Experiment setAIT092 for Pseudomonas fluorescens FW300-N2E2
Ammonium chloride nitrogen source
Group: nitrogen sourceMedia: RCH2_defined_Glucose_noNitrogen + Ammonium chloride (20 mM)
Culturing: pseudo6_N2E2_ML5, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=500 rpm
Growth: about 4.6 generations
By: Mark on 1/21/2016
Media components: 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 20 mM D-Glucose, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Specific Phenotypes
For 1 genes in this experiment
For nitrogen source Ammonium chloride in Pseudomonas fluorescens FW300-N2E2
For nitrogen source Ammonium chloride across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Chorismate: Intermediate for synthesis of PAPA antibiotics, PABA, anthranilate, 3-hydroxyanthranilate and more. | 1 |
| Tryptophan synthesis | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Phenylalanine, tyrosine and tryptophan biosynthesis
- Biosynthesis of phenylpropanoids
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of plant hormones
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| L-glutamine degradation I | 1 | 1 | 1 |
| L-glutamate biosynthesis I | 2 | 2 | 1 |
| ammonia assimilation cycle III | 3 | 3 | 1 |
| L-asparagine biosynthesis III (tRNA-dependent) | 4 | 4 | 1 |
| glutaminyl-tRNAgln biosynthesis via transamidation | 4 | 4 | 1 |
| acridone alkaloid biosynthesis | 4 | 1 | 1 |
| 4-hydroxy-2(1H)-quinolone biosynthesis | 5 | 2 | 1 |
| L-tryptophan biosynthesis | 6 | 6 | 1 |
| L-glutamate and L-glutamine biosynthesis | 7 | 6 | 1 |
| L-citrulline biosynthesis | 8 | 6 | 1 |
| superpathway of quinolone and alkylquinolone biosynthesis | 10 | 2 | 1 |
| superpathway of L-citrulline metabolism | 12 | 8 | 1 |
| superpathway of L-tryptophan biosynthesis | 13 | 13 | 1 |
| superpathway of aromatic amino acid biosynthesis | 18 | 18 | 1 |
| superpathway of chorismate metabolism | 59 | 43 | 1 |