Experiment setAIT087 for Pseudomonas fluorescens FW300-N2E2

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Parabanic Acid nitrogen source

200 most detrimental genes:

  gene name fitness t score description  
Pf6N2E2_445 +2.3 11.6 UTP--glucose-1-phosphate uridylyltransferase (EC 2.7.7.9) compare
Pf6N2E2_5950 +2.2 8.0 Phosphate:acyl-ACP acyltransferase PlsX compare
Pf6N2E2_3791 +2.2 14.2 Glucans biosynthesis glucosyltransferase H (EC 2.4.1.-) compare
Pf6N2E2_3792 +2.1 10.9 Glucans biosynthesis protein G precursor compare
Pf6N2E2_5705 +2.0 5.6 Sensor protein PhoQ (EC 2.7.13.3) compare
Pf6N2E2_4187 +1.7 6.1 Lipid A biosynthesis lauroyl acyltransferase (EC 2.3.1.-) compare
Pf6N2E2_3271 +1.7 4.2 Phosphocarrier protein, nitrogen regulation associated compare
Pf6N2E2_3667 +1.6 2.2 Heptose kinase WapQ, eukaryotic type compare
Pf6N2E2_3942 +1.5 9.6 FIG001592: Phosphocarrier protein kinase/phosphorylase, nitrogen regulation associated compare
Pf6N2E2_5567 +1.5 2.0 Glutamate Aspartate transport ATP-binding protein GltL (TC 3.A.1.3.4) compare
Pf6N2E2_5623 +1.5 2.7 YciL protein compare
Pf6N2E2_3264 +1.4 2.8 Uncharacterized protein YrbK clustered with lipopolysaccharide transporters compare
Pf6N2E2_3981 +1.4 12.6 T1SS secreted agglutinin RTX compare
Pf6N2E2_3979 +1.4 9.7 Type I secretion system ATPase, LssB family LapB compare
Pf6N2E2_3978 +1.3 10.8 Type I secretion system, membrane fusion protein LapC compare
Pf6N2E2_1774 +1.3 2.4 DNA-binding response regulator, LuxR family, near polyamine transporter compare
Pf6N2E2_4369 +1.3 3.5 Exodeoxyribonuclease III (EC 3.1.11.2) compare
Pf6N2E2_4414 +1.3 2.5 FIG00953808: hypothetical protein compare
Pf6N2E2_3270 +1.2 4.3 Hypothetical ATP-binding protein UPF0042, contains P-loop compare
Pf6N2E2_5499 +1.2 1.6 hypothetical protein compare
Pf6N2E2_5758 +1.2 3.2 FIG00957314: hypothetical protein compare
Pf6N2E2_906 +1.1 3.8 ISPpu14, transposase Orf2 compare
Pf6N2E2_3014 +1.1 1.4 P2-like prophage tail protein X compare
Pf6N2E2_2578 +1.1 3.5 Flagellar biosynthesis protein FliR compare
Pf6N2E2_469 +1.1 1.9 lipoprotein, putative compare
Pf6N2E2_4743 +1.1 1.2 hypothetical protein compare
Pf6N2E2_2201 +1.1 3.9 Transmembrane regulator protein PrtR compare
Pf6N2E2_2912 +1.1 1.9 FIG00954094: hypothetical protein compare
Pf6N2E2_384 +1.1 2.8 Glycine cleavage system transcriptional activator compare
Pf6N2E2_89 +1.1 1.6 hypothetical protein compare
Pf6N2E2_5243 +1.0 2.8 hypothetical protein compare
Pf6N2E2_5573 +1.0 3.8 Glycerol-3-phosphate regulon repressor, DeoR family compare
Pf6N2E2_4057 +1.0 1.3 lipoprotein, putative compare
Pf6N2E2_1248 +1.0 1.7 MutT/nudix family protein compare
Pf6N2E2_5261 +1.0 1.4 FIG00956983: hypothetical protein compare
Pf6N2E2_3300 +1.0 2.2 Predicted D-glucarate or D-galactorate regulator, GntR family compare
Pf6N2E2_146 +1.0 2.5 Transcriptional regulator, MarR family compare
Pf6N2E2_2597 +1.0 6.0 Flagellar regulatory protein FleQ compare
Pf6N2E2_4917 +1.0 5.3 Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1) compare
Pf6N2E2_5993 +1.0 1.8 FIG00957412: hypothetical protein compare
Pf6N2E2_1839 +1.0 2.3 2-hydroxycyclohexanecarboxyl-CoA dehydrogenase (EC 1.1.1.-) compare
Pf6N2E2_3624 +1.0 5.3 Flagellar motor rotation protein MotB compare
Pf6N2E2_6022 +1.0 2.5 StbE replicon stabilization toxin compare
Pf6N2E2_3980 +1.0 6.9 Type I secretion system, outer membrane component LapE compare
Pf6N2E2_5679 +1.0 3.3 hypothetical protein compare
Pf6N2E2_4059 +0.9 1.1 DNA-binding protein HU-alpha compare
Pf6N2E2_924 +0.9 2.7 Transcriptional regulator XdhR, TetR family compare
Pf6N2E2_1027 +0.9 2.4 Cysteine dioxygenase (EC 1.13.11.20) compare
Pf6N2E2_3037 +0.9 6.3 RNA polymerase sigma factor RpoS compare
Pf6N2E2_3325 +0.9 2.1 ABC transporter, ATP-binding protein compare
Pf6N2E2_2278 +0.9 7.3 Transcription-repair coupling factor compare
Pf6N2E2_4365 +0.9 4.4 Ribonuclease PH (EC 2.7.7.56) compare
Pf6N2E2_5134 +0.9 2.8 FIG060329: MOSC domain protein compare
Pf6N2E2_3085 +0.9 1.9 FIG00954614: hypothetical protein compare
Pf6N2E2_3947 +0.8 1.7 Cd(II)/Pb(II)-responsive transcriptional regulator compare
Pf6N2E2_1162 +0.8 1.4 Transmembrane amino acid efflux protein compare
Pf6N2E2_5723 +0.8 5.8 GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase I / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2) compare
Pf6N2E2_2200 +0.8 1.7 RNA polymerase sigma-54 factor RpoN compare
Pf6N2E2_3261 +0.8 2.6 hypothetical protein compare
Pf6N2E2_5172 +0.8 2.5 FOG: TPR repeat compare
Pf6N2E2_2406 +0.8 4.7 Periplasmic binding protein compare
Pf6N2E2_5990 +0.8 1.6 Translation initiation factor 2 (IF-2; GTPase) compare
Pf6N2E2_3727 +0.8 1.8 3-hydroxydecanoyl-[ACP] dehydratase (EC 4.2.1.60) compare
Pf6N2E2_763 +0.8 2.7 Sigma-54 dependent transcriptional regulator compare
Pf6N2E2_3625 +0.8 5.0 Flagellar motor rotation protein MotA compare
Pf6N2E2_2438 +0.8 1.4 hypothetical protein compare
Pf6N2E2_3283 +0.8 3.7 Cytoplasmic axial filament protein CafA and Ribonuclease G (EC 3.1.4.-) compare
Pf6N2E2_3983 +0.8 5.0 Membrane bound c-di-GMP receptor LapD compare
Pf6N2E2_725 +0.8 3.1 Nitrate/nitrite response regulator protein compare
Pf6N2E2_4678 +0.8 1.1 hypothetical protein compare
Pf6N2E2_2041 +0.8 3.5 D-beta-hydroxybutyrate dehydrogenase (EC 1.1.1.30) compare
Pf6N2E2_6047 +0.8 0.9 hypothetical protein compare
Pf6N2E2_5229 +0.8 3.7 Transcriptional regulator, MerR family, associated with photolyase compare
Pf6N2E2_282 +0.7 1.5 GNAT family acetyltransferase YjcF compare
Pf6N2E2_2459 +0.7 1.5 Organic hydroperoxide resistance protein compare
Pf6N2E2_5609 +0.7 3.1 DNA-binding response regulator ColR compare
Pf6N2E2_90 +0.7 3.8 Lipoprotein, putative compare
Pf6N2E2_4733 +0.7 1.3 hypothetical protein compare
Pf6N2E2_5178 +0.7 1.6 CDP-diacylglycerol--serine O-phosphatidyltransferase (EC 2.7.8.8) compare
Pf6N2E2_1506 +0.7 1.8 Putative alkanesulfonate metabolism utilization regulator compare
Pf6N2E2_5771 +0.7 3.4 FKBP-type peptidyl-prolyl cis-trans isomerase FklB (EC 5.2.1.8) compare
Pf6N2E2_1116 +0.7 1.5 Inosine-5'-monophosphate dehydrogenase (EC 1.1.1.205) compare
Pf6N2E2_623 +0.7 1.1 FIG00954674: hypothetical protein compare
Pf6N2E2_546 +0.7 1.2 FIG00966904: hypothetical protein compare
Pf6N2E2_5346 +0.7 2.8 rRNA small subunit methyltransferase I compare
Pf6N2E2_6063 +0.7 3.6 2-methylcitrate dehydratase FeS dependent (EC 4.2.1.79) compare
Pf6N2E2_1492 +0.7 1.6 No significant database matches compare
Pf6N2E2_4537 +0.7 1.6 Type III secretion inner membrane protein (YscS,homologous to flagellar export components) compare
Pf6N2E2_625 +0.7 2.8 MoxR-like ATPase in aerotolerance operon compare
Pf6N2E2_5610 +0.7 3.5 two-component system sensor protein compare
Pf6N2E2_1113 +0.7 1.8 Transcriptional regulator, TetR family compare
Pf6N2E2_4601 +0.7 1.1 Hypothetical protein YggS, proline synthase co-transcribed bacterial homolog PROSC compare
Pf6N2E2_4050 +0.7 1.2 FIG00953718: hypothetical protein compare
Pf6N2E2_2055 +0.7 2.8 StbE replicon stabilization toxin compare
Pf6N2E2_2312 +0.7 1.9 Cytochrome c oxidase subunit CcoQ (EC 1.9.3.1) compare
Pf6N2E2_5086 +0.7 0.9 hypothetical protein compare
Pf6N2E2_1283 +0.7 1.9 Ferric siderophore transport system, periplasmic binding protein TonB compare
Pf6N2E2_5084 +0.6 4.5 Glutathione-regulated potassium-efflux system ATP-binding protein compare
Pf6N2E2_3282 +0.6 1.5 FIG005080: Possible exported protein compare
Pf6N2E2_2313 +0.6 1.6 Cytochrome c oxidase subunit CcoP (EC 1.9.3.1) compare
Pf6N2E2_1714 +0.6 1.9 FIG01218476: hypothetical protein compare
Pf6N2E2_4706 +0.6 1.7 Electron transfer flavoprotein, beta subunit compare
Pf6N2E2_1617 +0.6 0.8 COG2199: FOG: GGDEF domain compare
Pf6N2E2_2589 +0.6 2.1 Flagellar protein FliJ compare
Pf6N2E2_5695 +0.6 3.4 Flagellar basal-body rod protein FlgC compare
Pf6N2E2_2806 +0.6 1.5 Methyltransferase (EC 2.1.1.-) compare
Pf6N2E2_1106 +0.6 2.3 Chromosome initiation inhibitor compare
Pf6N2E2_1442 +0.6 3.3 Transcriptional regulators of sugar metabolism compare
Pf6N2E2_74 +0.6 1.9 DedD protein compare
Pf6N2E2_1153 +0.6 2.2 small molecule metabolism; degradation; fatty acid compare
Pf6N2E2_3870 +0.6 1.8 hypothetical protein compare
Pf6N2E2_3472 +0.6 1.6 Probable short-chain dehydrogenase compare
Pf6N2E2_4362 +0.6 1.6 DNA-directed RNA polymerase omega subunit (EC 2.7.7.6) compare
Pf6N2E2_3362 +0.6 2.0 hypothetical protein compare
Pf6N2E2_5697 +0.6 4.6 Flagellar hook protein FlgE compare
Pf6N2E2_5230 +0.6 4.2 COG1683: Uncharacterized conserved protein / FIG143828: Hypothetical protein YbgA compare
Pf6N2E2_58 +0.6 1.7 tRNA-dihydrouridine synthase C (EC 1.-.-.-) compare
Pf6N2E2_1099 +0.6 1.2 Hypothetical protein GlcG in glycolate utilization operon compare
Pf6N2E2_2582 +0.6 1.1 Flagellar motor switch protein FliN compare
Pf6N2E2_4545 +0.6 1.2 hypothetical protein compare
Pf6N2E2_2590 +0.6 3.8 Flagellum-specific ATP synthase FliI compare
Pf6N2E2_4599 +0.6 1.4 Integral membrane protein YggT, involved in response to extracytoplasmic stress (osmotic shock) compare
Pf6N2E2_1526 +0.6 1.9 MgtC family compare
Pf6N2E2_1110 +0.6 2.0 FIG00964414: hypothetical protein compare
Pf6N2E2_3763 +0.6 1.0 hypothetical protein compare
Pf6N2E2_3021 +0.6 0.9 hypothetical protein compare
Pf6N2E2_3560 +0.6 2.8 membrane protein, putative compare
Pf6N2E2_328 +0.6 2.7 hypothetical protein compare
Pf6N2E2_4991 +0.6 2.9 DedA protein compare
Pf6N2E2_3547 +0.6 3.7 Urea ABC transporter, substrate binding protein UrtA compare
Pf6N2E2_118 +0.6 3.5 FIG00961753: hypothetical protein compare
Pf6N2E2_689 +0.6 1.2 FIG00956189: hypothetical protein compare
Pf6N2E2_1141 +0.6 1.6 Lipid-A-disaccharide synthase (EC 2.4.1.182) compare
Pf6N2E2_5696 +0.6 2.4 Flagellar basal-body rod modification protein FlgD compare
Pf6N2E2_4139 +0.6 4.2 Integral membrane protein TerC compare
Pf6N2E2_4766 +0.6 4.2 Acyl-CoA dehydrogenase (EC 1.3.8.7) compare
Pf6N2E2_2934 +0.6 2.3 lipoprotein, putative compare
Pf6N2E2_5722 +0.6 1.6 23S rRNA (Uracil-5-) -methyltransferase RumA (EC 2.1.1.-) ## LSU rRNA m(5)U1939 compare
Pf6N2E2_5961 +0.6 3.4 Transcriptional regulator, TetR family compare
Pf6N2E2_1470 +0.6 2.2 Transcriptional regulator, LysR family compare
Pf6N2E2_5250 +0.6 1.6 Restriction endonuclease compare
Pf6N2E2_756 +0.6 2.2 hypothetical protein compare
Pf6N2E2_39 +0.6 1.9 FIG00857858: hypothetical protein compare
Pf6N2E2_1860 +0.5 1.1 Ferredoxin, 2Fe-2S compare
Pf6N2E2_4926 +0.5 3.9 hypothetical protein compare
Pf6N2E2_2152 +0.5 0.8 hypothetical protein compare
Pf6N2E2_1215 +0.5 1.5 probable membrane protein YPO2362 compare
Pf6N2E2_1104 +0.5 2.9 Gluconate dehydratase (EC 4.2.1.39) compare
Pf6N2E2_5625 +0.5 3.0 COG0613, Predicted metal-dependent phosphoesterases (PHP family) compare
Pf6N2E2_2587 +0.5 4.1 Serine phosphatase RsbU, regulator of sigma subunit compare
Pf6N2E2_182 +0.5 4.4 Two-component hybrid sensor and regulator compare
Pf6N2E2_2999 +0.5 1.0 probable tail fiber assembly protein compare
Pf6N2E2_3321 +0.5 1.9 Ornithine cyclodeaminase (EC 4.3.1.12) compare
Pf6N2E2_5236 +0.5 1.4 hypothetical protein compare
Pf6N2E2_2362 +0.5 2.5 Type III secretion inner membrane protein (YscU,SpaS,EscU,HrcU,SsaU, homologous to flagellar export components) compare
Pf6N2E2_5569 +0.5 3.5 Glutamate Aspartate transport system permease protein GltJ (TC 3.A.1.3.4) compare
Pf6N2E2_1978 +0.5 1.6 DNA topoisomerase III (EC 5.99.1.2) in PFGI-1-like cluster compare
Pf6N2E2_4715 +0.5 1.4 Low-specificity L-threonine aldolase (EC 4.1.2.48) compare
Pf6N2E2_5640 +0.5 1.2 FIG00955597: hypothetical protein compare
Pf6N2E2_1206 +0.5 2.0 protein of unknown function UPF0153 compare
Pf6N2E2_3179 +0.5 1.6 FIG021952: putative membrane protein compare
Pf6N2E2_1091 +0.5 1.6 Flavodoxin reductases (ferredoxin-NADPH reductases) family 1; Vanillate O-demethylase oxidoreductase (EC 1.14.13.-) compare
Pf6N2E2_2034 +0.5 1.0 Methyltransferase compare
Pf6N2E2_1032 +0.5 0.7 Alkanesulfonates ABC transporter ATP-binding protein / Sulfonate ABC transporter, ATP-binding subunit SsuB compare
Pf6N2E2_5491 +0.5 3.6 FOG: GGDEF domain compare
Pf6N2E2_1448 +0.5 1.4 hypothetical protein compare
Pf6N2E2_6064 +0.5 2.4 2-methylaconitate isomerase compare
Pf6N2E2_5568 +0.5 2.8 Glutamate Aspartate transport system permease protein GltK (TC 3.A.1.3.4) compare
Pf6N2E2_2579 +0.5 2.1 Flagellar biosynthesis protein FliQ compare
Pf6N2E2_243 +0.5 2.3 L-proline glycine betaine binding ABC transporter protein ProX (TC 3.A.1.12.1) compare
Pf6N2E2_1181 +0.5 2.3 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100) compare
Pf6N2E2_4028 +0.5 3.2 Biofilm PGA synthesis auxiliary protein PgaD compare
Pf6N2E2_5023 +0.5 1.9 Thiamin-phosphate pyrophosphorylase (EC 2.5.1.3) compare
Pf6N2E2_3982 +0.5 1.1 Tryptophan synthase beta chain like (EC 4.2.1.20) compare
Pf6N2E2_2825 +0.5 2.2 RND efflux system, outer membrane lipoprotein CmeC compare
Pf6N2E2_2331 +0.5 2.8 FIG00954563: hypothetical protein compare
Pf6N2E2_1493 +0.5 1.9 ABC-type polar amino acid transport system, ATPase component compare
Pf6N2E2_5116 +0.5 2.7 Type IV fimbrial biogenesis protein PilW compare
Pf6N2E2_33 +0.5 1.2 Diaminopimelate epimerase (EC 5.1.1.7) compare
Pf6N2E2_1983 +0.5 1.1 hypothetical protein compare
Pf6N2E2_1395 +0.5 2.1 Uncharacterized conserved protein compare
Pf6N2E2_4478 +0.5 2.9 FIG019278: hypothetical protein compare
Pf6N2E2_4030 +0.5 3.6 Biofilm PGA synthesis deacetylase PgaB (EC 3.-) compare
Pf6N2E2_40 +0.5 1.9 Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1) compare
Pf6N2E2_510 +0.5 3.2 Predicted transcriptional regulator of the myo-inositol catabolic operon compare
Pf6N2E2_1534 +0.5 0.8 Probable transmembrane protein compare
Pf6N2E2_3344 +0.5 1.5 N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28) AmpD compare
Pf6N2E2_1761 +0.5 1.5 Transcriptional regulator, LysR family compare
Pf6N2E2_5589 +0.5 2.7 Sodium-type flagellar protein motY precursor compare
Pf6N2E2_309 +0.5 0.8 FIG00957702: hypothetical protein compare
Pf6N2E2_5168 +0.5 1.6 Regulator of competence-specific genes compare
Pf6N2E2_5057 +0.5 1.5 hypothetical protein compare
Pf6N2E2_586 +0.5 2.8 Membrane-bound lytic murein transglycosylase D precursor (EC 3.2.1.-) compare
Pf6N2E2_4906 +0.5 2.4 hypothetical protein compare
Pf6N2E2_460 +0.5 1.2 Glycerate kinase (EC 2.7.1.31) compare
Pf6N2E2_2350 +0.5 1.7 Oxygen-regulated invasion protein OrgB compare
Pf6N2E2_5671 +0.5 1.3 Succinylglutamate desuccinylase (EC 3.5.1.96) compare
Pf6N2E2_2487 +0.5 0.6 FIG00956090: hypothetical protein compare
Pf6N2E2_4615 +0.5 0.7 type IV pili signal transduction protein PilI compare
Pf6N2E2_4570 +0.5 1.7 Cation/multidrug efflux pump compare


Specific Phenotypes

For 6 genes in this experiment

For nitrogen source Parabanic Acid in Pseudomonas fluorescens FW300-N2E2

For nitrogen source Parabanic Acid across organisms