name Description Strand Phenotype
BT2153 BT2153 putative Fe-S oxidoreductases (NCBI ptt file) - strong
BT2154 BT2154 putative long-chain-fatty-acid--CoA ligase (NCBI ptt file) + strong
BT2155 BT2155 hypothetical protein (NCBI ptt file) + strong
BT2156 BT2156 putative sugar phosphate isomerase/epimerase (NCBI ptt file) - insig.
BT2157 BT2157 3-keto-alpha-glucoside 1,2-lyase; 3-ketoglucose dehydratase (from data) - strong
BT2158 BT2158 glycoside 3-dehydrogenase (EC 1.1.99.13) (from data) - no data
BT2159 BT2159 3-ketoglucose reductase (NAD(P)H) (from data) - strong
BT2160 BT2160 SusR4: regulator of disaccharide catabolism (SusR4) (from data) - strong
BT2161 BT2161 50S ribosomal protein L9 (NCBI ptt file) - strong
BT2162 BT2162 30S ribosomal protein S18 (NCBI ptt file) - no data
BT2163 BT2163 30S ribosomal protein S6 (NCBI ptt file) - no data

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