Experiment setAIT077 for Pseudomonas fluorescens FW300-N2E2

Compare to:

L-Alanine nitrogen source

200 most important genes:

  gene name fitness t score description  
Pf6N2E2_4597 -6.3 -6.2 Homoserine O-acetyltransferase (EC 2.3.1.31) compare
Pf6N2E2_3248 -6.1 -5.9 Probable transmembrane protein compare
Pf6N2E2_3253 -5.8 -5.7 ATP phosphoribosyltransferase (EC 2.4.2.17) (from data) compare
Pf6N2E2_3783 -5.8 -5.7 phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31) (from data) compare
Pf6N2E2_77 -5.8 -4.0 O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) / O-succinylhomoserine sulfhydrylase (EC 2.5.1.48) compare
Pf6N2E2_71 -5.7 -5.6 Phosphoribosylanthranilate isomerase (EC 5.3.1.24) compare
Pf6N2E2_4825 -5.6 -8.6 Anthranilate phosphoribosyltransferase (EC 2.4.2.18) compare
Pf6N2E2_4826 -5.4 -7.4 Indole-3-glycerol phosphate synthase (EC 4.1.1.48) compare
Pf6N2E2_4279 -5.4 -11.6 Pyruvate carboxyl transferase subunit B (EC 6.4.1.1) compare
Pf6N2E2_3842 -5.4 -5.3 Imidazoleglycerol-phosphate dehydratase (EC 4.2.1.19) compare
Pf6N2E2_4596 -5.4 -9.7 Homoserine O-acetyltransferase (EC 2.3.1.31) compare
Pf6N2E2_3751 -5.4 -12.5 Glutamate synthase [NADPH] small chain (EC 1.4.1.13) compare
Pf6N2E2_3630 -5.3 -7.2 Phosphoserine phosphatase (EC 3.1.3.3) compare
Pf6N2E2_4762 -5.3 -5.1 Biotin synthesis protein BioH compare
Pf6N2E2_3839 -5.1 -3.5 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) (from data) compare
Pf6N2E2_50 -5.1 -9.1 Biosynthetic Aromatic amino acid aminotransferase alpha (EC 2.6.1.57) @ Aromatic-amino-acid aminotransferase (EC 2.6.1.57) compare
Pf6N2E2_4047 -5.1 -4.9 Tryptophan synthase alpha chain (EC 4.2.1.20) compare
Pf6N2E2_4278 -5.0 -13.0 Pyruvate carboxyl transferase subunit A (EC 6.4.1.1) compare
Pf6N2E2_3932 -5.0 -7.6 D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95) compare
Pf6N2E2_3752 -4.9 -21.2 Glutamate synthase [NADPH] large chain (EC 1.4.1.13) compare
Pf6N2E2_3782 -4.9 -3.3 Phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19) compare
Pf6N2E2_2073 -4.8 -4.7 Sulfite reductase [NADPH] hemoprotein beta-component (EC 1.8.1.2) compare
Pf6N2E2_5177 -4.8 -12.7 Ketol-acid reductoisomerase (EC 1.1.1.86) compare
Pf6N2E2_5176 -4.8 -9.1 Acetolactate synthase small subunit (EC 2.2.1.6) compare
Pf6N2E2_5175 -4.7 -13.6 Acetolactate synthase large subunit (EC 2.2.1.6) compare
Pf6N2E2_64 -4.6 -4.7 3-isopropylmalate dehydratase small subunit (EC 4.2.1.33) compare
Pf6N2E2_3251 -4.6 -11.8 histidinol-phosphate aminotransferase (EC 2.6.1.9) (from data) compare
Pf6N2E2_4759 -4.6 -16.5 Biotin synthase (EC 2.8.1.6) compare
Pf6N2E2_4048 -4.5 -8.8 Tryptophan synthase beta chain (EC 4.2.1.20) compare
Pf6N2E2_4390 -4.5 -11.1 Leucine-responsive regulatory protein, regulator for leucine (or lrp) regulon and high-affinity branched-chain amino acid transport system conserved
Pf6N2E2_3252 -4.3 -9.9 Histidinol dehydrogenase (EC 1.1.1.23) compare
Pf6N2E2_3940 -4.3 -13.0 Phosphoserine phosphatase (EC 3.1.3.3) compare
Pf6N2E2_5527 -4.2 -2.9 Cold shock protein CspC compare
Pf6N2E2_4564 -4.2 -9.4 Dihydroxy-acid dehydratase (EC 4.2.1.9) compare
Pf6N2E2_5006 -4.1 -10.6 Muramoyltetrapeptide carboxypeptidase (EC 3.4.17.13) compare
Pf6N2E2_4391 -4.1 -7.3 D-amino acid dehydrogenase small subunit (EC 1.4.99.1) compare
Pf6N2E2_3841 -4.1 -6.2 Imidazole glycerol phosphate synthase amidotransferase subunit (EC 2.4.2.-) compare
Pf6N2E2_2074 -4.0 -2.4 required for sulfate utilization, putative electron transport protein for sulfite reductase (from data) compare
Pf6N2E2_4418 -4.0 -1.7 Diaminopimelate epimerase (EC 5.1.1.7) compare
Pf6N2E2_66 -3.9 -10.3 3-isopropylmalate dehydrogenase (EC 1.1.1.85) compare
Pf6N2E2_3610 -3.7 -4.4 ATP phosphoribosyltransferase regulatory subunit (EC 2.4.2.17) compare
Pf6N2E2_4277 -3.7 -10.6 LysR family transcriptional regulator PA5437 compare
Pf6N2E2_2258 -3.7 -13.9 Transaldolase (EC 2.2.1.2) compare
Pf6N2E2_2465 -3.5 -6.8 Cys regulon transcriptional activator CysB compare
Pf6N2E2_4761 -3.5 -13.8 8-amino-7-oxononanoate synthase (EC 2.3.1.47) compare
Pf6N2E2_2885 -3.4 -8.7 Glucose-6-phosphate 1-dehydrogenase (EC 1.1.1.49) compare
Pf6N2E2_4763 -3.3 -11.1 Biotin synthesis protein BioC compare
Pf6N2E2_3826 -3.3 -7.3 Nitrogen regulation protein NR(I) compare
Pf6N2E2_4764 -3.3 -7.9 Dethiobiotin synthetase (EC 6.3.3.3) compare
Pf6N2E2_4071 -3.2 -6.0 Phosphate transport system regulatory protein PhoU compare
Pf6N2E2_5950 -3.2 -5.1 Phosphate:acyl-ACP acyltransferase PlsX compare
Pf6N2E2_2513 -3.0 -5.9 UDP-glucose dehydrogenase (EC 1.1.1.22) compare
Pf6N2E2_5723 -3.0 -14.0 GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase I / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2) compare
Pf6N2E2_4631 -3.0 -9.9 Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (EC 2.6.1.62) compare
Pf6N2E2_3941 -2.9 -4.7 Adenosine (5')-pentaphospho-(5'')-adenosine pyrophosphohydrolase (EC 3.6.1.-) compare
Pf6N2E2_5156 -2.8 -2.7 Two-component sensor CbrB: intrcellular carbon:nitrogen balance compare
Pf6N2E2_5579 -2.8 -7.3 Ornithine carbamoyltransferase (EC 2.1.3.3) compare
Pf6N2E2_2753 -2.7 -3.6 HtrA protease/chaperone protein compare
Pf6N2E2_3170 -2.7 -8.1 2-isopropylmalate synthase (EC 2.3.3.13) compare
Pf6N2E2_5676 -2.7 -3.4 Aspartokinase (EC 2.7.2.4) compare
Pf6N2E2_5667 -2.6 -9.7 Arginine N-succinyltransferase (EC 2.3.1.109) compare
Pf6N2E2_5666 -2.6 -11.3 Arginine N-succinyltransferase, alpha subunit (EC 2.3.1.109) compare
Pf6N2E2_4392 -2.6 -3.3 Translation initiation inhibitor compare
Pf6N2E2_51 -2.6 -6.5 hypothetical protein compare
Pf6N2E2_3264 -2.5 -2.8 Uncharacterized protein YrbK clustered with lipopolysaccharide transporters compare
Pf6N2E2_3825 -2.5 -5.1 Nitrogen regulation protein NtrB (EC 2.7.13.3) compare
Pf6N2E2_4187 -2.5 -4.9 Lipid A biosynthesis lauroyl acyltransferase (EC 2.3.1.-) compare
Pf6N2E2_1717 -2.5 -9.3 Anthranilate synthase, aminase component (EC 4.1.3.27) compare
Pf6N2E2_3460 -2.4 -2.2 Exodeoxyribonuclease V beta chain (EC 3.1.11.5) compare
Pf6N2E2_302 -2.3 -5.8 Siroheme synthase / Precorrin-2 oxidase (EC 1.3.1.76) / Sirohydrochlorin ferrochelatase (EC 4.99.1.4) / Uroporphyrinogen-III methyltransferase (EC 2.1.1.107) compare
Pf6N2E2_4987 -2.3 -1.9 MaoC-like domain protein compare
Pf6N2E2_63 -2.2 -2.9 3-isopropylmalate dehydratase large subunit (EC 4.2.1.33) compare
Pf6N2E2_3257 -2.2 -4.6 Uncharacterized ABC transporter, auxiliary component YrbC compare
Pf6N2E2_4803 -2.1 -2.3 Bis(5'-nucleosyl)-tetraphosphatase, symmetrical (EC 3.6.1.41) compare
Pf6N2E2_4206 -2.1 -2.5 Chromosome (plasmid) partitioning protein ParB compare
Pf6N2E2_4797 -2.1 -2.3 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase (EC 2.7.6.3) compare
Pf6N2E2_1718 -2.1 -1.8 Anthranilate synthase, amidotransferase component (EC 4.1.3.27) compare
Pf6N2E2_4638 -2.1 -3.4 5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20) compare
Pf6N2E2_3984 -2.1 -10.0 T1SS associated transglutaminase-like cysteine proteinase LapP compare
Pf6N2E2_2425 -2.1 -3.9 Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1) compare
Pf6N2E2_4352 -2.0 -3.0 ATP-dependent DNA helicase RecG (EC 3.6.1.-) compare
Pf6N2E2_5103 -2.0 -1.5 Glutamate 5-kinase (EC 2.7.2.11) / RNA-binding C-terminal domain PUA compare
Pf6N2E2_5001 -2.0 -2.0 D-alanyl-D-alanine carboxypeptidase (EC 3.4.16.4) compare
Pf6N2E2_5014 -1.9 -1.5 Apolipoprotein N-acyltransferase (EC 2.3.1.-) / Copper homeostasis protein CutE compare
Pf6N2E2_4417 -1.9 -5.0 Protein of unknown function DUF484 compare
Pf6N2E2_5252 -1.8 -1.6 FIG00956396: hypothetical protein compare
Pf6N2E2_2218 -1.8 -4.4 ATP-dependent Clp protease proteolytic subunit (EC 3.4.21.92) compare
Pf6N2E2_3938 -1.8 -11.0 Threonine dehydratase biosynthetic (EC 4.3.1.19) compare
Pf6N2E2_3661 -1.8 -5.2 hypothetical protein compare
Pf6N2E2_5746 -1.7 -2.6 Cobalamin synthase compare
Pf6N2E2_1056 -1.7 -1.6 Quinone oxidoreductase (EC 1.6.5.5) compare
Pf6N2E2_5316 -1.7 -2.7 Periplasmic thiol:disulfide oxidoreductase DsbB, required for DsbA reoxidation compare
Pf6N2E2_3786 -1.7 -4.5 Twin-arginine translocation protein TatC compare
Pf6N2E2_1089 -1.7 -3.5 Delta 1-piperideine-2-carboxylate reductase (EC 1.5.1.21) / Delta 1-pyrroline-2-carboxylate reductase (EC 1.5.1.1) compare
Pf6N2E2_5190 -1.6 -9.2 Carbonic anhydrase (EC 4.2.1.1) compare
Pf6N2E2_3875 -1.6 -1.8 FIG00953324: hypothetical protein compare
Pf6N2E2_4009 -1.6 -6.9 GGDEF domain protein compare
Pf6N2E2_5160 -1.6 -1.3 C4-type zinc finger protein, DksA/TraR family compare
Pf6N2E2_3607 -1.6 -7.6 3'-to-5' exoribonuclease RNase R compare
Pf6N2E2_4453 -1.6 -10.8 Polyphosphate kinase (EC 2.7.4.1) compare
Pf6N2E2_6090 -1.6 -2.7 Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain compare
Pf6N2E2_2510 -1.5 -3.0 Lipid carrier : UDP-N-acetylgalactosaminyltransferase (EC 2.4.1.-) / Alpha-1,3-N-acetylgalactosamine transferase PglA (EC 2.4.1.-); Putative glycosyltransferase compare
Pf6N2E2_5669 -1.5 -6.7 Succinylarginine dihydrolase (EC 3.5.3.23) compare
Pf6N2E2_2897 -1.5 -8.0 Glucokinase (EC 2.7.1.2) compare
Pf6N2E2_3027 -1.5 -1.8 Baseplate assembly protein V compare
Pf6N2E2_3537 -1.5 -4.3 Cobalt-precorrin-6x reductase (EC 1.3.1.54) compare
Pf6N2E2_613 -1.5 -1.8 lipoprotein, putative compare
Pf6N2E2_3206 -1.5 -1.2 hypothetical protein compare
Pf6N2E2_5331 -1.4 -6.3 ATPase, AFG1 family compare
Pf6N2E2_5679 -1.4 -3.0 hypothetical protein compare
Pf6N2E2_3198 -1.4 -1.9 tRNA-guanine transglycosylase (EC 2.4.2.29) compare
Pf6N2E2_3305 -1.4 -3.5 hypothetical protein compare
Pf6N2E2_4372 -1.4 -3.9 FIG00460773: hypothetical protein compare
Pf6N2E2_3039 -1.4 -5.1 Protein-L-isoaspartate O-methyltransferase (EC 2.1.1.77) compare
Pf6N2E2_2852 -1.4 -7.0 GGDEF domain/EAL domain protein compare
Pf6N2E2_288 -1.4 -2.4 Isocitrate dehydrogenase [NADP] (EC 1.1.1.42); Monomeric isocitrate dehydrogenase [NADP] (EC 1.1.1.42) compare
Pf6N2E2_3942 -1.4 -8.3 FIG001592: Phosphocarrier protein kinase/phosphorylase, nitrogen regulation associated compare
Pf6N2E2_5219 -1.3 -6.6 FIG140336: TPR domain protein compare
Pf6N2E2_4399 -1.3 -4.1 GGDEF domain/EAL domain protein compare
Pf6N2E2_4416 -1.3 -2.4 Tyrosine recombinase XerC compare
Pf6N2E2_6140 -1.3 -1.0 Large Subunit Ribosomal RNA; lsuRNA; LSU rRNA compare
Pf6N2E2_3072 -1.3 -6.7 [Protein-PII] uridylyltransferase (EC 2.7.7.59) compare
Pf6N2E2_2248 -1.3 -8.7 Aspartate racemase compare
Pf6N2E2_3134 -1.3 -1.2 hypothetical protein compare
Pf6N2E2_4160 -1.2 -1.2 Cytochrome c5 compare
Pf6N2E2_2124 -1.2 -1.7 Mg(2+) transport ATPase protein C compare
Pf6N2E2_902 -1.2 -2.5 hypothetical protein compare
Pf6N2E2_3261 -1.2 -3.6 hypothetical protein compare
Pf6N2E2_4636 -1.2 -5.2 periplasmic binding protein, putative compare
Pf6N2E2_3328 -1.2 -2.5 transcriptional regulator compare
Pf6N2E2_2318 -1.2 -5.4 Type cbb3 cytochrome oxidase biogenesis protein CcoI; Copper-translocating P-type ATPase (EC 3.6.3.4) compare
Pf6N2E2_2312 -1.2 -1.7 Cytochrome c oxidase subunit CcoQ (EC 1.9.3.1) compare
Pf6N2E2_4824 -1.2 -3.0 Anthranilate synthase, amidotransferase component (EC 4.1.3.27) @ Para-aminobenzoate synthase, amidotransferase component (EC 2.6.1.85) compare
Pf6N2E2_3534 -1.2 -2.0 Cobalamin biosynthesis protein CobG compare
Pf6N2E2_2536 -1.1 -1.2 Fumarate hydratase class II (EC 4.2.1.2) compare
Pf6N2E2_4276 -1.1 -4.2 Phosphogluconate repressor HexR, RpiR family compare
Pf6N2E2_3349 -1.1 -1.4 Polyribonucleotide nucleotidyltransferase (EC 2.7.7.8) compare
Pf6N2E2_3535 -1.1 -3.9 Cobalt-precorrin-6y C5-methyltransferase (EC 2.1.1.-) / Cobalt-precorrin-6y C15-methyltransferase [decarboxylating] (EC 2.1.1.-) compare
Pf6N2E2_3461 -1.1 -1.5 Exodeoxyribonuclease V gamma chain (EC 3.1.11.5) compare
Pf6N2E2_3040 -1.1 -4.1 5-nucleotidase SurE (EC 3.1.3.5) compare
Pf6N2E2_5155 -1.1 -2.5 Poly(A) polymerase (EC 2.7.7.19) compare
Pf6N2E2_1431 -1.1 -2.5 Branched-chain amino acid transport system permease protein LivM (TC 3.A.1.4.1) compare
Pf6N2E2_2261 -1.1 -4.9 VacJ-like lipoprotein precursor compare
Pf6N2E2_2527 -1.1 -3.3 Oxidoreductase, short chain dehydrogenase/reductase family compare
Pf6N2E2_5338 -1.1 -5.6 Ubiquinol--cytochrome c reductase, cytochrome B subunit (EC 1.10.2.2) compare
Pf6N2E2_1128 -1.1 -2.0 Two-component response regulator compare
Pf6N2E2_3820 -1.1 -1.2 hypothetical protein compare
Pf6N2E2_5337 -1.1 -3.0 Ubiquinol-cytochrome C reductase iron-sulfur subunit (EC 1.10.2.2) compare
Pf6N2E2_828 -1.0 -2.7 Transcriptional regulator, TetR family compare
Pf6N2E2_865 -1.0 -1.6 Fumarylacetoacetate hydrolase family protein compare
Pf6N2E2_2072 -1.0 -2.2 hypothetical protein compare
Pf6N2E2_2675 -1.0 -1.0 ATP-dependent RNA helicase SrmB compare
Pf6N2E2_2413 -1.0 -1.9 FIG074102: hypothetical protein compare
Pf6N2E2_3901 -1.0 -2.3 Ribosome-associated heat shock protein implicated in the recycling of the 50S subunit (S4 paralog) compare
Pf6N2E2_658 -1.0 -5.6 sensor histidine kinase compare
Pf6N2E2_3908 -1.0 -4.3 Glutamate--cysteine ligase (EC 6.3.2.2) compare
Pf6N2E2_2588 -1.0 -3.0 Anti-sigma F factor antagonist (spoIIAA-2); Anti-sigma B factor antagonist RsbV compare
Pf6N2E2_1605 -1.0 -5.1 Carboxynorspermidine dehydrogenase, putative (EC 1.1.1.-) compare
Pf6N2E2_441 -1.0 -2.1 FIG00958773: hypothetical protein compare
Pf6N2E2_37 -1.0 -2.5 6-carboxytetrahydropterin synthase (EC 4.1.2.50) @ Queuosine biosynthesis QueD, PTPS-I compare
Pf6N2E2_5258 -1.0 -4.8 Sulfate adenylyltransferase subunit 1 (EC 2.7.7.4) / Adenylylsulfate kinase (EC 2.7.1.25) compare
Pf6N2E2_70 -1.0 -2.8 tRNA pseudouridine synthase A (EC 4.2.1.70) compare
Pf6N2E2_4360 -1.0 -5.9 Bona fide RidA/YjgF/TdcF/RutC subgroup compare
Pf6N2E2_2402 -1.0 -1.6 hypothetical protein compare
Pf6N2E2_2217 -1.0 -2.6 ATP-dependent Clp protease ATP-binding subunit ClpX compare
Pf6N2E2_525 -1.0 -3.9 C4-type zinc finger protein, DksA/TraR family compare
Pf6N2E2_4928 -1.0 -1.4 Permeases of the major facilitator superfamily compare
Pf6N2E2_2309 -1.0 -1.7 hypothetical protein compare
Pf6N2E2_4007 -1.0 -2.5 FIG00956018: hypothetical protein compare
Pf6N2E2_5508 -0.9 -1.8 Formyltetrahydrofolate deformylase (EC 3.5.1.10) compare
Pf6N2E2_4273 -0.9 -4.8 ATP-dependent DNA helicase UvrD/PcrA compare
Pf6N2E2_3611 -0.9 -1.2 HflC protein compare
Pf6N2E2_3634 -0.9 -1.7 Transporter compare
Pf6N2E2_4205 -0.9 -3.2 Chromosome (plasmid) partitioning protein ParA compare
Pf6N2E2_4159 -0.9 -2.7 Cytochrome c4 compare
Pf6N2E2_2065 -0.9 -2.6 Cobalt-precorrin-4 C11-methyltransferase (EC 2.1.1.133) compare
Pf6N2E2_2518 -0.9 -1.3 Cyclohexadienyl dehydrogenase (EC 1.3.1.12)(EC 1.3.1.43) / 5-Enolpyruvylshikimate-3-phosphate synthase (EC 2.5.1.19) compare
Pf6N2E2_2674 -0.9 -1.2 FIG00953856: hypothetical protein compare
Pf6N2E2_2010 -0.9 -1.5 hypothetical protein compare
Pf6N2E2_4063 -0.9 -2.0 Chorismate--pyruvate lyase (EC 4.1.3.40) compare
Pf6N2E2_2080 -0.9 -0.9 hypothetical protein compare
Pf6N2E2_2125 -0.9 -3.3 hypothetical protein compare
Pf6N2E2_2017 -0.9 -1.9 DNA-binding protein compare
Pf6N2E2_4710 -0.9 -2.0 hypothetical protein compare
Pf6N2E2_5738 -0.9 -4.4 Cobyrinic acid A,C-diamide synthase compare
Pf6N2E2_2514 -0.9 -2.6 regulator of length of O-antigen component of lipopolysaccharide chains compare
Pf6N2E2_3477 -0.9 -1.6 2-Oxobutyrate oxidase, putative compare
Pf6N2E2_3720 -0.9 -2.0 FIG027190: Putative transmembrane protein compare
Pf6N2E2_703 -0.9 -2.9 Cytochrome c55X precursor NirC compare
Pf6N2E2_5743 -0.9 -3.2 Adenosylcobinamide-phosphate guanylyltransferase (EC 2.7.7.62) compare
Pf6N2E2_2899 -0.9 -2.8 NAD-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12) compare
Pf6N2E2_1691 -0.9 -1.9 hypothetical protein compare
Pf6N2E2_3260 -0.9 -2.6 Uncharacterized ABC transporter, ATP-binding protein YrbF compare
Pf6N2E2_2889 -0.9 -4.8 Glucose ABC transporter, ATP-binding subunit (EC 3.6.3.-) compare
Pf6N2E2_4601 -0.9 -1.5 Hypothetical protein YggS, proline synthase co-transcribed bacterial homolog PROSC compare
Pf6N2E2_4394 -0.9 -3.9 Putrescine utilization regulator compare
Pf6N2E2_3677 -0.9 -6.4 Sensory box/GGDEF family protein compare
Pf6N2E2_1083 -0.9 -1.0 hypothetical protein compare
Pf6N2E2_2064 -0.9 -2.1 Cobalt-precorrin-3b C17-methyltransferase compare
Pf6N2E2_5841 -0.9 -0.9 GlcG protein compare


Specific Phenotypes

For 3 genes in this experiment

For nitrogen source L-Alanine in Pseudomonas fluorescens FW300-N2E2

For nitrogen source L-Alanine across organisms