Experiment setAIT072 for Pseudomonas fluorescens FW300-N2E2

Compare to:

L-Serine nitrogen source

200 most detrimental genes:

  gene name fitness t score description  
Pf6N2E2_4684 +6.6 4.1 hypothetical protein compare
Pf6N2E2_4683 +5.8 8.7 L-proline glycine betaine binding ABC transporter protein ProX (TC 3.A.1.12.1) compare
Pf6N2E2_3978 +5.8 40.9 Type I secretion system, membrane fusion protein LapC compare
Pf6N2E2_3979 +5.7 36.1 Type I secretion system ATPase, LssB family LapB compare
Pf6N2E2_2022 +5.3 13.5 BarA-associated response regulator UvrY (= GacA = SirA) compare
Pf6N2E2_3981 +5.1 40.1 T1SS secreted agglutinin RTX compare
Pf6N2E2_5720 +4.8 21.8 BarA sensory histidine kinase (= VarS = GacS) compare
Pf6N2E2_3980 +4.7 28.4 Type I secretion system, outer membrane component LapE compare
Pf6N2E2_1981 +4.2 7.1 FIG051360: Periplasmic protein TonB, links inner and outer membranes compare
Pf6N2E2_4086 +4.0 32.0 sodium/alanine transporter compare
Pf6N2E2_122 +3.8 5.4 Tyrosine-protein kinase Wzc (EC 2.7.10.2) compare
Pf6N2E2_2436 +3.5 4.3 DNA-binding response regulator, LuxR family compare
Pf6N2E2_2755 +3.2 7.6 Sigma factor RpoE negative regulatory protein RseA compare
Pf6N2E2_5559 +3.2 6.3 Glycine cleavage system H protein compare
Pf6N2E2_3983 +3.0 11.9 Membrane bound c-di-GMP receptor LapD compare
Pf6N2E2_4075 +2.8 9.8 Phosphate ABC transporter, periplasmic phosphate-binding protein PstS (TC 3.A.1.7.1) compare
Pf6N2E2_4391 +2.6 13.7 D-amino acid dehydrogenase small subunit (EC 1.4.99.1) compare
Pf6N2E2_4084 +2.6 12.7 Transcriptional regulator compare
Pf6N2E2_3908 +2.6 12.5 Glutamate--cysteine ligase (EC 6.3.2.2) compare
Pf6N2E2_3611 +2.5 4.3 HflC protein compare
Pf6N2E2_5510 +2.5 1.7 FIG00955324: hypothetical protein compare
Pf6N2E2_4351 +2.4 10.9 Predicted signal transduction protein compare
Pf6N2E2_5406 +2.3 6.1 FIG00958224: hypothetical protein compare
Pf6N2E2_4204 +2.3 2.9 rRNA small subunit 7-methylguanosine (m7G) methyltransferase GidB compare
Pf6N2E2_3624 +2.2 10.2 Flagellar motor rotation protein MotB compare
Pf6N2E2_3612 +2.2 4.5 HflK protein compare
Pf6N2E2_4087 +2.1 8.7 L-asparaginase (EC 3.5.1.1) (from data) conserved
Pf6N2E2_3625 +2.1 12.7 Flagellar motor rotation protein MotA compare
Pf6N2E2_2438 +2.0 4.1 hypothetical protein compare
Pf6N2E2_5705 +2.0 2.5 Sensor protein PhoQ (EC 2.7.13.3) compare
Pf6N2E2_70 +2.0 5.2 tRNA pseudouridine synthase A (EC 4.2.1.70) compare
Pf6N2E2_2597 +2.0 12.8 Flagellar regulatory protein FleQ compare
Pf6N2E2_906 +2.0 3.6 ISPpu14, transposase Orf2 compare
Pf6N2E2_2217 +1.9 5.8 ATP-dependent Clp protease ATP-binding subunit ClpX compare
Pf6N2E2_4148 +1.9 10.6 Glutamate-aspartate carrier protein compare
Pf6N2E2_2406 +1.9 10.3 Periplasmic binding protein compare
Pf6N2E2_43 +1.9 6.5 Histidine permease YuiF compare
Pf6N2E2_5664 +1.8 7.4 Arginine pathway regulatory protein ArgR, repressor of arg regulon compare
Pf6N2E2_2175 +1.8 7.8 Transcriptional regulator, RpiR family compare
Pf6N2E2_4599 +1.8 3.0 Integral membrane protein YggT, involved in response to extracytoplasmic stress (osmotic shock) compare
Pf6N2E2_3192 +1.8 4.3 tRNA:Cm32/Um32 methyltransferase compare
Pf6N2E2_2587 +1.8 12.2 Serine phosphatase RsbU, regulator of sigma subunit compare
Pf6N2E2_3621 +1.8 10.1 putative transporter, required for glycine utilization (from data) compare
Pf6N2E2_2178 +1.8 5.9 FIG00960144: hypothetical protein compare
Pf6N2E2_5237 +1.7 2.8 Hypoxanthine-guanine phosphoribosyltransferase (EC 2.4.2.8) compare
Pf6N2E2_2756 +1.7 6.7 RNA polymerase sigma-H factor AlgT compare
Pf6N2E2_3179 +1.7 2.8 FIG021952: putative membrane protein compare
Pf6N2E2_3303 +1.7 4.4 Sensor histidine kinase PrrB (RegB) (EC 2.7.3.-) compare
Pf6N2E2_5025 +1.7 14.2 Sensor histidine kinase compare
Pf6N2E2_2277 +1.7 3.1 NADPH-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.13) compare
Pf6N2E2_6058 +1.6 6.7 FIG139976: hypothetical protein compare
Pf6N2E2_5610 +1.6 7.3 two-component system sensor protein compare
Pf6N2E2_2106 +1.6 1.4 hypothetical protein compare
Pf6N2E2_5860 +1.6 9.0 Branched-chain amino acid transport system carrier protein compare
Pf6N2E2_5556 +1.6 6.4 Aminomethyltransferase (EC 2.1.2.10) (from data) compare
Pf6N2E2_4451 +1.6 8.0 FIG139438: lipoprotein B compare
Pf6N2E2_728 +1.5 1.7 Nitrate/nitrite transporter compare
Pf6N2E2_445 +1.5 6.1 UTP--glucose-1-phosphate uridylyltransferase (EC 2.7.7.9) compare
Pf6N2E2_2057 +1.5 7.6 Phosphatidylserine/phosphatidylglycerophosphate/ cardiolipi n synthases and related enzymes compare
Pf6N2E2_4702 +1.5 9.2 DgcA Dimethylglycine demethylase subunit A conserved
Pf6N2E2_4612 +1.5 5.8 Glutathione synthetase (EC 6.3.2.3) compare
Pf6N2E2_5491 +1.4 8.3 FOG: GGDEF domain compare
Pf6N2E2_3792 +1.4 6.9 Glucans biosynthesis protein G precursor compare
Pf6N2E2_2441 +1.4 6.1 putative ATP/GTP-binding protein compare
Pf6N2E2_4464 +1.4 1.8 2-polyprenylphenol hydroxylase and related flavodoxin oxidoreductases / CDP-6-deoxy-delta-3,4-glucoseen reductase-like compare
Pf6N2E2_5172 +1.4 2.4 FOG: TPR repeat compare
Pf6N2E2_4486 +1.4 6.0 Glycine cleavage system H protein compare
Pf6N2E2_1759 +1.4 1.6 uncharacterized domain 1 compare
Pf6N2E2_4193 +1.4 9.1 Signal transduction histidine kinase compare
Pf6N2E2_3982 +1.4 3.6 Tryptophan synthase beta chain like (EC 4.2.1.20) compare
Pf6N2E2_4706 +1.3 2.1 Electron transfer flavoprotein, beta subunit compare
Pf6N2E2_4153 +1.3 1.5 Sensory box histidine kinase compare
Pf6N2E2_4926 +1.3 8.3 hypothetical protein compare
Pf6N2E2_89 +1.3 1.5 hypothetical protein compare
Pf6N2E2_3791 +1.3 7.0 Glucans biosynthesis glucosyltransferase H (EC 2.4.1.-) compare
Pf6N2E2_4030 +1.3 8.6 Biofilm PGA synthesis deacetylase PgaB (EC 3.-) compare
Pf6N2E2_170 +1.3 2.6 lipoprotein, putative compare
Pf6N2E2_4928 +1.3 2.3 Permeases of the major facilitator superfamily compare
Pf6N2E2_377 +1.3 4.2 hypothetical protein compare
Pf6N2E2_4527 +1.3 0.9 RNA polymerase sigma-70 factor, ECF subfamily compare
Pf6N2E2_5661 +1.3 3.4 L-Arginine ABC transporter, permease protein AotQ (from data) compare
Pf6N2E2_5609 +1.3 4.6 DNA-binding response regulator ColR compare
Pf6N2E2_5743 +1.3 6.2 Adenosylcobinamide-phosphate guanylyltransferase (EC 2.7.7.62) compare
Pf6N2E2_4335 +1.3 3.8 Uncharacterized protein ImpB compare
Pf6N2E2_1889 +1.3 2.6 Permease of the drug/metabolite transporter (DMT) superfamily compare
Pf6N2E2_4682 +1.3 3.8 ABC transporter for Carnitine, permease component (from data) compare
Pf6N2E2_5874 +1.3 9.7 TonB-dependent receptor; Outer membrane receptor for ferrienterochelin and colicins compare
Pf6N2E2_3613 +1.3 7.0 GTP-binding protein HflX compare
Pf6N2E2_2320 +1.3 3.5 Heavy-metal-associated domain (N-terminus) and membrane-bounded cytochrome biogenesis cycZ-like domain, possible membrane copper tolerance protein compare
Pf6N2E2_4640 +1.3 1.7 hypothetical protein compare
Pf6N2E2_3667 +1.3 1.4 Heptose kinase WapQ, eukaryotic type compare
Pf6N2E2_5257 +1.2 7.1 Sulfate adenylyltransferase subunit 2 (EC 2.7.7.4) compare
Pf6N2E2_4333 +1.2 4.1 Uncharacterized protein ImpD compare
Pf6N2E2_2605 +1.2 4.2 3-oxoacyl-[acyl-carrier-protein] synthase, KASIII (EC 2.3.1.180) compare
Pf6N2E2_4181 +1.2 1.6 Organic hydroperoxide resistance protein compare
Pf6N2E2_2061 +1.2 3.9 CobW GTPase involved in cobalt insertion for B12 biosynthesis compare
Pf6N2E2_5662 +1.2 4.8 L-Arginine ABC transporter, permease protein AotM (from data) compare
Pf6N2E2_2646 +1.2 2.3 FIG00953416: hypothetical protein compare
Pf6N2E2_4494 +1.2 2.4 Adenylate cyclase (EC 4.6.1.1) compare
Pf6N2E2_339 +1.2 2.0 Glutathione-regulated potassium-efflux system protein KefC compare
Pf6N2E2_2292 +1.2 3.7 Universal stress protein family COG0589 compare
Pf6N2E2_4031 +1.2 8.9 Biofilm PGA outer membrane secretin PgaA compare
Pf6N2E2_5300 +1.2 7.7 Transcriptional regulator, GntR family domain / Aspartate aminotransferase (EC 2.6.1.1) compare
Pf6N2E2_942 +1.2 1.7 Mll6465 protein compare
Pf6N2E2_4340 +1.1 3.7 Outer membrane protein ImpK/VasF, OmpA/MotB domain compare
Pf6N2E2_2919 +1.1 1.7 FIG00955830: hypothetical protein compare
Pf6N2E2_4028 +1.1 6.0 Biofilm PGA synthesis auxiliary protein PgaD compare
Pf6N2E2_4390 +1.1 5.9 Leucine-responsive regulatory protein, regulator for leucine (or lrp) regulon and high-affinity branched-chain amino acid transport system compare
Pf6N2E2_4593 +1.1 3.7 Radical SAM family enzyme, similar to coproporphyrinogen III oxidase, oxygen-independent, clustered with nucleoside-triphosphatase RdgB compare
Pf6N2E2_4337 +1.1 7.7 Uncharacterized protein ImpI/VasC compare
Pf6N2E2_5402 +1.1 5.7 ABC transporter for D-Alanine, periplasmic substrate-binding component (from data) conserved
Pf6N2E2_1723 +1.1 1.5 Transcriptional regulator, TetR family compare
Pf6N2E2_5663 +1.1 3.4 L-Arginine ABC transporter, ATPase component (from data) compare
Pf6N2E2_3658 +1.1 3.2 Lipid A core - O-antigen ligase and related enzymes compare
Pf6N2E2_1173 +1.1 2.4 hypothetical protein compare
Pf6N2E2_1248 +1.1 1.7 MutT/nudix family protein compare
Pf6N2E2_1435 +1.1 2.8 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases; sulfonate monooxygenase compare
Pf6N2E2_1966 +1.1 2.0 SAM-dependent methyltransferases compare
Pf6N2E2_4194 +1.1 6.3 Two-component response regulator compare
Pf6N2E2_4339 +1.1 6.0 Uncharacterized protein ImpJ/VasE compare
Pf6N2E2_1821 +1.1 1.6 Leucine-responsive regulatory protein, regulator for leucine (or lrp) regulon and high-affinity branched-chain amino acid transport system compare
Pf6N2E2_3198 +1.1 1.3 tRNA-guanine transglycosylase (EC 2.4.2.29) compare
Pf6N2E2_3761 +1.1 8.7 Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-) compare
Pf6N2E2_2252 +1.1 2.4 NreA-like protein compare
Pf6N2E2_3062 +1.0 4.2 Membrane-associated zinc metalloprotease compare
Pf6N2E2_4662 +1.0 2.7 hypothetical protein compare
Pf6N2E2_4367 +1.0 1.9 hypothetical protein compare
Pf6N2E2_2561 +1.0 1.7 FIG00955434: hypothetical protein compare
Pf6N2E2_1464 +1.0 2.3 hypothetical protein compare
Pf6N2E2_61 +1.0 5.9 Cys regulon transcriptional activator CysB compare
Pf6N2E2_2447 +1.0 5.0 Probable protease htpX homolog (EC 3.4.24.-) compare
Pf6N2E2_924 +1.0 2.2 Transcriptional regulator XdhR, TetR family compare
Pf6N2E2_3475 +1.0 1.3 hypothetical protein compare
Pf6N2E2_5064 +1.0 3.7 Xanthine/uracil/thiamine/ascorbate permease family protein compare
Pf6N2E2_5258 +1.0 4.3 Sulfate adenylyltransferase subunit 1 (EC 2.7.7.4) / Adenylylsulfate kinase (EC 2.7.1.25) compare
Pf6N2E2_595 +1.0 3.1 Arsenical resistance operon repressor compare
Pf6N2E2_4074 +1.0 6.2 Phosphate transport system permease protein PstC (TC 3.A.1.7.1) compare
Pf6N2E2_3255 +1.0 2.0 YrbA protein compare
Pf6N2E2_3029 +1.0 1.7 Holin compare
Pf6N2E2_5459 +1.0 5.4 D-serine/D-alanine/glycine transporter compare
Pf6N2E2_5873 +1.0 4.3 FIG00953467: hypothetical protein compare
Pf6N2E2_2503 +1.0 1.2 Glycosyl transferase, group 1 family protein compare
Pf6N2E2_2288 +1.0 2.8 FIG00953423: hypothetical protein compare
Pf6N2E2_2717 +1.0 2.4 putative cytoplasmic protein compare
Pf6N2E2_4029 +1.0 3.9 Biofilm PGA synthesis N-glycosyltransferase PgaC (EC 2.4.-.-) compare
Pf6N2E2_4821 +1.0 5.0 Phosphoglycolate phosphatase (EC 3.1.3.18) compare
Pf6N2E2_2329 +0.9 4.4 diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s) compare
Pf6N2E2_1181 +0.9 3.2 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100) compare
Pf6N2E2_314 +0.9 2.0 Isochorismatase (EC 3.3.2.1) compare
Pf6N2E2_4334 +0.9 5.0 Uncharacterized protein ImpC compare
Pf6N2E2_3532 +0.9 4.4 Cobalt-precorrin-2 C20-methyltransferase (EC 2.1.1.130) compare
Pf6N2E2_1729 +0.9 3.1 FIG00464907: hypothetical protein compare
Pf6N2E2_1596 +0.9 1.5 Periplasmic nitrate reductase component NapD compare
Pf6N2E2_1446 +0.9 2.6 Enoyl-[acyl-carrier-protein] reductase [NADPH] (EC 1.3.1.10) compare
Pf6N2E2_5299 +0.9 3.3 Benzoate transport protein compare
Pf6N2E2_2507 +0.9 4.1 Undecaprenyl-phosphate N-acetylglucosaminyl 1-phosphate transferase (EC 2.7.8.-) compare
Pf6N2E2_4329 +0.9 3.7 Protein ImpG/VasA compare
Pf6N2E2_5181 +0.9 6.2 Paraquat-inducible protein A compare
Pf6N2E2_249 +0.9 2.9 Pirin compare
Pf6N2E2_546 +0.9 1.4 FIG00966904: hypothetical protein compare
Pf6N2E2_4341 +0.9 7.0 IcmF-related protein compare
Pf6N2E2_3003 +0.9 1.8 Bacteriophage protein GP46 compare
Pf6N2E2_2317 +0.9 2.6 Putative analog of CcoH, COG3198 compare
Pf6N2E2_5945 +0.9 2.9 Similar to phosphoglycolate phosphatase, clustered with ribosomal large subunit pseudouridine synthase C compare
Pf6N2E2_4679 +0.9 4.1 Betaine aldehyde dehydrogenase (EC 1.2.1.8) compare
Pf6N2E2_156 +0.9 1.3 Carbon storage regulator compare
Pf6N2E2_5403 +0.9 4.6 ABC transporter for D-Alanine, permease component 2 (from data) compare
Pf6N2E2_2894 +0.9 2.3 N-acylglucosamine 2-epimerase (EC 5.1.3.8) compare
Pf6N2E2_3969 +0.9 1.1 Membrane-fusion protein compare
Pf6N2E2_4996 +0.9 2.0 LSU m3Psi1915 methyltransferase RlmH compare
Pf6N2E2_5298 +0.9 2.6 Sarcosine oxidase beta subunit (EC 1.5.3.1) compare
Pf6N2E2_5182 +0.9 3.5 Paraquat-inducible protein A compare
Pf6N2E2_2572 +0.9 1.7 Chemotaxis regulator - transmits chemoreceptor signals to flagelllar motor components CheY compare
Pf6N2E2_3397 +0.9 2.4 Phage protein compare
Pf6N2E2_4710 +0.9 0.8 hypothetical protein compare
Pf6N2E2_3666 +0.9 1.6 InaA protein compare
Pf6N2E2_3456 +0.9 3.9 Inner membrane protein YfiN compare
Pf6N2E2_1815 +0.9 2.1 Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases compare
Pf6N2E2_2177 +0.9 2.7 Glutamine synthetase (EC 6.3.1.2), putative compare
Pf6N2E2_5078 +0.9 1.2 Carbon starvation protein A compare
Pf6N2E2_4704 +0.9 5.1 Dimethylglycine dehydrogenase (ferredoxin), FeS subunit DgcB (EC 1.5.7.-) (from data) compare
Pf6N2E2_2714 +0.8 1.6 transcriptional regulator, Crp/Fnr family compare
Pf6N2E2_5153 +0.8 3.3 3-methyl-2-oxobutanoate hydroxymethyltransferase (EC 2.1.2.11) compare
Pf6N2E2_2633 +0.8 1.0 Nitroreductase family protein compare
Pf6N2E2_413 +0.8 1.9 Chemotaxis response regulator protein-glutamate methylesterase CheB (EC 3.1.1.61) compare
Pf6N2E2_5536 +0.8 1.2 Holliday junction DNA helicase RuvA compare
Pf6N2E2_6052 +0.8 1.9 Hydantoin racemase (EC 5.1.99.-) compare
Pf6N2E2_5296 +0.8 4.6 Threonine synthase (EC 4.2.3.1) compare
Pf6N2E2_2235 +0.8 2.2 Xanthine permease compare
Pf6N2E2_5660 +0.8 3.0 L-Arginine ABC transporter, periplasmic substrate-binding component (from data) compare
Pf6N2E2_3055 +0.8 1.2 Ribonuclease HII (EC 3.1.26.4) compare
Pf6N2E2_1223 +0.8 1.3 Amino acid transporters compare
Pf6N2E2_3537 +0.8 2.9 Cobalt-precorrin-6x reductase (EC 1.3.1.54) compare
Pf6N2E2_180 +0.8 1.2 Transcriptional regulator, MerR family compare
Pf6N2E2_3455 +0.8 1.2 Integral membrane protein YfiB compare
Pf6N2E2_1691 +0.8 1.5 hypothetical protein compare
Pf6N2E2_3546 +0.8 2.8 ABC transporter (iron.B12.siderophore.hemin) , permease component compare
Pf6N2E2_1493 +0.8 2.2 ABC-type polar amino acid transport system, ATPase component compare
Pf6N2E2_5297 +0.8 3.9 RidA/YER057c/UK114 superfamily, group 2, YoaB-like protein compare
Pf6N2E2_4332 +0.8 3.0 hypothetical protein compare


Specific Phenotypes

For 33 genes in this experiment

For nitrogen source L-Serine in Pseudomonas fluorescens FW300-N2E2

For nitrogen source L-Serine across organisms