Gene info for BT2157 in Bacteroides thetaiotaomicron VPI-5482

Updated annotation (from data): 3-keto-alpha-glucoside 1,2-lyase; 3-ketoglucose dehydratase
Rationale: Important for the utilization of palatinose, D-trehalose, D-leucrose, and possibly raffinose. This gene is in a conserved cluster for disaccharide catabolism via the 3-ketoglycoside pathway. Originally proposed to be a hydrolase (PMC8121099), it is a lyase, forming 2-hydroxy-3-keto-D-glucal (PMID:38898276, PMID:37932298). It prefers 3-keto-alpha-glycoside substrates over beta substrates (PMID:38898276, PMID:37932298). At a lower rate than the lyase reaction, it hydrates 2-hydroxy-3-keto-D-glucal to 3-ketoglucose (PMID:38898276, PMID:37932298); this activity is also thought to be required for the 3-ketoglycoside pathway, as the 3-ketoglucose is converted to glucose for catabolism (see BT2159). This enzyme is probably in the periplasm.
Original annotation: conserved hypothetical protein (NCBI ptt file)
Type 1: Protein-coding gene
Located on scaffold 241, - strand, nucleotides 2,715,522 to 2,716,394
See nucleotide sequence around BT2157 or for all of scaffold 241

Other databases: MicrobesOnline, NCBI, refseq: BT_RS10920, uniprot: Q8A5T1

Nearby Genes

500 ntBT2152 and BT2153 are separated by 28 nucleotidesBT2153 and BT2154 are separated by 109 nucleotidesBT2154 and BT2155 are separated by 159 nucleotidesBT2155 and BT2156 are separated by 68 nucleotidesBT2156 and BT2157 are separated by 139 nucleotidesBT2157 and BT2158 are separated by 52 nucleotidesBT2158 and BT2159 are separated by 7 nucleotidesBT2159 and BT2160 are separated by 252 nucleotidesBT2160 and BT2161 are separated by 41 nucleotidesBT2161 and BT2162 are separated by 14 nucleotides BT2152: BT2152 - putative acetyltransferase (NCBI ptt file), at 2,710,021 to 2,710,875 BT2152 BT2153: BT2153 - putative Fe-S oxidoreductases (NCBI ptt file), at 2,710,904 to 2,712,235 BT2153 BT2154: BT2154 - putative long-chain-fatty-acid--CoA ligase (NCBI ptt file), at 2,712,345 to 2,714,018 BT2154 BT2155: BT2155 - hypothetical protein (NCBI ptt file), at 2,714,178 to 2,714,399 BT2155 BT2156: BT2156 - putative sugar phosphate isomerase/epimerase (NCBI ptt file), at 2,714,468 to 2,715,382 BT2156 BT2157: BT2157 - 3-keto-alpha-glucoside 1,2-lyase; 3-ketoglucose dehydratase (from data), at 2,715,522 to 2,716,394 BT2157 BT2158: BT2158 - glycoside 3-dehydrogenase (EC 1.1.99.13) (from data), at 2,716,447 to 2,717,922 BT2158 BT2159: BT2159 - 3-ketoglucose reductase (NAD(P)H) (from data), at 2,717,930 to 2,719,048 BT2159 BT2160: BT2160 - SusR4: regulator of disaccharide catabolism (SusR4) (from data), at 2,719,301 to 2,720,986 BT2160 BT2161: BT2161 - 50S ribosomal protein L9 (NCBI ptt file), at 2,721,028 to 2,721,471 BT2161 BT2162: BT2162 - 30S ribosomal protein S18 (NCBI ptt file), at 2,721,486 to 2,721,758 BT2162
Locus Name Description Strand Phenotype
BT2152 BT2152 putative acetyltransferase (NCBI ptt file) - no data
BT2153 BT2153 putative Fe-S oxidoreductases (NCBI ptt file) - strong
BT2154 BT2154 putative long-chain-fatty-acid--CoA ligase (NCBI ptt file) + strong
BT2155 BT2155 hypothetical protein (NCBI ptt file) + strong
BT2156 BT2156 putative sugar phosphate isomerase/epimerase (NCBI ptt file) - insig.
BT2157 BT2157 3-keto-alpha-glucoside 1,2-lyase; 3-ketoglucose dehydratase (from data) - strong
BT2158 BT2158 glycoside 3-dehydrogenase (EC 1.1.99.13) (from data) - no data
BT2159 BT2159 3-ketoglucose reductase (NAD(P)H) (from data) - strong
BT2160 BT2160 SusR4: regulator of disaccharide catabolism (SusR4) (from data) - strong
BT2161 BT2161 50S ribosomal protein L9 (NCBI ptt file) - strong
BT2162 BT2162 30S ribosomal protein S18 (NCBI ptt file) - no data

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